Skip to main content

Advertisement

SpringerLink
Log in

Benign odontogenic ghost cell lesions revisited and new considerations on dysplastic dentin

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

This study aimed to revisit benign odontogenic ghost cell lesions (BOGCL) by hematoxylin and eosin staining and immunohistochemistry.

Materials and methods

Thirty cases of calcifying odontogenic cyst (COC) and 6 cases of dentinogenic ghost cell tumor (DGCT) were selected for histopathological and immunohistochemical analysis. Sections stained for cytokeratin (K) 14, K-19, amelogenin, collagen type 1 (COL-1), and dentin matrix acidic phosphoprotein 1 (DMP-1) were evaluated using qualitative analysis. Sections stained for Ki-67 and minichromosome maintenance protein-2 (MCM-2) were evaluated using semi-quantitative analysis.

Results

A morphologic overlap was noticed in all BOGCL. Moreover, no differences were detected in the expression of K-14 and K-19. The expression of proliferative markers Ki-67 and MCM-2 was similar between cystic and tumor lesions (p > .05). The presence of COL-1 and absence of amelogenin in the so-called dysplastic dentin, associated with its histologic pattern, suggest that this is in fact an enameloid-like tissue.

Conclusions

The dysplastic dentin should be considered an enameloid-like tissue in these lesions.

Clinical relevance

The similarity in histology, protein expression, and proliferative marker indices between COC and DGCT suggest that they are a sole entity and likely represent types of the same neoplasia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. El-Naggar AK, Chan JKC, Grandis JR, Takata T, Slootweg PJ (eds) (2017) World Health Organization classification of tumours. WHO classification of head and neck tumours. IARC Press, Lyon

    Google Scholar 

  2. Gorlin RJ, Pindborg JJ, Clausen FP, Vickers RA (1962) The calcifying odontogenic cyst—a possible analogue of the cutaneous calcifying epithelioma of Malherbe. An analysis of fifteen cases. Oral Surg Oral Med Oral Pathol 15:1235–1243

    Article  Google Scholar 

  3. Praetorius F, Hjørting-Hansen E, Gorlin RJ, Vickers RA (1981) Calcifying odontogenic cyst. Range, variations and neoplastic potential. Acta Odontol Scand 39(4):227–240

    Article  Google Scholar 

  4. Barnes L, Eveson JW, Reichart P, Sidransky D (eds) (2005) World Health Organization classification of tumours: pathology and genetics, head and neck tumours. IARC Press, Lyon

    Google Scholar 

  5. Ledesma-Montes C, Gorlin RJ, Shear M, Praétorius F, Mosqueda-Taylor A, Altini M, Unni K, Paes de Almeida O, Carlos-Bregni R, Romero de León E, Phillips V, Delgado-Azañero W, Meneses-García A (2008) International collaborative study on ghost cell odontogenic tumours: calcifying cystic odontogenic tumour, dentinogenic ghost cell tumour and ghost cell odontogenic carcinoma. J Oral Pathol Med 37(5):302–308

    Article  Google Scholar 

  6. Jones AV, Craig GT, Franklin CD (2006) Range and demographics of odontogenic cysts diagnosed in a UK population over a 30-year period. J Oral Pathol Med 35(8):500–507

    Article  Google Scholar 

  7. Hong SP, Ellis GL, Hartman KS (1991) Calcifying odontogenic cyst. A review of ninety-two cases with reevaluation of their nature as cysts or neoplasms, the nature of ghost cells, and subclassification. Oral Surg Oral Med Oral Pathol 72(1):56–64

    Article  Google Scholar 

  8. Toida M (1998) So-called calcifying odontogenic cyst: review and discussion on the terminology and classification. J Oral Pathol Med. 27(2):49–52

    Article  Google Scholar 

  9. Domingues MG, Jaeger MM, Araújo VC, Araújo NS (2000) Expression of cytokeratins in human enamel organ. Eur J Oral Sci 108(1):43–47

