Skip to main content

Advertisement

SpringerLink
Log in

Human keratinocyte caspase-14 expression is altered in human epidermal 3D models by dexamethasone and by natural products used in cosmetics

  • Original Paper
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Abstract

Caspase-14 is a cysteinyl-aspartate-specific proteinase that is specifically expressed in epidermal keratinocytes. Dysregulation of caspase-14 expression is implicated in impaired skin barrier formation. To elucidate the regulation of caspase-14 in differentiated keratinocytes, we characterized the expression of caspase-14 in normal human epidermal keratinocytes (NHEKs) and two types of three-dimensional (3D) human epidermis culture models, EPI-200 and EPI-201, via RT-PCR and immunoblot analyses. Caspase-14 expression was absent in subconfluent NHEKs, but was present in confluent NHEKs as well as those induced to differentiate by calcium. Caspase-14 expression levels in the 3D epidermis models were almost equal to that in the Ca2+-treated differentiated NHEKs. Despite the presence of caspase-14 expression in these models, caspase-14 activity was found only in the mature 3D skin model, EPI-200. This was confirmed by detection of a 17 kDa cleaved fragment of caspase-14 present only in the EPI-200 model. Since glucocorticoid (GC) receptor is required for skin barrier competence, we investigated whether the GC dexamethasone (Dex) and various natural components of common skin moisturizers affect caspase-14 expression in keratinocytes. Dex decreased caspase-14 expression in undifferentiated, but not differentiated, NHEKs. Conversely, Dex increased caspase-14 expression in both 3D skin models, although it did not alter caspase protease activity. Similar to treatment with Dex, treatment of the premature 3D skin mode, EPI-201 with a Galactomyces ferment filtrate markedly increased expression of caspase-14. Further, these results suggest that the effect of Dex, or lack thereof, on caspase-14 expression is dependent on the stage of keratinocyte differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Alibardi L, Tschachler E, Eckhart L (2005) Distribution of caspase-14 in epidermis and hair follicles is evolutionarily conserved among mammals. Anat Rec A Discov Mol Cell Evol Biol 286:962–973

    PubMed  Google Scholar 

  2. Ballaun C, Karner S, Mrass P, Mildner M, Buchberger M, Bach J, Ban J, Harant H, Tschachler E, Eckhart L (2008) Transcription of the caspase-14 gene in human epidermal keratinocytes requires AP-1 and NFkappaB. Biochem Biophys Res Commun 371:261–266

    Article  PubMed  CAS  Google Scholar 

  3. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, Pérez P (2008) Glucocorticoid receptor is required for skin barrier competence. Endocrinology 149:1377–1388

    Article  PubMed  CAS  Google Scholar 

  4. Candi E, Schmidt R, Melino G (2005) The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol 6:328–340

    Article  PubMed  CAS  Google Scholar 

  5. Chien AJ, Presland RB, Kuechle MK (2002) Processing of native caspase-14 occurs at an atypical cleavage site in normal epidermal differentiation. Biochem Biophys Res Commun 296(4):911–917

    Article  PubMed  CAS  Google Scholar 

  6. Denecker G, Hoste E, Gilbert B, Hochepied T, Ovaere P, Lippens S, Van den Broecke C, Van Damme P, D’Herde K, Hachem JP, Borgonie G, Presland RB, Schoonjans L, Libert C, Vandekerckhove J, Gevaert K, Vandenabeele P, Declercq W (2007) Caspase-14 protects against epidermal UVB photodamage and water loss. Nat Cell Biol 9:666–674

    Article  PubMed  CAS  Google Scholar 

  7. Denecker G, Ovaere P, Vandenabeele P, Declercq W (2008) Caspase-14 reveals its secrets. J Cell Biol 180:451–458

    Article  PubMed  CAS  Google Scholar 

  8. Eckhart L, Declercq W, Ban J, Rendl M, Lengauer B, Mayer C, Lippens S, Vandenabeele P, Tschachler E (2000) Terminal differentiation of human keratinocytes and stratum corneum formation is associated with caspase-14 activation. J Invest Dermatol 115:1148–1151

