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Redox potential as a key parameter for monitoring and optimization of xylose fermentation with yeast Spathaspora passalidarum under limited-oxygen conditions

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Abstract

The determination of optimum values of volumetric oxygen transfer coefficient (kLa) for Spathaspora passalidarum is an important aspect for the optimization of ethanol production from pentoses since oxygen plays a key role on yeast metabolism. By studying the fermentation of a xylose and glucose mixture, the highest ethanol volumetric productivity was achieved at a kLa of 45 h−1 (1.12 gethanol L−1 h−1), reducing the fermentation time to half when compared to other oxygen-limiting conditions that were considered optimum for other native strains, besides increasing xylose consumption rates. The high cell density fermentation showed to be a good strategy to be applied in industrial processes with S. passalidarum, enabling the complete exhaustion of a high initial substrate concentration (90 g L−1) in less than 24 h, with a final ethanol titer of 28.61 (± 0.42) g L−1. By performing a detailed investigation on oxidation–reduction potential (ORP), it was possible to conclude that the highest ethanol formation rates were registered at oxireduction potential values around − 100 mV, becoming an important parameter to be controlled when oxygen-limiting conditions are applied in industrial fermentations. The oxygen availability also affected the activity of enzyme XR and its preference for NADH or NADPH, directly affecting the activity of enzyme XDH and the redox imbalance on the xylose pathway. In addition, respirometric parameters were determined for the yeast S. passalidarum under an aerobic growth condition.

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Funding

The authors acknowledge the Brazilian Biorenewables National Laboratory—LNBR/CNPEM. This study was funded by FAPESP (Grant number 2016/06142-0, 2016/14567-0 and 2017/04997-0) and CNPq.

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Authors and Affiliations

  1. School of Chemical Engineering, State University of Campinas-UNICAMP, 500 Albert Einstein Av, Campinas, SP, 13083-852, Brazil

    Carolina I. D. G. Bonan, Luiz E. Biazi & Aline C. Costa

  2. Brazilian Biorenewables National Laboratory-LNBR/CNPEM, 10000 Giuseppe Maximo Scolfaro St, Campinas, SP, 13083-852, Brazil

    Carolina I. D. G. Bonan, Suzane R. Dionísio, Robson Tramontina, Amanda S. Sousa & Carlos A. de Oliveira Filho

  3. Chemical Engineering and Food Engineering Department, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil

    Lauren B. Soares & Jaciane L. Ienczak

  4. Institut of Biology, State University of Campinas-UNICAMP, 255 Monteiro Lobato, Campinas, SP, 13083-862, Brazil

    Robson Tramontina & Amanda S. Sousa

Authors
  1. Carolina I. D. G. Bonan
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  2. Luiz E. Biazi
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  3. Suzane R. Dionísio
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  4. Lauren B. Soares
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  7. Carlos A. de Oliveira Filho
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  8. Aline C. Costa
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  9. Jaciane L. Ienczak
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Corresponding author

Correspondence to Jaciane L. Ienczak.

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Conflict of interest

Carolina I.D.G. Bonan declares that she has no conflict of interest. Luiz E. Biazi declares that he has no conflict of interest. Suzane R. Dionísio declares that she has no conflict of interest. Lauren B. Soares declares that she has no conflict of interest. Robson Tramontina declares that he has no conflict of interest. Amanda S. Souza declares that she has no conflict of interest. Carlos A. de Oliveira Filho declares that he has no conflict of interest. Aline C. Costa declares that she has no conflict of interest. Jaciane L. Ienczak declares that she has no conflict of interest.

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Bonan, C.I.D.G., Biazi, L.E., Dionísio, S.R. et al. Redox potential as a key parameter for monitoring and optimization of xylose fermentation with yeast Spathaspora passalidarum under limited-oxygen conditions. Bioprocess Biosyst Eng 43, 1509–1519 (2020). https://doi.org/10.1007/s00449-020-02344-2

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