Abstract
Cultured shoots ofRosa ‘Improved Blaze’ were used to determine the effects of sucrose and inorganic nitrogen on adventitious root formation. Shoots grown in media containing high sucrose concentrations (146.07–262.93 mM) produced more and longer roots than those grown in media containing 0–87.64 mM sucrose. This response to sucrose was related to the metabolism of sucrose rather than its osmotic properties since the use of mannitol and 3-O-methyl-α-D-glucopyranoside as osmotic substitutes did not reproduce the effect on rooting. The number and length of roots increased when the shoots were grown in media with the nitrogen concentration of the Murashige-Skoog (MS) salt formulation reduced from 60 to 7.5 mM. Neither nitrate (NO −3 ) nor ammonium (NH +4 ) alone at any of the concentrations tested had the effect on rooting that both had together in the ratio of the MS salt formulation. When the sucrose and nitrogen concentrations were both varied, the greatest rate of root initiation occurred on shoots grown in media with a high sucrose to nitrogen concentration ratio.
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References
Arrington LB, Shive JW (1963) Oxygen and carbon dioxide content of culture solutions in relation to cation and anion nitrogen absorption by tomato plants. Soil Sci 43:341–356
Bayley JM, King J, Gamborg OL (1972) The effect of the source of inorganic nitrogen on growth and enzymes of nitrogen assimilation in soybean and wheat cells in supension cultures. Planta 105:15–24
Clark HE, Shive JW (1934) The influence of pH of a culture solution on the rates of absorption of ammonium and nitrate nitrogen by the tomato plant. Soil Sci 37:203–225
Clark HE, Shive JW (1934) The influence of pH of a culture solution on the assimilation of ammonium and nitrate nitrogen by the tomato plant. Soil Sci 37:459–476
Davidson OW, Shive JW (1934) The influence of hydrogen-ion concentration of the culture solution upon the absorption and assimilation of nitrate and ammonium nitrogen by peach trees grown in sand cultures. Soil Sci 37:357–385
Dougall DK, Verma DC (1978) Growth and embryo formation in wild-carrot suspension cultures with ammonium as a sole nitrogen source. In Vitro 14:180–182
Fukanaga Y, King J, Child JJ (1978) The differential effects of TCA-cycle acids on the growth of plant cells cultured in liquid media containing various nitrogen sources. Planta 139:199–202
Gamborg OL, Shyluk JP (1970) The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol 45:598–600
Greenwood MS, Berlyn GP (1973) Sucrose-indole-3-acetic acid interactions on root regeneration byPinus lambertiana embryo cuttings. Am J Bot 60:42–47
Haissig BE (1974) Metabolism during adventitious root initiation and development. N Z J For Sci 4:324–337
Hasegawa PM (1979) In vitro propagation of rose. HortScience 14:610–612
Hasegawa PM (1980) Factors affecting shoot and root initiation from cultured rose shoot tips. J Am Soc Hortic Sci 105:216–220
Howard BH, Sykes JT (1966) Regeneration of the hop plant (Humulus lupulus L.) from softwood cuttings. II. Modification of the carbohydrate resources within the cutting. J Hortic Sci 41:155–163
Hyndman SE, Hasegawa PM, Bressan RA (1982) Stimulation of root initiation from cultured rose shoots through the use of reduced concentrations of mineral salts. HortScience 17:82–83
Kirkby EA, Mengel K (1967) Ionic balance in different tissues of the tomato plant in relation to nitrate, urea or ammonium nitrate. Plant Physiol 42:6–14
Kraus EJ, Kraybill HR (1918) Vegetation and reproduction with special reference to the tomato. Oreg Agric Exp Stn Bull 149
Lovell PH, Illsley A, Moore KG (1974) Endogenous sugar levels and their effects on root formation and petiole yellowing of detached mustard cotyledons. Physiol Plant 31:231–236
Martin SM, Rose D (1976) Growth of plant cell (Ipomea) suspension cultures at controlled pH levels. Can J Bot 54:1264–1270
Moore KG, Cobb A, Lovell PH (1972) Effects of sucrose on rooting and senescence in detachedRaphanus sativus L. cotyledons. J Exp Bot 23:65–74
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nanda KK, Jain MK (1972) Utilization of sugars and starch as carbon sources in the rooting of etiolated stem segments ofPopulus nigra. New Phytol 71:825–828
Reid ME (1924) Relation of kind of food reserves to regeneration in tomato plants. Bot Gaz 77:103–110
Reid ME (1924) Quantitative relations of carbohydrates to nitrogen in determining growth responses in tomato cuttings. Bot Gaz 77:404–418
Reinhold L, Eshhar Z (1968) Transport of 3-O-methylglucose into and out of storage cells ofDaucus carota. Plant Physiol 43:1029–1030
Schrader AL (1924) The relation of chemical composition of the regeneration of roots and tops on tomato cuttings. Proc Am Soc Hortic Sci 21:187–194
Starring CC (1923) Influence of the carbohydrate-nitrate content of cuttings upon the production of roots. Proc Am Soc Hortic 20:288–292
Street HE, Sheat DEG (1958) The absorption and availability of nitrate and ammonia. In: Encyclopedia of plant physiology, vol 8, pp 150–165
Thimann KV, Poutasse E (1941) Factors affecting root formation ofPhaseolus vulgaris. Plant Physiol 16:585–594
Van Overbeek J, Gordon SA, Gregory LE (1945) An analysis of the function of the leaf in the process of root formation in cuttings. Am J Bot 33:100–107
Welander TA (1976) Effects of nitrogen, sucrose, IAA, and kinetin on explants ofBeta vulgaris grown in vitro. Physiol Plant 36:7–10
Welander TA (1978) Influence of nitrogen and sucrose in the medium and of irradiance of the stock plants on root formation inPelargonium petioles grown in vitro. Physiol Plant 43:136–141
Wetherell DF, Dougall DK (1976) Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tisue. Physiol Plant 37:97–103
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Hyndman, S.E., Hasegawa, P.M. & Bressan, R.A. The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant Cell Tiss Organ Cult 1, 229–238 (1981). https://doi.org/10.1007/BF02318919
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DOI: https://doi.org/10.1007/BF02318919