Abstract
The Lascaux Cave contains a remarkable set of paintings from the Upper Palaeolithic. Shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a Fusarium solani species complex. Benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. In this study, we carried out a sampling in most of the cave halls and galleries. Sequence analysis and isolation methods detected that the most abundant genera of bacteria were Ralstonia and Pseudomonas. We suggest that, as a result of years of benzalkonium chloride treatments, the indigenous microbial community has been replaced by microbial populations selected by biocide application.
References
Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169
Bastian F, Alabouvette C (2009) Lights and shadows on the conservation of a rock art cave: the case of Lascaux Cave. Int J Speleol 38:55–60
Bastian F, Alabouvette C, Saiz-Jimenez C (2009a) Bacteria and free-living amoeba in the Lascaux Cave. Res Microbiol 160:38–40
Bastian F, Alabouvette C, Saiz-Jimenez C (2009b) The impact of arthropods on fungal community structure in Lascaux Cave. J Appl Microbiol 106:1456–1462
Bastian F, Bouziri L, Nicolardot B, Ranjard L (2009b) Impact of wheat straw decomposition on successional patterns of soil microbial community structure. Soil Biol Biochem 41:262–275
Brunet J, Vidal P, Vouvé J (1987) The conservation of rock art. Studies and documents on the cultural heritage No. 7, UNESCO (233 p.)
Chelius MK, Moore JC (2004) Molecular phylogenetic analysis of Archaea and Bacteria in Wind Cave, South Dakota. Geomicrobiol J 21:123–134
Chelius MK, Beresford G, Horton H, Quirk M, Selby G, Simpson RT, Horrocks R, Moore JC (2009) Impacts of alterations of organic inputs on the bacterial community within the sediments of Wind Cave, South Dakota, USA. Int J Speleol 38:1–10
Chen W-M, Laevens S, Lee T-M, Coenye T, De Vos P, Mergeay M, Vandamme P (2001) Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735
Dupont J, Jacquet C, Dennetière B, Lacoste S, Bousta F, Orial G, Cruaud C, Couloux A, Roquebert MF (2007) Invasion of the French Paleolithic painted cave of Lascaux by members of the Fusarium solani species complex. Mycologia 99:526–533
Gonzalez JM, Portillo MC, Saiz-Jimenez C (2006) Metabolically active Crenarchaeota in Altamira Cave. Naturwissenschaften 93:42–45
Holmes AJ, Tujula NA, Holley M, Contos A, James JM, Rogers P, Gillings MR (2001) Phylogenetic structure of unusual aquatic microbial formations in Nullarbor Caves, Australia. Environ Microbiol 3:256–264
Ikner LA, Toomey R, Nolan G, Neilson J, Pryor B, Maier R (2007) Culturable microbial diversity and the impact of tourism in Kartchner Caverns, Arizona. Microb Ecol 53:30–40
Jurado V, Groth I, Gonzalez JM, Laiz L, Saiz-Jimenez C (2005) Agromyces subbeticus sp. nov., isolated from a cave in southern Spain. Int J Syst Evol Microbiol 55:1897–1901
Jurado V, Gonzalez JM, Laiz L, Saiz-Jimenez C (2006) Aurantimonas altamirensis sp. nov., a member of the order Rhizobiales isolated from Altamira Cave. Int J Syst Evol Microbiol 56:2583–2585
Jurado V, Boiron P, Kroppenstedt RM, Laurent F, Couble A, Laiz L, Klenk HP, Gonzalez JM, Saiz-Jimenez C, Mouniee D, Bergeron E, Rodriguez-Nava V (2008) Nocardia altamirensis sp. nov., isolated from Altamira Cave, Cantabria, Spain. Int J Syst Evol Microbiol 58:2210–2214
Kisand V, Wikner J (2003) Combining culture-dependent and -independent methodologies for estimation of richness of estuarine bacterioplankton consuming riverine dissolved organic matter. Appl Environ Microbiol 69:3607–3616
