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Implementation of rapid multiplication and tissue culture methods in Third World countries

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Conclusions

In general, almost all programs have experienced difficulties of virus infections and/or other diseases in the cutting operations. A careful and systematic testing program must be developed with micro-propagation methods. More production ofin vitro plantlets and cuttings is usually needed in the early years of a program than is needed later. The re-use of non-certified seed derived from high quality certified seed eventually reduces the demand for certified seed. Over production of cuttings is not an uncommon occurrence as a seed program matures.

High quality seed does not always result in higher yields of ware potatoes. An economic analysis of the system should be initiated early in the development of a program. Studies on seed marketing and demand are usually beneficial to the proper planning and executing of a seed production program.

In vitro propagation and rapid multiplication of pathogen-tested cultivars are fast becoming an important factor in developing self-sufficiency in seed potato production in Third World countries. The reduced number of field generations needed to supply sufficient quantities of quality seed is becoming a reality in countries where climatic and economic constraints are limiting.

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Literature Cited

  1. Bercks, R. 1967. Methodische Untersuchungen über den serologischen Nachweis pflanzenpathogener Viren mit dem Bentonit-Flockungstest, dem Latextest und dem Barimsulfat-test. Phytopathol Z 58:1–17.

    Google Scholar 

  2. Bryan, J.E., M. Jackson and N. Melendez G. 1981. Rapid multiplication techniques for potato. CIP Bul. 20 pp. International Potato Center, Lima, Peru.

    Google Scholar 

  3. Clark, M.F. and A.N. Adams. 1977. Characteristics of the microplate method of enzyme linked inmunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483.

    Article  PubMed  CAS  Google Scholar 

  4. Cole, E.F. and N.S. Wright, 1967. Propagation of potato by stem cuttings. Am Potato J 44:301–304.

    Google Scholar 

  5. Ezeta, F.N. and U.C. Scheidegger. 1985. Basic seed: a new production and distribution program for Peru.In: CIP Circular 13(2): 1–4. International Potato Center, Lima, Peru.

    Google Scholar 

  6. Franco, J. 1974. Ensayos preliminarios de enraizamiento de hojas y foliolos de papa. Invest Agropecu (Peru) 4(1–2):56–61.

    Google Scholar 

  7. Fribourg, C.E. and J. Nakashima. 1984. An improved latex agglutination test for routine detection of potato viruses. Potato Res 27:237–249.

    Article  Google Scholar 

  8. Goodwin, P.B. 1981. Rapid propagation of potato by single node cuttings. Field Crops Res 4:165–173.

    Article  Google Scholar 

  9. Hamann, U. 1974. Intensivvermehrung der Kartoffel in der l. Stufe der Erhaltungszucht.In: The Potato. Institut Ziemniaka Bonin k. Koszalina. (Poland). pp 107–126.

    Google Scholar 

  10. Horton, D.E. and H. Fano. 1985. Potato Atlas. 135 pp. International Potato Center, Lima, Peru.

    Google Scholar 

  11. Horton, D.E., Y.C. Kim, B.H. Hahn, K.K. Kim, I.G. Mok and B.N. Lee. 1987. Korea’s seed potato program: organization, impact and issues. International Potato Center, Lima Peru. pp 68.

    Google Scholar 

  12. Kim, Y.C., K.K. Kim and B.H. Hahn. 1986. Impact of the South Korean seed potato program. In CIP Circular 14(4): 1–5. International Potato Center, Lima, Peru.

    Google Scholar 

  13. Lauer, F.I. 1977. Tubers from leaf bud cuttings: a tool for potato seed certification and breeding programs. Am Potato J 54:457–464.

    Google Scholar 

  14. Mellor, F.C. and R. Stace-Smith. 1967. Eradication of potato virus X by thermotherapy. Phytopathology 57:674–678.

    PubMed  CAS  Google Scholar 

  15. Monares, A., 1981. The potato seed system in the Andean Region: The cases of Peru, Equador and Colombia. PhD thesis. Cornell University. Unpublished.

  16. Ortega, E., A. Vargas, F. Paz and R. Wissar. 1987. Producción de semillas de papa en Venezuela. In Proceedings XIII Reunion de Asociacion Latinoamericana de la Papa, pp 137–141.

  17. Potts, M.J. 1986. The Burundi potato program: a single integrated approach to crop management. In CIP Circular 14(1): 1–6. International Potato Center, Lima, Peru.

    Google Scholar 

  18. Roca, W.M., J.E. Bryan and M.R. Roca. 1979. Tissue culture for the international transfer of potato genetic resources. Am Potato J 56:1–10.

    Article  Google Scholar 

  19. Roca, W.M., N.O. Espinoza, M.R. Roca and J.E. Bryan. 1978. A tissue culture method for the rapid propagation of potatoes. Am Potato J 55:691–701.

    Google Scholar 

  20. Salazar, L. 1979. Aplicación de la técnica serológica de los conjugados enzimáticos (ELIZA) para diagnosticar virus de la papa. Fitopathología 14:1–9.

    Google Scholar 

  21. Van Uyen, N. and P. van der Zaag. 1983. Vietnamese farmers use tissue culture for commercial potato production. Am Potato J 60:873–879.

    Google Scholar 

  22. Vittorelli, C. and F.N. Ezeta. 1987. Modelo integrado para la producción de semilla básica de papa en el Perú: advances y proyecciones. In Proceedings XIII Reunión de Asociación Latinoamericana de la Papa. pp 102–114.

  23. Wang, P.J. and C.Y. Hu. 1982. In vitro mass tuberization and virus-free seed potato production in Taiwan. Am Potato J 59:33–37.

    Google Scholar 

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Bryan, J.E. Implementation of rapid multiplication and tissue culture methods in Third World countries. American Potato Journal 65, 199–207 (1988). https://doi.org/10.1007/BF02854452

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