Skip to main content
SpringerLink
Log in

A liquid chromatographic lon-pairing method for simultaneous determination of benazepril hydrochloride, fosinopril sodium, ramipril and hydrochlorothiazide in pharmaceutical formulations

  • Originals
  • Column Liquid Chromatography
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A rapid and accurate reversed-phase ion-pairing liquid chromatographic method with UV detection was developed for the simultaneous assay of the angiotensin-converting enzyme inhibitors benazepril hydrochloride, fosinopril sodium and ramipril, and the diuretic hydrochorothiazide.

The separation was achieved on a LC-8 (125×4.0 mml.D.; 5μm particle size) column.

The mobile phase consisted of 20 mM sodium heptanesulphonate (pH=2.5) and methanol (32:68v/v).

Validation of the method showed it to be precise, accurate and linear over the concentration range of analysis with a limit of detection of 1 ng for hydrochorothiazide, 2 ng for ramipril and benazepril and 8 ng for fosinopril.

The method developed was applied to the analysis of three different binary commercial formulations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bonazzi, D.; Gotti, R.; Andrisano, V.; Cavrini, V.J. Pharm. Biomed. Anal. 1997,16, 431–438.

    Article  CAS  Google Scholar 

  2. Kaiser, G.; Ackermann, R.; Dieterle, W.; Dubois, J.P.J. Chromatogr. B 1987,419, 123–133.

    CAS  Google Scholar 

  3. Sioufi, A.; Pommier, F.; Kaiser, G.; Dubois, J.P.J. Chromatogr. B 1988,434, 239–246.

    CAS  Google Scholar 

  4. Panderi, I.E.J. Pharm. Biomed. Anal. 1999,21, 257–265.

    Article  CAS  Google Scholar 

  5. Kirschbaum, J.; Noroski, J.; Cosey, A.; Mayo, D.; Adamoics, J.J. Chromatogr. 1990,507, 165–170.

    Article  CAS  Google Scholar 

  6. Swanson, B.N.; Stauber, K.L.; Alpaugh, W.C.; Weinstein, S.H.Anal. Biochem. 1985,148, 401–407.

    Article  CAS  Google Scholar 

  7. Lozano, R.; Warren, F.V.; Perlman, S.; Joseph, J.M.J. Pharm. Biomed. Anal. 1995,13, 139–148.

    Article  CAS  Google Scholar 

  8. Magri', A.L.; Balestrieri, F.; Magri', A.D.; Marini, D.Talanta 1995,42, 1719–1723.

    Article  Google Scholar 

  9. Aboul-Enein, H.Y.; Thiffault, C.Anal. Lett. 1991,24, 2217–2224.

    CAS  Google Scholar 

  10. Abdellatef, H.E.; Ayad, M.M.; Taha, E.A.J. Pharm. Biomed. Anal. 1999,18, 1021–1027.

    Article  CAS  Google Scholar 

  11. Eckert, H.G.; Munscher, G.; Oekonomopulos, R.; Strecker, H.; Urbach, H.; Wissmann, H.Arzneim.-Forsch./Drug Res. 1985 35 (11), 1251–1256.

    CAS  Google Scholar 

  12. Farthing, D.; Fakhry, I.; Ripley, E.B.D.; Sica, D.J. Pharm. Biomed. Anal. 1998,17, 1455–1459.

    Article  CAS  Google Scholar 

  13. Gumieniczek, A.; Hopkala, H.Pharm. Acta Helv. 1998,73, 183–185.

    Article  CAS  Google Scholar 

  14. Gustafsson, S.; Eriksson, I.; Nilsson, B.M.J. Chromatogr. 1990,506, 75–83.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanita, Viale Regina Elena, 299, 00161, Rome, Italy

    L. Manna, L. Valvo & S. Alimonti

Authors
  1. L. Manna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Valvo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Alimonti
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Manna, L., Valvo, L. & Alimonti, S. A liquid chromatographic lon-pairing method for simultaneous determination of benazepril hydrochloride, fosinopril sodium, ramipril and hydrochlorothiazide in pharmaceutical formulations. Chromatographia 53 (Suppl 1), S271–S275 (2001). https://doi.org/10.1007/BF02490341

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02490341

Key Words

Search

Navigation