Calcified Tissue International

, Volume 34, Issue 1, pp 285–290

Bone acid phosphatase: Tartrate-resistant acid phosphatase as a marker of osteoclast function

  • Cedric Minkin
Laboratory Investigations

Summary

Organ cultures of newborn mouse calvaria were used to test the hypothesis that tartrate-resistant acid phosphatase might serve as a biochemical marker for osteoclast function. When bone resorption was stimulatedin vitro with either parathyroid hormone or 1,25(OH)2D3, there was a significant increase in both tartrate-resistant and tartrate-sensitive acid phosphatase activity in the medium relative to cultured controls. Tartrate-resistant activity was localized histochemically primarily over the osteoclast and appeared as three distinct activity bands when electrophoresed on polyacrylamide gels. The tartrate-sensitive activity was found primarily associated with bone cells other than the osteoclast using histochemical techniques, and was resolved into five bands on polyacrylamide gels. The results obtained from biochemical assays, histochemical observations, and polyacrylamide gel electrophoresis suggest that bone resorptionin vitro results in the release of tartrate-resistant acid phosphatase from osteoclasts and tartrate-sensitive acid phosphatase from other bone cells as well as osteoclasts. Tartrate-resistant acid phosphatases of bone may be suitable biochemical probes for osteoclast function, but it will be necessary to achieve further purification in order to develop analytical methods with sufficient sensitivity and specificity (e.g., immunochemical) to ensure precise localization and quantitation.

Key words

Acid phosphatase, tartrate-resistant Isoenzymes Bone resorption Osteoclast 

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References

  1. 1.
    Horton, J., Oppenheim, J. J., Mergenhagen, S. E.: A role for cell-mediated immunity in the pathogenesis of periodontal disease, J. Periodontol.45:351–360, 1974PubMedGoogle Scholar
  2. 2.
    Walker, D. G.: Osteopetrosis in mice cured by temporary parabiosis, Science180:875–876, 1973PubMedGoogle Scholar
  3. 3.
    Hall, B. K.: The origin and fate of osteoclasts, Anat. Rec.183:1–12, 1975CrossRefPubMedGoogle Scholar
  4. 4.
    Kahn, A. J., Stewart, C. C., Teitelbaum, S. L.: Contactmediated bone resorption by human monocytesin vitro, Science199:988–990, 1978PubMedGoogle Scholar
  5. 5.
    Hammarstrom, L. E., Hanker, J. S., Toverud, S. U.: Cellular differences in acid phosphatase isoenzymes in bone and teeth, Clin. Orthop.78:151–167, 1971PubMedGoogle Scholar
  6. 6.
    Wergedal, J. E.: Characterization of bone acid phosphatase activity, Proc. Soc. Exp. Biol. Med.134:244–247, 1970PubMedGoogle Scholar
  7. 7.
    Anderson, T. R., Toverud, S. U.: Purification and partial characterization of two acid phosphatases from rat bone, Calcif. Tissue Int.27:219–226, 1979PubMedGoogle Scholar
  8. 8.
    Minkin, C., Posek, R., Newbrey, J.: Mononuclear phagocytes and bone resorption: identification and preliminary characterization of a bone-derived chemotactic factor, Metab. Bone Dis. Rel. Res. (in press)Google Scholar
  9. 9.
    Sokal, R. R., Rohlf, F. J.: Biometry. The Principles and Practice of Statistics in Biological Research. W. H. Freeman, San Francisco, 1969Google Scholar
  10. 10.
    Li, C. Y., Yam, L. T., Lam, K. W.: Acid phosphatase isoenzyme in human leukocytes in normal and pathologic conditions. J. Histochem. Cytochem.18:473–481, 1970PubMedGoogle Scholar
  11. 11.
    Susi, F. R., Goldhaber, P., Jennings, J. M.: Histochemical and biochemical study of acid phosphatase in resorbing bone in culture, Am. J. Physiol.211:959–962, 1966PubMedGoogle Scholar
  12. 12.
    Vaes, G.: On the mechanisms of bone resorption. The action of parathyroid hormone on the excretion and synthesis of lysosomal enzymes and on the extracellular release of acid by bone cells, J. Cell Biol.39:676–697, 1968CrossRefPubMedGoogle Scholar
  13. 13.
    Messer, H. H., Armstrong, W. D., Singer, L.: Influence of calcitonin on bone phosphatases and phosphate release in organ culture, Proc. Soc. Exp. Biol. Med.143:690–692, 1973PubMedGoogle Scholar
  14. 14.
    Lin, C., Fishman, W. H.: Microsomal and lysosomal acid phosphatase isoenzymes of mouse kidney. Characterization and separation, J. Histochem. Cytochem.20:487–498, 1972PubMedGoogle Scholar
  15. 15.
    Helminen, H. J., Ericsson, J. L. E., Rytoluoto, R., Vanha-Perttula, T.: Acid phosphatases of the rat ventral prostate. In M. Goland (ed.): Normal and Abnormal Growth of the Prostate, pp. 275–316. Charles C. Thomas, Springfield, Ill., 1975Google Scholar
  16. 16.
    Li, C. Y., Chuda, R. A., Lam, W. K. W.: Acid phosphatases in human plasma, J. Lab. Clin. Med.82:446–460, 1973PubMedGoogle Scholar
  17. 17.
    Yam, L. T.: Clinical significance of the human acid phosphatases. A review, Am. J. Med.56:604–616, 1974CrossRefPubMedGoogle Scholar
  18. 18.
    Holtrop, M. E., Raisz, L. G., King, G. J.: The response of osteoclasts to prostaglandin and osteoclast activating factor as measured by ultrastructural morphometry. Proceedings, Mechanisms of Localized Bone Loss, Calcif. Tissue Abstr. [Suppl.], pp. 13–20, 1978Google Scholar
  19. 19.
    Lieberherr, M., Vreven, J., Vaes, G.: The acid and alkaline phosphatases, inorganic pyrophosphatases and phosphoprotein phosphatases of bone. I. Characterization and assay, Biochim. Biophys. Acta293:160–169, 1973PubMedGoogle Scholar
  20. 20.
    Minkin, C., Hixon, H.: Bone acid phosphatase: isolation and characterization, J. Dent. Res.59A:502, 1980Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Cedric Minkin
    • 1
  1. 1.Department of Basic SciencesUniversity of Southern California School of DentistryLos AngelesUSA

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