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The Inter/Intra-brain Metabolite Concentration Change as Applying the Rehabilitate Treatment in Intra-cerebral Hemorrhage Rat Models: Pilot Study

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Abstract

If intra-cerebral hemorrhage (ICH) rehabilitation animal model studies had included processes related to imaging diagnosis, not only interim evaluation of treatment effects but also the objectification of treatment effects would have been possible. The purpose of this study was to examine the rehabilitation treatment effect on biomarkers identified on magnetic resonance imaging/spectroscopy in an animal intra-cerebral hemorrhage model. Two groups of rats were used in this study: (1) the rehabilitation treatment group, 12 6-week-old Sprague–Dawley rats with experimental hemorrhage that received rehabilitation; and (2) the control group of 12 rats with experimental hemorrhage that received no intervention. Training rehabilitation was implemented 15 min daily for 2 weeks with 55–85% of the VO2 max. We conducted MRI/MRS scans before and after ICH rat modeling to evaluate brain metabolite concentration changes. The signal intensity of T2WI was measured at the site of ICH and in a similarly sized area on the opposite side. Integration of the areas under the peaks was conducted to measure cerebral metabolite concentrations. Differences in the mean T2WI-SI ratios measured 2 weeks after ICH induction were not statistically significant (p = 0.514) between the control group and the experimental group. However, the brain tCho/tCr metabolite ratio in the control group was significantly lower than in the experimental group 2 weeks after ICH induction (0.243 ± 0.044 vs. 0.326 ± 0.061, p = 0.007). The tCho/tCr ratio might be used as a biomarker to evaluate the effect of rehabilitation treatment for ICH.

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Acknowledgements

This work was supported by the Gimcheon University Research Grant.

Funding

This study was supported by Research Foundation of Korea (KR) (Grant Nos. 2018R1D1A1A02085800, 2015R1C1A1A02036988).

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Authors and Affiliations

  1. Department of Physical Therapy Science, College of Health Science, Gimcheon University, Gimcheon, 39528, Republic of Korea

    Sang-Hun Jang

  2. Department of Biomedical Laboratory Science, College of Health Science, Cheongju University, Cheongju, 28503, Republic of Korea

    Suk-Jun Lee

  3. Department of Radiological Science, College of Health Science, Gimcheon University, #214 Daehakro, Gimcheon, 39528, Republic of Korea

    Seung-Man Yu

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  1. Sang-Hun Jang
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Correspondence to Seung-Man Yu.

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Jang, SH., Lee, SJ. & Yu, SM. The Inter/Intra-brain Metabolite Concentration Change as Applying the Rehabilitate Treatment in Intra-cerebral Hemorrhage Rat Models: Pilot Study. Appl Magn Reson 50, 989–999 (2019). https://doi.org/10.1007/s00723-019-01132-w

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  • DOI: https://doi.org/10.1007/s00723-019-01132-w

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