    Article  Google Scholar 

  10. Yukimori A, Oikawa Y, Morita KI, Nguyen CTK, Harada H, Yamaguchi S, Kayamori K, Yamaguchi A, Ikeda T, Sakamoto K (2017) Genetic basis of calcifying cystic odontogenic tumors. PLoS One 12(6):e0180224

    Article  Google Scholar 

  11. Chrcanovic BR, Gomez RS (2016) Peripheral calcifying cystic odontogenic tumour and peripheral dentinogenic ghost cell tumour: an updated systematic review of 117 cases reported in the literature. Acta Odontol Scand 74(8):591–597

    Article  Google Scholar 

  12. ivan V, Altini M, Meer S (2008) Secretory cells in adenomatoid odontogenic tumour: tissue induction or metaplastic mineralisation? Oral Dis 14(5):445–449

    Article  Google Scholar 

  13. Assaraf-Weill N, Gasse B, Silvent J, Bardet C, Sire JY, Davit-Béal T (2014) Ameloblasts express type I collagen during amelogenesis. J Dent Res 93(5):502–507

    Article  Google Scholar 

  14. Sire JY, Kawasaki K (2012) Origin and evolution of bone and dentin and of acid secretory calcium binding phosphoproteins. In: Goldberg M (ed) Frontiers between science and clinic in odontology, volume 2. INSERM Université Paris Descartes, France

    Google Scholar 

  15. Kogaya Y (1999) Immunohistochemical localisation of amelogenin-like proteins and type I collagen and histochemical demonstration of sulphated glycoconjugates in developing enameloid and enamel matrices of the larval urodele (Triturus pyrrhogaster) teeth. J Anat 195:455–464

    Article  Google Scholar 

  16. Kawasaki K, Suzuki T, Weiss KM (2005) Phenogenetic drift in evolution: the changing genetic basis of vertebrate teeth. Proc Natl Acad Sci U S A 102(50):18063–18068

    Article  Google Scholar 

  17. Sasagawa I, Ishiyama M, Yokosuka H, Mikami M, Oka S, Shimokawa H, Uchida T (2018) Immunolocalization of enamel matrix protein-like proteins in the tooth enameloid of spotted gar, Lepisosteus oculatus, an actinopterygian bony fish. Connect Tissue Res:1–13. https://doi.org/10.1080/03008207.2018.1506446

  18. Crivelini MM, de Araújo VC, de Sousa SO, de Araújo NS (2003) Cytokeratins in epithelia of odontogenic neoplasms. Oral Dis 9(1):1–6

    Article  Google Scholar 

  19. Crivelini MM, Felipini RC, Coclete GA, Soubhia AM (2009) Immunoexpression of keratins in the calcifying cystic odontogenic tumor epithelium. J Oral Pathol Med 38(4):393–396

    Article  Google Scholar 

  20. Kaminagakura E, Domingos PL, da Rosa MR et al (2013) Detection of cytokeratins in ghost cells of calcifying cystic odontogenic tumor indicates an altered keratinization and hair follicle differentiation for their development. Ann Diagn Pathol 17(6):514–517

    Article  Google Scholar 

  21. Wang Y, Azaïs T, Robin M, Vallée A, Catania C, Legriel P, Pehau-Arnaudet G, Babonneau F, Giraud-Guille MM, Nassif N (2012) The predominant role of collagen in the nucleation, growth, structure and orientation of bone apatite. Nat Mater 11(8):724–733

    Article  Google Scholar 

  22. Landis WJ, Jacquet R (2013) Association of calcium and phosphate ions with collagen in the mineralization of vertebrate tissues. Calcif Tissue Int 93(4):329–337

    Article  Google Scholar 

  23. Martinez EF, da Silva LA, Furuse C, de Araújo NS, de Araújo VC (2009) Dentin matrix protein 1 (DMP1) expression in developing human teeth. Braz Dent J 20(5):365–369

