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Complete mitochondrial genome of the endophytic fungus Pestalotiopsis fici: features and evolution

  • Genomics, transcriptomics, proteomics
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Abstract

Endophytic fungi (EF) live within plants and have profound impacts on plant communities. They are astonishingly diverse but poorly studied at the genome level. Herein, we assembled the mitochondrial genome (mitogenome) of the EF Pestalotiopsis fici, annotated and compared it with those of other relatives to better understand the evolution of the EF lineage. Except for standard fungal mitochondrial genes, the 69,529-bp circular mitogenome of P. fici harbors 18 introns acquired possibly through lateral transfer from other fungi and nine free-standing open reading frames with some scarcely seen in fungal mitogenomes. BLAST analysis detected no obvious duplication events of large fragments between mitochondrial and nuclear genomes of the fungus. Transcription analyses validated the expression of all mitochondrial genes, while most genes showed higher expression on rice than in two other media. The mitogenome of P. fici is highly syntenic with the Xylariales species Annulohypoxylon stygium and the endophyte Epichloe festucae var. lolii, but lacks synteny with another endophyte Penicillium polonicum. This study reports the first mitogenome of Pestalotiopsis and the third published mitogenome from an EF and provides insights into the evolution of the EF lineage.

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Acknowledgements

This study was funded by the National Science Foundation of China (81102759), the Natural Science Foundation of Shanxi Province (2014021030-2, 201601D011065), the Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province, and the Special Fund for Large Scientific Instruments and Equipments in Shanxi Province. The authors thank Xiaoqing Yang for her help in drawing the circular mitochondrial map.

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Authors and Affiliations

  1. Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China

    Shu Zhang

  2. Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Xiu-Na Wang

  3. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Xiao-Ling Zhang & Xing-Zhong Liu

  4. School of Life Sciences, Shanxi University, Taiyuan, 030006, China

    Yong-Jie Zhang

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  1. Shu Zhang
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Correspondence to Yong-Jie Zhang.

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Zhang, S., Wang, XN., Zhang, XL. et al. Complete mitochondrial genome of the endophytic fungus Pestalotiopsis fici: features and evolution. Appl Microbiol Biotechnol 101, 1593–1604 (2017). https://doi.org/10.1007/s00253-017-8112-0

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  • DOI: https://doi.org/10.1007/s00253-017-8112-0

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