    Article  PubMed  CAS  Google Scholar 

  9. Fischer H, Stichenwirth M, Dockal M, Ghannadan M, Buchberger M, Bach J, Kapetanopoulos A, Declercq W, Tschachler E, Eckhart L (2004) Stratum corneum-derived caspase-14 is catalytically active. FEBS Lett 577:446–450

    Article  PubMed  CAS  Google Scholar 

  10. Hibino T, Fujita E, Tsuji Y, Nakanishi J, Iwaki H, Katagiri C, Momoi T (2010) Purification and characterization of active caspase-14 from human epidermis and development of the cleavage site-directed antibody. J Cell Biochem 109:487–497

    PubMed  CAS  Google Scholar 

  11. Hoste E, Kemperman P, Devos M, Denecker G, Kezic S, Yau N, Gilbert B, Lippens S, De Groote P, Roelandt R, Van Damme P, Gevaert K, Presland RB, Takahara H, Puppels G, Caspers P, Vandenabeele P, Declercq W (2011) Caspase-14 is required for filaggrin degradation to natural moisturizing factors in the skin. J Invest Dermatol 131:2233–2241

    Article  PubMed  CAS  Google Scholar 

  12. Hsu S, Dickinson D, Borke J, Walsh DS, Wood J, Qin H, Winger J, Pearl H, Schuster G, Bollag WB (2007) Geen tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model. Exp Dermatol 16:678–684

    Article  PubMed  CAS  Google Scholar 

  13. Kushida A, Hattori K, Yamaguchi N, Kobayashi T, Date A, Tamura H (2011) Sulfation of estradiol in human epidermal keratinocyte. Biol Pharm Bull 34:1147–1151

    Article  PubMed  CAS  Google Scholar 

  14. Lippens S, Kockx M, Denecker G, Knaapen M, Verheyen A, Christiaen R, Tschachler E, Vandenabeele P, Declercq W (2004) Vitamin D3 induces caspase-14 expression in psoriatic lesions and enhances caspase-14 processing in organotypic skin cultures. Am J Pathol 165:8338–8341

    Article  Google Scholar 

  15. Poumay Y, Dupont F, Marcoux S, Leclercq-Smekens M, Hérin M, Coquette A (2004) A simple reconstructed human epidermis: preparation of the culture model and utilization in vitro studies. Arch Dermatol Res 296:203–211

    Article  PubMed  CAS  Google Scholar 

  16. Rawlings AV, Voegeli R (2012) Stratum corneum proteases and dry skin conditions. Cell Tissue Res (Epub ahead of print)

  17. Rendl M, Ban J, Mrass P, Mayer C, Lengauer B, Eckhart L, Declerq W, Tschachler E (2002) Caspase-14 expression by epidermal keratinocytes is regulated by retinoids in a differentiation-associated manner. J Invest Dermatol 119:1150–1155

    Article  PubMed  CAS  Google Scholar 

  18. Schäcke H, Döcke WD, Asadullah K (2002) Mechanisms involved in the side effects of glucocorticoids. Pharmacol Ther 96:23–43

    Article  PubMed  Google Scholar 

  19. Vital AL, Gonçalo M, Cruz MT, Figueiredo A, Duarte CB, Lopes MC (2003) Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-kappaB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line. Mediat Inflamm 12:71–78

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by a grant from the Promotion and Mutual Aid Corporation for Private Schools of Japan. This work was supported by a fund from Procter and Gamble Japan.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Keio University, 1-5-30, Shibakoen, Minatoku, Tokyo, 105-8512, Japan

    Saori Kataoka, Kenji Hattori & Hiroomi Tamura

  2. School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe-shi, Hyogo, 650-8530, Japan

    Akira Date

Authors
  1. Saori Kataoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kenji Hattori
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Date
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroomi Tamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroomi Tamura.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kataoka, S., Hattori, K., Date, A. et al. Human keratinocyte caspase-14 expression is altered in human epidermal 3D models by dexamethasone and by natural products used in cosmetics. Arch Dermatol Res 305, 683–689 (2013). https://doi.org/10.1007/s00403-013-1359-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00403-013-1359-0

Keywords

Search

Navigation