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Biosutures improve healing of experimental weak colonic anastomoses

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Abstract

Purpose

Use of biosutures in animal models of colonic anastomoses is associated with decreased adhesions without affecting anastomotic strength. This study aimed to evaluate the effect of biosutures on colonic anastomoses kept free of adhesions by peritoneal instillation of icodextrin 4%.

Methods

Three types of colonic anastomoses were compared: group 1, anastomoses without icodextrin 4% and control suture; group 2, anastomoses with icodextrin 4% (adhesion-free anastomoses) and control suture; and group 3, anastomoses with icodextrin 4% (adhesion-free anastomoses) and biosutures. Dehiscence, adhesion formation, and anastomotic strength were evaluated on day 4 after the operation.

Results

When peritoneal icodextrin 4% was used in anastomoses with conventional sutures, a decrease in the adhesion index (P = 0.01) and a lower bursting pressure (P = 0.15) were observed. When adhesion-free anastomoses were compared, those performed with biosutures had a higher bursting pressure (P = 0.008) and a similar pattern of adhesion index (P = 0.48).

Conclusions

Biosutures improve the strength of adhesion-free colonic anastomoses.

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References

  1. Nasirkhan MU, Abir F, Longo W et al (2006) Anastomotic disruption after large bowel resection. World J Gastroenterol 12:2497–2504

    PubMed  Google Scholar 

  2. García-Olmo D, García-Arranz M, Herreros D et al (2005) A phase I clinical trial of the treatment of Crohn's fistula by adipose mesenchymal stem cell transplantation. Dis Colon Rectum 48:1416–1423

    Article  PubMed  Google Scholar 

  3. García-Olmo D, García-Arranz M, García LG et al (2003) Autologous stem cell transplantation for treatment of rectovaginal fistula in perianal Crohn's disease: a new cell-based therapy. Int J Colorectal Dis 18:451–454

    Article  PubMed  Google Scholar 

  4. Garcia-Olmo D, Herreros D, Pascual I et al (2009) Expanded adipose-derived stem cells (CX401) for the treatment of complex perianal fistula. A phase II clinical trial. Dis Colon Rectum 52:79–86

    PubMed  Google Scholar 

  5. Pascual I, Fernández de Miguel G, Gómez-Pinedo UA et al (2008) Adipose-derived mesenchymal stem cells in biosutures do not improve healing of experimental colonic anastomoses. Br J Surg 95:1180–1184

    Article  CAS  PubMed  Google Scholar 

  6. García-Olmo D, Lucas FJ, Paya J (1996) Relationship between peritoneum adhesion phenomena and the experimental resistance of colon anastomoses: influence of omentoplasty. Eur Surg Res 28:315–322

    Article  PubMed  Google Scholar 

  7. Tang CL, Jayne DG, Seow-Choen F et al (2006) A randomized controlled trial of 0.5% ferric hyaluronate gel (Intergel) in the prevention of adhesions following abdominal surgery. Ann Surg 243:449–455

    Article  PubMed  Google Scholar 

  8. Beck DE, Cohen Z, Fleshman JW et al (2003) A prospective, randomized, multicenter, controlled study of the safety of seprafilm adhesion barrier in abdominopelvic surgery of the intestine. Dis Colon Rectum 46:1310–1319

    Article  PubMed  Google Scholar 

  9. Menzies D, Pascual MH, Walz MK et al (2006) Use of icodextrin 4% solution in the prevention of adhesion formation following general surgery: from the multicentre ARIEL Registry. Ann R Coll Surg Engl 88:375–382

    Article  CAS  PubMed  Google Scholar 

  10. Wang Y, Kim HJ, Vunjak-Novakovic G et al (2006) Stem cell-based tissue engineering with silk biomaterials. Biomaterials 27:6064–6082

    Article  CAS  PubMed  Google Scholar 

  11. Wei HJ, Chen CH, Lee WY et al (2008) Bioengineered cardiac patch constructed from multilayered mesenchymal stem cells for myocardial repair. Biomaterials 29:3547–3556

    Article  CAS  PubMed  Google Scholar 

  12. Baca B, Boler DE, Onur E (2007) Icodextrin and seprafilm do not interfere with colonic anastomosis in rats. Eur Surg Res 39:318–323

    Article  CAS  PubMed  Google Scholar 

  13. Rodgers KE, Verco SJ, diZerega GS (2003) Effects of intraperitoneal 4% icodextrin solution on the healing of bowel anastomoses and laparotomy incisions in rabbits. Colorectal Dis 5:324–330

    Article  CAS  PubMed  Google Scholar 

  14. Hendriks T, Mastboom WJ (1990) Healing of experimental intestinal anastomoses. Parameters for repair. Dis Colon Rectum 33:891–901

    Article  CAS  PubMed  Google Scholar 

  15. Willis S, Hölzl F, Krones CJ et al (2006) Evaluation of anastomotic microcirculation after low anterior rectal resection: an experimental study with different reconstruction forms in dogs. Tech Coloproctol 10:222–226

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery C, La Paz University Hospital, Paseo de la Castellana 261, 28046, Madrid, Spain

    Isabel Pascual & Damián García-Olmo

  2. Cellerix, S.L., Madrid, Spain

    Gemma Fernández de Miguel

  3. Cell Therapy Unit, La Paz University Hospital, Madrid, Spain

    Mariano García Arranz & Damián García-Olmo

  4. Department of Surgery, Autonoma University of Madrid, Madrid, Spain

    Damián García-Olmo

Authors
  1. Isabel Pascual
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  2. Gemma Fernández de Miguel
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  3. Mariano García Arranz
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  4. Damián García-Olmo
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Corresponding author

Correspondence to Isabel Pascual.

Additional information

This study was supported in part by funds from Cellerix S.L. to the Chair of Cell Therapy and Regenerative Medicine “Universidad Autonoma de Madrid–Cellerix” and by a grant from the Spanish Ministry of Health and Consumer Affairs (FIS PI060305, PI060292, and RETIC Program RD06/0010/0018) and Bioscience Program of the Community of Madrid (S-BIO-0204/2006-MESENCAM).

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Pascual, I., Fernández de Miguel, G., García Arranz, M. et al. Biosutures improve healing of experimental weak colonic anastomoses. Int J Colorectal Dis 25, 1447–1451 (2010). https://doi.org/10.1007/s00384-010-0952-3

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  • DOI: https://doi.org/10.1007/s00384-010-0952-3

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