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A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas

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Abstract

Jatropha curcas mosaic disease is a newly emerging disease that challenges the productivity of a prospective biofuel crop, J. curcas. The aetiology of this disease has not been resolved. Here, we report the complete nucleotide sequences of a Jatropha virus isolated from Dharwad, Southern India. Phylogenetic analysis of the virus genome suggests it is a new strain of Indian cassava mosaic virus. Agroinfiltration of the two cloned viral DNA components produced systemic infection and typical mosaic symptoms in J. curcas, thereby fulfilling Koch’s postulates. The availability of infectious clones will provide a valuable tool to screen J. curcas cultivars for disease resistance and facilitate the generation of virus-resistant J. curcas plants by transgenic technology.

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Acknowledgments

We thank Drs. Srinivasan Ramachandran (Temasek Life Sciences Laboratory), Ramesh S. Bhat (University of Agricultural Sciences, Dharwad, Karnataka, India) and A. S. Byadgi (University of Agricultural Sciences, Dharwad, Karnataka, India) for their kind assistance in collecting J. curcas samples. We also thank the two anonymous reviewers whose helpful suggestions have led to an improvement of our manuscript. This work was supported by the Temasek Life Sciences Laboratory and the Singapore Millennium Foundation.

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Authors and Affiliations

  1. Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore

    ShiQiang Gao, Jing Qu & Jian Ye

  2. Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA

    Nam-Hai Chua

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  1. ShiQiang Gao
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  2. Jing Qu
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  3. Nam-Hai Chua
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  4. Jian Ye
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Correspondence to Jian Ye.

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Gao, S., Qu, J., Chua, NH. et al. A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas . Arch Virol 155, 607–612 (2010). https://doi.org/10.1007/s00705-010-0625-0

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  • DOI: https://doi.org/10.1007/s00705-010-0625-0

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