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Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6

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Abstract

Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with α-, β- and δ-crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN’s status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family.

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Acknowledgements

We are grateful to Drs. B. Friday and A. Leddoz for inspiring discussions as well as for the valuable input of the reviewers.

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  1. Gabriel García Caballero and Sebastian Schmidt contributed equally to this work.

Authors and Affiliations

  1. Institute for Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539, Munich, Germany

    Gabriel García Caballero, Sebastian Schmidt, Anna-Kristin Ludwig, Joachim C. Manning, Herbert Kaltner & Hans-Joachim Gabius

  2. Functional Proteome Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120, Heidelberg, Germany

    Martina Schnölzer

  3. Department of Ophthalmology, Friedrich-Alexander-University Erlangen-Nuremberg, Schwabachanlage 6, 91054, Erlangen, Germany

    Ursula Schlötzer-Schrehardt

  4. Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539, Munich, Germany

    Clemens Knospe

  5. Promega GmbH, Schildkrötstr. 15, 68199, Mannheim, Germany

    Paul Muschler

  6. Department of Applied Tumor Biology, Institute of Pathology, Medical School of the Ruprecht-Karls-University Heidelberg, Im Neuenheimer Feld 224, 69120, Heidelberg, Germany

    Jürgen Kopitz

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  1. Gabriel García Caballero
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García Caballero, G., Schmidt, S., Schnölzer, M. et al. Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6. Cell Tissue Res 375, 665–683 (2019). https://doi.org/10.1007/s00441-018-2931-x

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