    Article  Google Scholar 

  24. Inagaki Y, Kashima TG, Hookway ES, Tanaka Y, Hassan AB, Oppermann U, Athanasou NA (2015) Dentine matrix protein 1 (DMP-1) is a marker of bone formation and mineralisation in soft tissue tumours. Virchows Arch 466(4):445–452

    Article  Google Scholar 

  25. Gajjeraman S, Narayanan K, Hao J, Qin C, George A (2007) Matrix macromolecules in hard tissues control the nucleation and hierarchical assembly of hydroxyapatite. J Biol Chem 282(2):1193–1204

    Article  Google Scholar 

  26. Tanaka A, Okamoto M, Yoshizawa D, Ito S, Alva PG, Ide F, Kusama K (2007) Presence of ghost cells and the Wnt signaling pathway in odontomas. J Oral Pathol Med 36(7):400–404

    Article  Google Scholar 

  27. Slootweg PJ (1995) p53 protein and Ki-67 reactivity in epithelial odontogenic lesions. An immunohistochemical study. J Oral Pathol Med 24(9):393–397

    Article  Google Scholar 

  28. Gong Y, Wang L, Wang H, Li T, Chen X (2009) The expression of NF-kappaB, Ki-67 and MMP-9 in CCOT, DGCT and GCOC. Oral Oncol 45(6):515–520

    Article  Google Scholar 

  29. Carreón-Burciaga RG, González-González R, Molina-Frechero N, Bologna-Molina R (2015) Immunoexpression of Ki-67, MCM2, and MCM3 in ameloblastoma and ameloblastic carcinoma and their correlations with clinical and histopathological patterns. Dis Markers:683087. https://doi.org/10.1155/2015/683087

  30. Güler N, Comunoğlu N, Cabbar F (2012) Ki-67 and MCM-2 in dental follicle and odontogenic cysts: the effects of inflammation on proliferative markers. Sci World J:946060. https://doi.org/10.1100/2012/946060

Download references

Acknowledgments

The authors wish to thank Nadir Severina de Freitas and Ronyere Olegário de Araújo for their excellent technical expertise and assistance. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. School of Medicine, Federal University of Tocantins, Quadra 109 Norte, Avenida NS-15, ALCNO-14, Plano Diretor Norte, Palmas, Tocantins, 77001-090, Brazil

    Ana Cláudia Garcia Rosa

  2. Faculty of Human and Economics and Health Sciences ITPAC, School of Medicine and Dentistry, Palmas, TO, Brazil

    Ana Cláudia Garcia Rosa

  3. São Leopoldo Mandic Institute and Research Center, Oral Pathology, Campinas, SP, Brazil

    Lucas Novaes Teixeira, Fabricio Passador-Santos, Victor Ângelo Martins Montalli, Ney Soares de Araújo & Vera Cavalcanti de Araújo

  4. Pathology and Clinical Propaedeutics, São Paulo State University, Araçatuba, SP, Brazil

    Cristiane Furuse

Authors
  1. Ana Cláudia Garcia Rosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lucas Novaes Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fabricio Passador-Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cristiane Furuse
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Victor Ângelo Martins Montalli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ney Soares de Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Vera Cavalcanti de Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ana Cláudia Garcia Rosa.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study has been approved by the human research ethics committee of São Leopoldo Mandic Dental Institute and Research Center (#89616218.7.0000.5374) and has been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments. This article does not contain any studies with animals performed by any of the authors.

Informed consent

For this type of study, formal consent is not required.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rosa, A.C.G., Teixeira, L.N., Passador-Santos, F. et al. Benign odontogenic ghost cell lesions revisited and new considerations on dysplastic dentin. Clin Oral Invest 23, 4335–4343 (2019). https://doi.org/10.1007/s00784-019-02863-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-019-02863-7

Keywords

Search

Navigation