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Diversity and abundance of anammox bacterial community in the deep-ocean surface sediment from equatorial Pacific

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Abstract

The community structure and diversity of anaerobic ammonium oxidation (anammox) bacteria in the surface sediments of equatorial Pacific were investigated by phylogenic analysis of 16S rRNA and hydrazine oxidoreductase (hzo) genes and PCoA (principal coordinates analysis) statistical analysis. Results indicated that 16S rRNA and hzo sequences in the P2 (off the center of western Pacific warm pool) and P3 (in the eastern equatorial Pacific) sites all belong to the Candidatus “Scalindua”, the dominate anammox bacteria in the low-temperature marine environment proved by previous studies. However, in the P1 site (in center of warm pool of western Pacific), large part of 16S rRNA gene sequences formed a separated cluster. Meanwhile, hzo gene sequences from P1 sediment also grouped into a single cluster. PCoA analysis demonstrated that the anammox community structure in the P1 has significant geographical distributional difference from that of P2, P3, and other marine environments based on 16S rRNA and hzo genes. The abundances of anammox bacteria in surface sediments of equatorial Pacific were quantified by q-PCR analysis of hzo genes, which ranged from 3.98 × 103 to 1.17 × 104 copies g−1 dry sediments. These results suggested that a special anammox bacteria phylotypes exist in the surface sediment of the western Pacific warm pool, which adapted to the specific habitat and maybe involved in the nitrogen loss process from the fixed inventory in the habitat.

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Acknowledgments

This research was supported by Knowledge Innovation Key Project of The Chinese Academy of Sciences (KZCX2-YW-QN207); National Natural Science Foundation of China (No.30800032, 41076095, and 40930847) and MEL Advanced Visiting Scholarship (MELRS0911).

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  1. Key Laboratory of Tropical Marine Environment Dynamics (LED), South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou, 510301, China

    Yi-Guo Hong & Bo Yin

  2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China

    Tian-Ling Zheng

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  1. Yi-Guo Hong
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  2. Bo Yin
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Correspondence to Yi-Guo Hong.

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Hong, YG., Yin, B. & Zheng, TL. Diversity and abundance of anammox bacterial community in the deep-ocean surface sediment from equatorial Pacific. Appl Microbiol Biotechnol 89, 1233–1241 (2011). https://doi.org/10.1007/s00253-010-2925-4

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