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Effects of growth medium, nutrients, water, and aeration on mycorrhization and biomass allocation of greenhouse-grown interior Douglas-fir seedlings

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Abstract

Commercial nursery practices usually fail to promote mycorrhization of interior Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. glauca (Beissn.) Franco] seedlings in British Columbia, which may account for their poor performance following planting in the field. We tested the effects of four nursery cultivation factors (nitrogen fertilization, phosphorus fertilization, watering, and soil aeration) and field soil addition on mycorrhization, survival, growth, and biomass allocation of interior Douglas-fir seedlings in a series of greenhouse experiments. Where field soil was added to the growing medium, mycorrhization and root/shoot ratios were maximized at lower levels of mineral nutrient application and aeration. Where field soil was not added, mycorrhization was negligible across all fertilization and aeration treatments, but root/shoot ratio was maximized at lower levels of mineral nutrients and the highest level of aeration. Regardless of whether field soil was added, intermediate levels of soil water resulted in the best mycorrhizal colonization and root/shoot ratios. However, field soil addition reduced seedling mortality at the two lowest water levels. A cluster analysis placed ectomycorrhizal morphotypes into three groups (Mycelium radicis-atrovirens Melin, Wilcoxina, and mixed) based on their treatment response, with all but two morphotypes in the mixed group whose abundance was maximized under conditions common to advanced seedling establishment. For maximal mycorrhization and root development of interior Douglas-fir seedlings, nurseries should minimize addition of nitrogen and phosphorus nutrients, maximize aeration, provide water at moderate rates, and, where possible, add small amounts of field soil to the growing medium.

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Authors and Affiliations

  1. Canadian Food Inspection Agency, Vancouver, BC, Canada

    Olga Kazantseva

  2. Department of Forest Sciences, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4

    Marcus Bingham & Suzanne W. Simard

  3. British Columbia Ministry of Forests & Range, Victoria, BC, Canada, V8W 9C4

    Shannon M. Berch

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  1. Olga Kazantseva
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  2. Marcus Bingham
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  3. Suzanne W. Simard
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  4. Shannon M. Berch
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Correspondence to Suzanne W. Simard.

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Kazantseva, O., Bingham, M., Simard, S.W. et al. Effects of growth medium, nutrients, water, and aeration on mycorrhization and biomass allocation of greenhouse-grown interior Douglas-fir seedlings. Mycorrhiza 20, 51–66 (2009). https://doi.org/10.1007/s00572-009-0263-0

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