Abstract
In this work, the drastic change in the reaction rate throughout the acrylonitrile bio-hydration reaction, which was catalyzed by Rhodococcus ruber TH3 free cells in a two-liquid-phase system, was studied by changing the initial mass fraction of acrylonitrile and acrylamide. We found that the reaction rate was sensitively affected by the contact area between the acrylonitrile droplets and cells. With the acrylonitrile mass fraction of 3 wt%, the cell solution of 800 U/mL could make the superficial area of acrylonitrile droplets saturated. The sustained increase of the acrylamide concentration in the reaction process could reduce the reaction rate, and 25 wt% was the obvious inflection point. The interface adsorption of cells was visually observed with the method of fluorescence microscopy, and the uptake mechanism of substrate by direct contact was illustrated to play a main role by comparing the reaction rate of the heterogeneous system and that of the homogeneous system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wei W, Li A, Yang J, Ma F, Wu D, Xing J, Zhou X, Zhao D (2015) Synergetic effects and flocculation behavior of anionic polyacrylamide and extracellular polymeric substrates extracted from Klebsiella sp. J1 on improving soluble cadmium removal. Bioresour Technol 175:34–41
Wang Q, Liu S, Yang G, Chen J, Ni Y (2015) Cationic polyacrylamide enhancing cellulase treatment efficiency of hardwood kraft-based dissolving pulp. Bioresour Technol 183:42–46
Thanyacharoen U, Tani A, Charoenpanich J (2012) Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation. J Environ Sci Health A Tox Hazard Subst Environ Eng 47:1491–1499
Feng Y-S, Chen P-C, Wen F-S, Hsiao W-Y, Lee C-M (2008) Nitrile hydratase from Mesorhizobium sp. F28 and its potential for nitrile biotransformation. Process Biochem 43:1391–1397
Ma Y, Yu H (2012) Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition. J Ind Microbiol Biotechnol 39:1421–1430
Chen J, Yu H, Liu C, Liu J, Shen Z (2012) Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions. J Biotechnol 164:354–362
Sakashita T, Hashimoto Y, Oinuma K, Kobayashi M (2008) Transcriptional regulation of the nitrile hydratase gene cluster in Pseudomonas chlororaphis B23. J bacteriol 190:4210–4217
Zhang J, Pierce GE (2009) Laboratory-scale biofiltration of acrylonitrile by Rhodococcus rhodochrous DAP 96622 in a trickling bed bioreactor. J Ind Microbiol Biotechnol 36:971–979
Wilson L, Betancor L, Fernandez-Lorente G, Fuentes M, Hidalgo A, Guisan JM, Pessela BCC, Fernandez-Lafuente R (2004) Cross-linked aggregates of multimeric enzymes: a simple and efficient methodology to stabilize their quaternary structure. Bio Macromol 5:814–817
Alexopoulos A, Maggioris D, Kiparissides C (2002) CFD analysis of turbulence non-homogeneity in mixing vessels at two-compartment model. Chem Eng Sci 57:1735–1752
Sun X, Yu H, Shen Z (2009) Deactivation kinetics of nitrile hydratase in free resting cells. Chin J Chem Eng 17:822–828
Li J, Guo M, Jiao S, Wang Y, Luo G, Yu H (2017) A kinetic study of the biological catalytic hydration of acrylonitrile to acrylamide. Chem Eng Sci 317:699–706
Watanabe H, Tanji Y, Unno H, Hori K (2008) Rapid conversion of toluene by an acinetobacter sp. Tol 5 mutant showing monolayer adsorption to water-oil interface. J Biosci Bioeng 106:226–230
Ma Y, Yu H, Pan W, Liu C, Zhang S, Shen Z (2010) Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant. Bioresour Technol 101:285–291
Li J, Yang L, Ding X, Chen J, Wang Y, Luo G, Yu H (2015) Visual study of mass transfer characterization in the process of biological catalytic hydration of acrylonitrile using pendant drop method. RSC Adv 5:79164–79171
Li J, Chen Y, Guo M, Wang Y, Xu J, Luo G, Yu H (2016) Using microchannels to visually investigate the formation and dissolution of acrylonitrile droplets in a bio-hydration system. Chem Eng Sci 152:239–247
Li J, Liu J, Chen J, Wang Y, Luo G, Yu H (2015) Multiple reuses of Rhodococcus ruber TH3 free cells to produce acrylamide in a membrane dispersion microreactor. Bioresour Technol 187:198–204
Acknowledgements
This work was financially supported by the National Basic Research Foundation of China (Grant No. 2013CB733600), the National Natural Science Foundation (Grant Nos. 21276140 and 21036002), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130002110020).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Guo, M., Yang, L., Li, J. et al. Effects of interface adsorption of Rhodococcus ruber TH3 cells on the biocatalytic hydration of acrylonitrile to acrylamide. Bioprocess Biosyst Eng 41, 931–938 (2018). https://doi.org/10.1007/s00449-018-1924-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-018-1924-1