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A Highly Active Alkaline Phosphatase from the Marine Bacterium Cobetia

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Abstract

An alkaline phosphatase with unusually high specific activity has been found to be produced by the marine bacterium Cobetia marina (strain KMM MC-296) isolated from coelomic liquid of the mussel Crenomytilus grayanus. The properties of enzyme, such as a very high specific activity (15000 DE U/1 mg of protein), no activation with divalent cations, resistance to high concentrations of inorganic phosphorus, as well as substrate specificity toward 5′ nucleotides suggest that the enzyme falls in an intermediate position between unspecific alkaline phosphatases (EC 3.1.3.1) and 5′ nucleotidases (EC 3.1.3.5).

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Acknowledgments

We are grateful to Mrs. Shepetova for helpful discussion and translation this article into English. This work was supported by grants from the Russian Foundation of Fundamental Research (02-04-49517), the Ministry of Industry, Science and Technologies of the Russian Federation (2-2-16), and the Russian Academy of Sciences Program of Fundamental Research Physico-chemical Biology (03-1-05-002).

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok-22, 690022, Russia

    E. Yu Plisova, L.A. Balabanova, E.P. Ivanova, V. B. Kozhemyako, V. V. Mikhailov, E.V. Agafonova & V.A. Rasskazov

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  1. E. Yu Plisova
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  2. L.A. Balabanova
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  3. E.P. Ivanova
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  4. V. B. Kozhemyako
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  5. V. V. Mikhailov
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  6. E.V. Agafonova
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  7. V.A. Rasskazov
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Correspondence to V.A. Rasskazov.

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Yu Plisova, E., Balabanova, L., Ivanova, E. et al. A Highly Active Alkaline Phosphatase from the Marine Bacterium Cobetia . Mar Biotechnol 7, 173–178 (2005). https://doi.org/10.1007/s10126-004-3022-4

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  • DOI: https://doi.org/10.1007/s10126-004-3022-4

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