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Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India

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Abstract

Association of a new begomovirus with leaf crumple disease of Jatropha curcas was identified based on sequence analysis of cloned ~2.7-kb viral DNA genomes from three representative samples amplified by RCA using phi-29 DNA polymerase. Sequence analysis of these isolates SKJ1 (KM066975), SKJ2 (KM189818) and SKJ3 (KM189819)—showed the presence of a monopartite begomovirus-like genome of 2,735 to 2,738 nucleotides containing seven ORFs: V3, V2 and V1 in virion sense and C3, C2, C1 and C4 in the complementary sense. Pairwise alignment showed 99 % nucleotide sequence similarity to each other and less than 81 % identity to other begomoviruses reported worldwide. Based on begomovirus species demarcation criteria for a new species (sequence identity <89 %), the begomovirus isolates were identified as the members of a new Begomovirus species and provisionally designated as Jatropha leaf crumple India virus (JLCrIV).

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Acknowledgments

The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, India, for facilities; the Department of Biotechnology, India, for funding; and to Dr. D. K. Mishra, Arid Forest Research Institute, Jodhpur, Rajasthan, India, for permission to collect samples.

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Authors and Affiliations

  1. Plant Molecular Virology Laboratory, CPMB Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, UP, India

    Ashish Srivastava, Meraj Jaidi, S. Kumar & S. K. Raj

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  1. Ashish Srivastava
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  2. Meraj Jaidi
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  3. S. Kumar
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  4. S. K. Raj
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Correspondence to S. K. Raj.

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Srivastava, A., Jaidi, M., Kumar, S. et al. Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India. Arch Virol 160, 617–619 (2015). https://doi.org/10.1007/s00705-014-2288-8

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  • DOI: https://doi.org/10.1007/s00705-014-2288-8

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