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Amino Acids

, 36:417 | Cite as

Taurine plays a beneficial role against cadmium-induced oxidative renal dysfunction

  • Prasenjit Manna
  • Mahua Sinha
  • Parames C. SilEmail author
Original Article

Abstract

The present study has been carried out to investigate the role of taurine (2-aminoethanesulfonic acid), a conditionally essential amino acid, in ameliorating cadmium-induced renal dysfunctions in mice. Cadmium chloride (CdCl2) has been selected as the source of cadmium. Intraperitoneal administration of CdCl2 (at a dose of 4 mg/kg body weight for 3 days) caused significant accumulation of cadmium in renal tissues and lessened kidney weight to body weight ratio. Cadmium administration reduced intracellular ferric reducing/antioxidant power (FRAP) of renal tissues. Levels of serum marker enzymes related to renal damage, creatinine and urea nitrogen (UN) have been elevated due to cadmium toxicity. Cadmium exposure diminished the activities of enzymatic antioxidants, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and glucose-6-phosphate dehydrogenase (G6PD) as well as non-enzymatic antioxidant, reduced glutathione (GSH) and total thiols. On the other hand, the levels of oxidized glutathione (GSSG), lipid peroxidation, protein carbonylation, DNA fragmentation, concentration of superoxide radicals and activities of cytochrome P450 enzymes (CYP P450s) have been found to increase due to cadmium intoxication. Treatment with taurine (at a dose of 100 mg/kg body weight for 5 days) before cadmium intoxication prevented the toxin-induced oxidative impairments in renal tissues. The beneficial role of taurine against cadmium-induced renal damage was supported from histological examination of renal segments. Vitamin C, a well-established antioxidant was used as the positive control in the study. Experimental evidence suggests that both taurine and vitamin C provide antioxidant defense against cadmium-induced renal oxidative injury. Combining all, results suggest that taurine protects murine kidneys against cadmium-induced oxidative impairments, probably via its antioxidative property.

Keywords

Cadmium Oxidative impairment Renal dysfunction Taurine Antioxidant Renoprotective effect 

Abbreviations

BSA

Bovine serum albumin

CdCl2

Cadmium chloride

CDNB

1-Chloro-2,4-dinitrobenzene

DNPH

2,4-Dinitro phenyl hydrazine

DTNB

5,5′-Dithiobis(2-nitrobenzoic acid) [Ellman’s reagent]

EDTA

Ethylene diamine tetraacetic acid

FeCl3

Ferric chloride

FRAP

Ferric reducing/antioxidant power

GSH

Glutathione

GSSG

Glutathione disulfide

H2O2

Hydrogen peroxide

MDA

Malonaldehyde

NEM

N-ethylmaleimide

NADH

Nicotinamide adenine dinucleotide reduced disodium salt

NBT

Nitro blue tetrazolium chloride

PMT

Phenazine methosulphate

ROS

Reactive oxygen species

NaN3

Sodium azide

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

TPTZ

2,4,6-Tripyridyl-1,3,5-triazine

UN

Urea nitrogen

Notes

Acknowledgment

The authors are grateful to Mr. Prasanta Pal for excellent technical assistance for the study.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of ChemistryBose InstituteKolkataIndia

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