Lacanette D, Malaurent P, Caltagirone JP, Brunet J (2007) Etude des transferts de masse leur dans la grotte de Lascaux: le suivi climatique et le simulateur. Karstologia 50:19–30
Laiz L, Miller AZ, Jurado V, Akatova E, Sanchez-Moral S, Gonzalez JM, Dionísio A, Macedo MF, Saiz-Jimenez C (2009) Isolation of five Rubrobacter strains from biodeteriorated monuments. Naturwissenschaften 96:71–79
Langsrud S, Sundheim G, Borgmann-Strahsen R (2003) Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp. J Appl Microbiol 95:874–882
Lefèvre M (1974) La “maladie verte” de Lascaux. Stud Conserv 19:126–156
Macalady JL, Jones DS, Lyon EH (2007) Extremely acidic, pendulous cave wall biofilms from the Frasassi cave system, Italy. Environ Microbiol 9:1402–1414
Nagai K, Ohta S, Zenda H, Matsumoto H, Makino M (1996) Biochemical characterization of a Pseudomonas fluorescens strain isolated from a benzalkonium chloride solution. Biol Pharm Bull 19:873–875
Northup DE, Lavoie KH (2003) Diverse microbial communities inhabiting ferromanganese deposits in Lechuguilla and Spider Caves. Environ Microbiol 5:1071–1086
Orial G, Mertz JD (2006) Etude et suivi des phénomènes microbiologiques. Monumental 2:76–86
Patrauchan MA, Oriel PJ (2003) Degradation of benzyldimethylalkylammonium chloride by Aeromonas hydrophila sp. K. J Appl Microbiol 94:266–272
Piñar G, Saiz-Jimenez C, Schabereiter-Gurtner C, Blanco-Varela MT, Lubitz W, Rölleke S (2001) Archaeal communities in two disparate deteriorated ancient wall paintings: detection, identification and temporal monitoring by DGGE. FEMS Microbiol Ecol 37:45–54
Saiz-Jimenez C (1999) Biogeochemistry of weathering processes in monuments. Geomicrobiol J 16:27–37
Saiz-Jimenez C, Hermosin B (1999) Thermally assisted hydrolysis and methylation of dissolved organic matter in dripping waters from the Altamira Cave. J Anal Appl Pyrol 49:337–347
Schabereiter-Gurtner C, Saiz-Jimenez C, Piñar G, Lubitz W, Rolleke S (2002a) Altamira cave Paleolithic paintings harbor partly unknown bacterial communities. FEMS Microbiol Lett 211:7–11
Schabereiter-Gurtner C, Saiz-Jimenez C, Piñar G, Lubitz W, Rolleke S (2002b) Phylogenetic 16 S rRNA analysis reveals the presence of complex and partly unknown bacterial communities in Tito Bustillo Cave, Spain, and on its Paleolithic paintings. Environ Microbiol 4:392–400
Schabereiter-Gurtner C, Saiz-Jimenez C, Piñar G, Lubitz W, Rolleke S (2004) Phylogenetic diversity of bacteria associated with Paleolithic paintings and surrounding rock walls in two Spanish caves (Llonin and La Garma). FEMS Microbiol Ecol 47:235–247
Sauer K, Rickard AH, Davies DG (2007) Biofilms and biocomplexity. Microbe 2:347–353
Sire MA (2006) De l’élimination des champignons au constat d’état. Monumental 2:68–75
Zhou JP, Gu YQ, Zou CS, Mo MH (2007) Phylogenetic diversity of bacteria in an earth-cave in Guizhou Province, Southwest of China. J Microbiol 45:105–112
Acknowledgements
The first author and the research project “Microbiology-Microclimate” were supported by the Ministry of Culture and Communication, France. Authors acknowledge support from the Ministry of Culture and Communication and from project CGL2006-07424/BOS, Spain. This is a TCP CSD2007-00058 paper. The collaboration of the Lascaux restoration team is highly appreciated. We thank Marisa Chelius for valuable comments on the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bastian, F., Alabouvette, C., Jurado, V. et al. Impact of biocide treatments on the bacterial communities of the Lascaux Cave. Naturwissenschaften 96, 863–868 (2009). https://doi.org/10.1007/s00114-009-0540-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00114-009-0540-y