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Everything Is Everywhere: Physiological Responses of the Mediterranean Sea and Eastern Pacific Ocean Epiphyte Cobetia Sp. to Varying Nutrient Concentration

  • Environmental Microbiology
  • Published:
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Abstract

Bacteria are essential in the maintenance and sustainment of marine environments (e.g., benthic systems), playing a key role in marine food webs and nutrient cycling. These microorganisms can live associated as epiphytic or endophytic populations with superior organisms with valuable ecological functions, e.g., seagrasses. Here, we isolated, identified, sequenced, and exposed two strains of the same species (i.e., identified as Cobetia sp.) from two different marine environments to different nutrient regimes using batch cultures: (1) Cobetia sp. UIB 001 from the endemic Mediterranean seagrass Posidonia oceanica and (2) Cobetia sp. 4B UA from the endemic Humboldt Current System (HCS) seagrass Heterozostera chilensis. From our physiological studies, both strains behaved as bacteria capable to cope with different nutrient and pH regimes, i.e., N, P, and Fe combined with different pH levels, both in long-term (12 days (d)) and short-term studies (4 d/96 h (h)). We showed that the isolated strains were sensitive to the N source (inorganic and organic) at low and high concentrations and low pH levels. Low availability of phosphorus (P) and Fe had a negative independent effect on growth, especially in the long-term studies. The strain UIB 001 showed a better adaptation to low nutrient concentrations, being a potential N2-fixer, reaching higher growth rates (μ) than the HCS strain. P-acquisition mechanisms were deeply investigated at the enzymatic (i.e., alkaline phosphatase activity, APA) and structural level (e.g., alkaline phosphatase D, PhoD). Finally, these results were complemented with the study of biochemical markers, i.e., reactive oxygen species (ROS). In short, we present how ecological niches (i.e., MS and HCS) might determine, select, and modify the genomic and phenotypic features of the same bacterial species (i.e., Cobetia spp.) found in different marine environments, pointing to a direct correlation between adaptability and oligotrophy of seawater.

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Data and Availability and Code Availability

This manuscript contains previously unpublished data. The name of the repository and accession number(s) are CP058244 to CP058245 (Cobetia sp. UIB 001) and CP059843 to CP059845 (Cobetia sp. 4B UA).

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Acknowledgements

VFJ acknowledges the travel funds provided by the Santander Iberoamérica 2019–2020 fellowship. We are grateful to Prof. Mario Edding and Felipe Sáez of the Laboratorio de Botánica Marina at the Universidad Católica del Norte for providing us with leaves of H. chilensis.

Funding

NSRA was funded through the Ministerio de Economía, Industria y Competitividad-Agencia Estatal de Investigación and the European Regional Development Funds project (CTM2016-75457-P). PE acknowledges the financial support of the research grants Fondecyt Iniciación 11170837, FONDEQUIP EQM120137, and REDI170403.

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Authors and Affiliations

  1. Marine Ecology and Systematics (MarES), Department of Biology, University of the Balearic Islands, Palma, Spain

    Víctor Fernández-Juárez & Nona S. R. Agawin

  2. Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

    Daniel Jaén-Luchoro

  3. Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden

    Daniel Jaén-Luchoro

  4. Instituto de Ciencias Naturales Alexander von Humboldt, Universidad de Antofagasta, Antofagasta, Chile

    Jocelyn Brito-Echeverría & Pedro Echeveste

  5. Grup de Microbiologia, Department of Biology, University of the Balearic Islands, Palma, Spain

    Antoni Bennasar-Figueras

  6. Instituto Milenio de Oceanografía, Concepción, Chile

    Pedro Echeveste

Authors
  1. Víctor Fernández-Juárez
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  2. Daniel Jaén-Luchoro
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  3. Jocelyn Brito-Echeverría
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  4. Nona S. R. Agawin
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  5. Antoni Bennasar-Figueras
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  6. Pedro Echeveste
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Contributions

VFJ and PE designed the experiments. VFJ conducted all the laboratory experiments and isolated the UIB 001 strain. JBE isolated the Pacific 4B UA strain. DJL conducted the bioinformatic analyses. All the authors, VFJ, DJL, JBE, ABF, NSRA, and PE, led the writing of the paper.

Corresponding author

Correspondence to Víctor Fernández-Juárez.

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The authors declare no competing interests.

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Fernández-Juárez, V., Jaén-Luchoro, D., Brito-Echeverría, J. et al. Everything Is Everywhere: Physiological Responses of the Mediterranean Sea and Eastern Pacific Ocean Epiphyte Cobetia Sp. to Varying Nutrient Concentration. Microb Ecol 83, 296–313 (2022). https://doi.org/10.1007/s00248-021-01766-z

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