Applied Microbiology and Biotechnology

, Volume 71, Issue 4, pp 555–562 | Cite as

Long-term performance and microbial dynamics of an up-flow fixed bed reactor established for the biodegradation of fluorobenzene

  • M. F. Carvalho
  • R. Ferreira Jorge
  • C. C. Pacheco
  • P. De Marco
  • I. S. Henriques
  • A. Correia
  • P. M. L. Castro
Environmental biotechnology


An up-flow fixed bed reactor (UFBR) was established to investigate the biodegradation of fluorobenzene (FB) under a number of operating conditions, which included variation in the concentration of FB in the feed stream (up to 180 mg l−1) and temporary suspension of feeding. Degradation of FB was followed for a period of 8 months under a continuous flow regime. During the operation of the UFBR, FB was never detected in the reactor effluent, being biodegraded by the microbial biofilm or adsorbed to the granular activated carbon (GAC). Biodegradation of FB was observed from the beginning of the reactor operation, and overall, it accounted for 50% of the total amount fed to the bioreactor. High organic loads of FB (210–260 mg d−1 dm−3) were found to affect the biological removal efficiency, possibly due to an inhibitory effect caused by the higher FB concentrations fed to the bioreactor (149–179 mg l−1). When FB feeding was suspended for 1 month, biodegradation continued, indicating that the adsorbed FB became bioavailable. Biofilm bacterial dynamics were followed throughout the UFBR operation by denaturing gradient gel electrophoresis and plate-counting techniques, showing that a quite stable community was found in the bioreactor, and this was mainly attributed to the high selective pressure exerted by the presence of FB.


Granular Activate Carbon Fluorobenzene Microbial Dynamic Substrate Feeding Bioreactor Operation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



M.F. Carvalho, R. Ferreira Jorge, and I.S. Henriques acknowledge research grants from Fundação para a Ciência e Tecnologia (FCT), Portugal (BD/21839/99, BPD/6977/2001, and SFRH/BD/5275/2001, respectively) and Fundo Social Europeu (III Quadro Comunitário de Apoio). This work was supported in part by the European Community's Human Potential Programme under contract HPRTN-CT-2002-00213 [BIOSAP]. The authors thank Pedro Moradas-Ferreira (IBMC, Porto) for his support.


