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Interactions of human tenascin-X domains with dermal extracellular matrix molecules

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Abstract

Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. Deficiency of TNX causes a recessive form of Ehlers–Danlos syndrome characterized by joint hypermobility, skin fragility and hyperextensible skin. Skin of TNX deficient patients shows abnormal elastic fibers and reduced collagen deposition. The mechanism by which TNX deficiency leads to connective tissue alterations is unknown. Here we report that C-terminal domains of human TNX bind to major dermal fibrillar collagens and tropoelastin. We have mapped these interactions to the fibronectin type III repeat 29 (FNIII29) and the C-terminal fibrinogen domain (FbgX) of TNX. In addition we found that FNIII29 of TNX accelerates collagen fibrillogenesis in vitro. We hypothesize that TNX contributes to matrix stability and is possibly involved in collagen fibril formation.

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Acknowledgments

The authors are grateful to Dr P. Handford, Dr N. Grebenchtchikov and Dr R. Mecham for providing biological reagents used in this study.

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Authors and Affiliations

  1. Department of Dermatology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    David Egging, Franka van den Berkmortel & Joost Schalkwijk

  2. Department of Pediatrics, University of California, San Francisco, CA, USA

    Glen Taylor & Jim Bristow

  3. Department of Genome Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

    Jim Bristow

Authors
  1. David Egging
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  2. Franka van den Berkmortel
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  3. Glen Taylor
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  4. Jim Bristow
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  5. Joost Schalkwijk
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Correspondence to David Egging.

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Egging, D., van den Berkmortel, F., Taylor, G. et al. Interactions of human tenascin-X domains with dermal extracellular matrix molecules. Arch Dermatol Res 298, 389–396 (2007). https://doi.org/10.1007/s00403-006-0706-9

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  • DOI: https://doi.org/10.1007/s00403-006-0706-9

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