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Isolation of Viable but Non-culturable Bacteria from Printing and Dyeing Wastewater Bioreactor Based on Resuscitation Promoting Factor

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Abstract

Printing and dyeing wastewater with high content of organic matters, high colority, and poor biochemical performance is hard to be degraded. In this study, we isolated viable but non-culturable (VBNC) bacteria from printing and dyeing wastewater with the culture media contained resuscitation promoting factor (Rpf) protein secreted by Micrococcus luteus, counted the culturable cells number with the most probable number, sequenced 16S rRNA genes, and performed polymerase chain reaction-denaturing gradient gel electrophoresis. It is obviously that the addition of Rpf in the enrichment culture could promote growth and resuscitation of bacteria in VBNC state to obtain more fastidious bacteria significantly. The identified bacteria were assigned to nine genera in the treatment group, while the two strains of Ochrobactrum anthropi and Microbacterium sp. could not be isolated from the control group. The function of isolated strains was explored and these strains could degrade the dye of Congo red. This study provides a new sight into the further study including the present state, composition, formation mechanism, and recovery mechanism about VBNC bacteria in printing and dyeing wastewater, which would promote to understand bacterial community in printing and dyeing wastewater, and to obtain VBNC bacteria from ecological environment.

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Acknowledgements

This research was supported by the Zhejiang Provincial Public Welfare Technology Applied Research Project (2017C33046), Zhejiang Provincial Natural Science Foundation of China (LY13C010002), Shenzhen Basic Research Project (JCYJ20140417113430641, JCYJ20140509174140691 and JCYJ20140417113430732), Foundation of Shenzhen Strategic Emerging Industries Development (JSKF20150831171545604) and Shenzhen Technology development Project (CXZZ20150529144041624).

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Authors and Affiliations

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Yingbin Road 688#, Jinhua, 321004, People’s Republic of China

    Yi Jin, Xiaoyun Yu & Linxian Ding

  2. Laboratory for Food Safety and Environmental Technology, ShenZhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People’s Republic of China

    Guojuan Gan, Xiaoqing Yan, Yan Liang, Linxian Ding & Yonglong Pan

  3. College of Geography and Environmental Sciences, Zhejiang Normal University, Yingbin Road 688#, Jinhua, 321004, People’s Republic of China

    Dongdong Wu, Jiadan Hu, Huachang Hong & Linxian Ding

  4. Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Li Zhang, Na Yang, Zhiheng Liu & Lixin Zhang

  5. University of Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Li Zhang & Na Yang

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  1. Yi Jin
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  2. Guojuan Gan
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  13. Linxian Ding
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Correspondence to Linxian Ding or Yonglong Pan.

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Jin, Y., Gan, G., Yu, X. et al. Isolation of Viable but Non-culturable Bacteria from Printing and Dyeing Wastewater Bioreactor Based on Resuscitation Promoting Factor. Curr Microbiol 74, 787–797 (2017). https://doi.org/10.1007/s00284-017-1240-z

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  • DOI: https://doi.org/10.1007/s00284-017-1240-z

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