  1. Abu-Salah K, Shelef G, Levanon D, Armon R, Dosoretz CG (1996) Microbial degradation of aromatic and polyaromatic toxic compounds adsorbed on powdered activated carbon. J Biotechnol 51:265–272CrossRefGoogle Scholar
  2. Armenante PM (1998) Suspended-biomass and fixed-film reactors. In: Lewandowski GA, DeFilippi LJ (eds) Biological treatment of hazardous wastes. Wiley, New York, pp 1–34Google Scholar
  3. Banks RE, Smart BE, Tatlow JC (1994) Organofluorine chemistry: principles and commercial applications. Plenum, New YorkGoogle Scholar
  4. Caldeira M, Heald SC, Carvalho MF, Bull AT, Vasconcelos I, Castro PML (1999) 4-Chlorophenol degradation by a bacterial consortium: development of a granular activated carbon biofilm reactor. Appl Microbiol Biotechnol 52:722–729CrossRefPubMedGoogle Scholar
  5. Carvalho MF, Vasconcelos I, Bull AT, Castro PML (2001) A GAC biofilm reactor for the continuous degradation of 4-chlorophenol: treatment efficiency and microbial analysis. Appl Microbiol Biotechnol 57:419–426CrossRefPubMedGoogle Scholar
  6. Carvalho MF, Alves CCT, Ferreira MIM, De Marco P, Castro PML (2002) Isolation and initial characterization of a bacterial consortium able to mineralize fluorobenzene. Appl Environ Microbiol 68:102–105CrossRefPubMedGoogle Scholar
  7. Carvalho MF, Ferreira Jorge R, Pacheco CC, De Marco P, Castro PML (2005) Isolation and properties of a pure bacterial strain capable of fluorobenzene degradation as sole carbon and energy source. Environ Microbiol 7:294–298CrossRefPubMedGoogle Scholar
  8. Casserly C, Erijman L (2003) Molecular monitoring of microbial diversity in an UASB reactor. Biodeterior Biodegrade 52:7–12CrossRefGoogle Scholar
  9. DeFilippi LJ, Lupton S (1998) Introduction to microbiological degradation of aqueous waste and its application using a fixed-film reactor. In: Lewandowski GA, DeFilippi LJ (eds) Biological treatment of hazardous wastes. Wiley, New York, pp 1–34Google Scholar
  10. El Frantoussi S, Belkacemi M, Top EM, Mahillon J, Naveau H, Agathos SN (1999) Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies. Environ Sci Technol 33:2992–3001CrossRefGoogle Scholar
  11. Feakin SJ, Blackburn E, Burns RG (1995) Inoculation of granular activated carbon in a fixed bed with s-triazine-degrading bacteria as a water treatment process. Water Res 29:819–825CrossRefGoogle Scholar
  12. Freitas dos Santos LM, Lamarca D, Gilges M, New A (1999) Biodegradation of chlorobenzene, iodobenzene and fluorobenzene: batch and continuous experiments. Trans IchemE 77:43–48Google Scholar
  13. Heipieper HJ, Keweloh H, Rehm HJ (1991) Influence of phenols on growth and membrane permeability of free and immobilized Escherichia coli. Appl Environ Microbiol 57:1213–1217PubMedGoogle Scholar
  14. Hekmat D, Linn A, Stephan M, Vortmeyer D (1997) Biodegradation dynamics of aromatic compounds from waste air in a trickle-bed reactor. Appl Microbiol Biotechnol 48:129–134CrossRefGoogle Scholar
  15. Henriques IS, Almeida A, Cunha A, Correia A (2004) Molecular sequence analysis of prokaryotic diversity in the middle and outer sections of the Portuguese estuary Ria de Aveiro. FEMS Microbiol Ecol 49:269–279CrossRefGoogle Scholar
  16. Ivancev-Tumbas I, Dalmacija B, Tamas Z, Karlovic E (1998) Reuse of biologically regenerated activated carbon for phenol removal. Water Res 32:1085–1094CrossRefGoogle Scholar
  17. Jonge RJ, Breure AM, van Andel JG (1996) Bioregeneration of powdered activated carbon (PAC) loaded with aromatic compounds. Water Res 30:875–882CrossRefGoogle Scholar
  18. Key BD, Howell RD, Criddle CS (1997) Fluorinated organics in the biosphere. Environ Sci Technol 31:2445–2454CrossRefGoogle Scholar
  19. Khodadoust AP, Wagner JA, Suidan MT, Brenner RC (1997) Anaerobic treatment of PCP in fluidized-bed GAC bioreactors. Water Res 31:1776–1786CrossRefGoogle Scholar
  20. Klecka GM, McDaniel SG, Wilson PS, Carpenter CL, Clarck JE, Thomas A, Spain JC (1996) Field evaluation of a granular activated carbon fluid-bed bioreactor for treatment of chlorobenzene in groundwater. Environ Prog 15:93–107CrossRefGoogle Scholar
  21. LaPara TM, Nakatsu CH, Pantea LM, Alleman JE (2002) Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE. Water Res 36:638–646CrossRefPubMedGoogle Scholar
  22. Lee CM, Lu CJ, Chuang MS (1994) Effects of immobilized cells on the biodegradation of chlorinated phenols. Water Sci Technol 30:87–90Google Scholar
  23. Luxmy BS, Nakajima F, Yamamoto K (2000) Analysis of bacterial community in membrane-separation bioreactors by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). Water Sci Technol 41:259–268Google Scholar
  24. Massol-Deyá A, Weller R, Ríos-Hernández L, Zhou J-Z, Hichey RF, Tiedje JM (1997) Succession and convergence of biofilm communities in fixed-film reactors treating aromatic hydrocarbons in groundwater. Appl Environ Microbiol 63:270–276PubMedGoogle Scholar
  25. Moteleb MA, Suidan MT, Kim J, Maloney SW (2002) Pertubated loading of a formaldehyde waste in an anaerobic granular activated fluidized bed reactor. Water Res 36:3775–3785CrossRefPubMedGoogle Scholar
  26. Nandy T, Kaul SN (2001) Anaerobic pre-treatment of herbal-based pharmaceutical wastewater using fixed-film reactor with recourse to energy recovery. Water Res 35:351–362CrossRefPubMedGoogle Scholar
  27. Schäfer H, Muyzer G (2001) Denaturing gradient gel electrophoresis in marine microbial ecology. Methods Microbiol 30:425–468CrossRefGoogle Scholar
  28. Schlötelburg C, von Wintzingerode C, Hauck R, von Wintzingerode F, Hegemann W, Göbel UB (2002) Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropane. FEMS Microbiol Ecol 39:229–237CrossRefGoogle Scholar
  29. Shi J, Zhao XD, Hickey RF, Voice TC (1995) Role of adsorption in granular activated carbon-fluidized bed reactors. Water Environ Res 67:302–309CrossRefGoogle Scholar
  30. Smith NR, Yu Z, Mohn WW (2003) Stability of the bacterial community in a pulp mill effluent treatment system during normal operation and a system shutdown. Water Res 37:4873–4884CrossRefPubMedGoogle Scholar
  31. Song B, Palleroni NJ, Haggblom MM (2000) Isolation and characterization of diverse halobenzoate-degrading denitrifying bacteria from soils and sediments. Appl Environ Microbiol 66:3446–3453CrossRefPubMedGoogle Scholar
  32. Speitel GE, Lu CJ, Turakhia M, Zhu XJ (1989) Biodegradation of trace concentrations of substituted phenols in granular activated carbon columns. Environ Sci Technol 23:66–74CrossRefGoogle Scholar
  33. Sun AK, Wood TK (1997) Trichloroethylene mineralization in a fixed-film bioreactor using a pure culture expressing constitutively toluene ortho-monooxygenase. Biotechnol Bioeng 55:674–685CrossRefGoogle Scholar
  34. Tresse O, Lorrain M-J, Rho D (2002) Population dynamics of free-floating and attached bacteria in a styrene-degrading biotrickling filter analyzed by denaturing gradient gel electrophoresis. Appl Microbiol Biotechnol 59:585–590CrossRefPubMedGoogle Scholar
  35. Vargas C, Song B, Camps M, Haggblom MM (2000) Anaerobic degradation of fluorinated aromatic compounds. Appl Microbiol Biotechnol 53:342–347CrossRefPubMedGoogle Scholar
  36. Wunderwald U, Hofrichter M, Kreisel G, Fritsche W (1998) Transformation of difluorinated phenols by Penicillium frequentans Bi 7/2. Biodegradation 8:379–3851CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. F. Carvalho
    • 1
  • R. Ferreira Jorge
    • 1
  • C. C. Pacheco
    • 2
  • P. De Marco
    • 2
  • I. S. Henriques
    • 3
  • A. Correia
    • 3
  • P. M. L. Castro
    • 1
  1. 1.Escola Superior de BiotecnologiaUniversidade Católica PortuguesaPortoPortugal
  2. 2.IBMCUniversidade do PortoPortoPortugal
  3. 3.Departamento de Biologia, Universidade de AveiroCampus Universitário de SantiagoAveiroPortugal

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