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Insights into the Phylogeny or Arylamine N-Acetyltransferases in Fungi

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Abstract

Previous studies have shown that Eumycetes fungi can acylate arylamine thanks to arylamine N-acetyltransferases, xenobiotic-metabolizing enzymes also found in animals and bacteria. In this article, we present the results of mining 96 available fungal genome sequences for arylamine N-acetyltransferase genes and propose their phylogeny. The filamentous Pezizomycotina are shown to possess many putative N-acetyltransferases, whilst these are often lacking in other fungal groups. The evolution of the N-acetyltransferases is best explained by the presence of at least one gene in the opisthokont ancestor of the fungi and animal kingdoms, followed by recurrent gene losses and gene duplications. A possible horizontal gene transfer event may have occurred from bacteria to the basidiomycetous yeast Malassezia globosa.

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Acknowledgments

We thank Roger Townsend for correcting the manuscript and three anonymous reviewers for their helpful comments.

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  1. Marta Martins

    Present address: Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, Fulham Road, London, SW3 6JB, UK

Authors and Affiliations

  1. Univ Paris Diderot-Paris 7, Unité de Biologie Fonctionnelle et Adaptative (BFA), CNRS EAC 4413, Laboratoire des Réponses Moléculaires et Cellulaires aux Xénobiotiques, 75013, Paris, France

    Marta Martins, Julien Dairou, Fernando Rodrigues-Lima & Jean-Marie Dupret

  2. UFR des Sciences du Vivant, Univ Paris Diderot-Paris 7, 75013, Paris, France

    Marta Martins, Julien Dairou, Fernando Rodrigues-Lima, Jean-Marie Dupret & Philippe Silar

  3. Institut de Génétique et Microbiologie, CNRS UMR 8621, Univ Paris-Sud, 91405, Orsay Cedex, France

    Philippe Silar

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Correspondence to Philippe Silar.

Electronic Supplementary Material

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239_2010_9371_MOESM1_ESM.rtf

Supplementary Fig. 1 online: The alignment of fungal, animal and bacterial NAT was made with MUSCLE (Edgar 2004). Conserved amino acids are highlighted in black, similar ones in grey. The NAT motifs are underlined, and the Cys-His-Asp catalytic triad is indicated by black dots. Intron positions are indicated by the vertical red bars. Position of introns I1 and I2 of Pezizomycotina is indicated underneath the alignment. (RTF 1724 kb)

239_2010_9371_MOESM2_ESM.tif

Supplementary Fig. 2 online: Tree generated by Neighbour Joining from the alignment of Supplementary Fig. 1 online. NAT families I–VII are from Pezizomycotina. Arrow point towards the M. globosa NAT gene. (TIFF 894 kb)

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Martins, M., Dairou, J., Rodrigues-Lima, F. et al. Insights into the Phylogeny or Arylamine N-Acetyltransferases in Fungi. J Mol Evol 71, 141–152 (2010). https://doi.org/10.1007/s00239-010-9371-x

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