Applied Microbiology and Biotechnology

, Volume 100, Issue 12, pp 5437–5452

Genetic dissection of fruiting body-related traits using quantitative trait loci mapping in Lentinula edodes

  • Wen-bing Gong
  • Lei Li
  • Yan Zhou
  • Yin-bing Bian
  • Hoi-shan Kwan
  • Man-kit Cheung
  • Yang Xiao
Applied genetics and molecular biotechnology

DOI: 10.1007/s00253-016-7347-5

Cite this article as:
Gong, W., Li, L., Zhou, Y. et al. Appl Microbiol Biotechnol (2016) 100: 5437. doi:10.1007/s00253-016-7347-5

Abstract

To provide a better understanding of the genetic architecture of fruiting body formation of Lentinula edodes, quantitative trait loci (QTLs) mapping was employed to uncover the loci underlying seven fruiting body-related traits (FBRTs). An improved L. edodes genetic linkage map, comprising 572 markers on 12 linkage groups with a total map length of 983.7 cM, was constructed by integrating 82 genomic sequence-based insertion-deletion (InDel) markers into a previously published map. We then detected a total of 62 QTLs for seven target traits across two segregating testcross populations, with individual QTLs contributing 5.5 %–30.2 % of the phenotypic variation. Fifty-three out of the 62 QTLs were clustered in six QTL hotspots, suggesting the existence of main genomic regions regulating the morphological characteristics of fruiting bodies in L. edodes. A stable QTL hotspot on MLG2, containing QTLs for all investigated traits, was identified in both testcross populations. QTLs for related traits were frequently co-located on the linkage groups, demonstrating the genetic basis for phenotypic correlation of traits. Meta-QTL (mQTL) analysis was performed and identified 16 mQTLs with refined positions and narrow confidence intervals (CIs). Nine genes, including those encoding MAP kinase, blue-light photoreceptor, riboflavin-aldehyde-forming enzyme and cyclopropane-fatty-acyl-phospholipid synthase, and cytochrome P450s, were likely to be candidate genes controlling the shape of fruiting bodies. The study has improved our understanding of the genetic architecture of fruiting body formation in L. edodes. To our knowledge, this is the first genome-wide QTL detection of FBRTs in L. edodes. The improved genetic map, InDel markers and QTL hotspot regions revealed here will assist considerably in the conduct of future genetic and breeding studies of L. edodes.

Keywords

Shiitake mushroom Fruiting body-related traits QTL co-localisation QTL hotspots Candidate genes 

Supplementary material

253_2016_7347_MOESM1_ESM.pdf (188 kb)
ESM 1(PDF 188 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Wen-bing Gong
    • 1
    • 2
  • Lei Li
    • 3
  • Yan Zhou
    • 1
  • Yin-bing Bian
    • 1
  • Hoi-shan Kwan
    • 3
  • Man-kit Cheung
    • 3
  • Yang Xiao
    • 1
  1. 1.Institute of Applied MycologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Institute of Bast Fiber CropsChinese Academy of Agricultural SciencesChangshaPeople’s Republic of China
  3. 3.School of Life SciencesThe Chinese University of Hong KongHong Kong SARPeople’s Republic of China

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