Summary
Cell-free peritoneal washings (PW) were collected from mice with Ehrlich ascitic carcinoma at various time intervals after tumor transplantation and tested on the immunosuppressive activity in vitro. Two assays were used: (1) stimulation of human peripheral blood lymphocytes by phytohemagglutinin (PHA) and (2) inhibition of human leukocyte migration with PPD. Fluids collected in the early phase of tumor growth (up to day 6) were ineffective, or weakly suppressive, and those collected in advanced and terminal stages of carcinoma development, were suppressive in both tests applied.
Control cell-free peritoneal washings obtained from normal mice without the tumor exerted suppressive activity when adjusted to the same protein concentration as the PW from mice with Ehrlich carcinoma. This suggests that host's cells in tumor-bearing animals, rather than tumor cells themselves, may be responsible for the production of inhibitory substance(s).
Cell-free peritoneal washings were also tested on their ability to influence the growth of Ehrlich carcinoma cells in vitro, as measured by3H-thymidine incorporation. Samples collected from mice with advanced tumors stimulated the growth of tumor cells.
Similar content being viewed by others
References
Badger AM, Cooperband SR, Merluzzi VJ, Glasgow AH (1977) Immunosuppressive activity of ascitic fluid from patients with cancer metastatic to the peritoneum. Cancer Res 37:1220–1226
Berendt MJ, North RJ (1980) T-cell-mediated suppression of anti-tumor immunity. An explanation for progressive growth of an immunogenic tumor. J Exp Med 151:69–80
Bichel P (1972) Specific growth regulation in three ascitic tumors. Eur J Cancer 8:167–173
Bøyum A (1968) Separation of leukocytes from blood and bone marrow. Scand J Lab Invest [Suppl 97] 21:77–89
Cooperband SR, Nimberg R, Schmid K, Mannick JA (1976) Humoral immunosuppressive factors. Transplant Proc 8:225–242
Fujimoto S, Greene ME, Sehon AH (1976). Regulation of the immune response to tumor antigens. I. Immunosuppressor cells in tumor-bearing hosts. J Immunol 116:791–799
Gabrilovac J, Pachmann K, Thierfelder S (1981). Suppressive activity of cell-free ascites obtained from murine tumors. Soluble Fc receptor as a possible active agent. Res Exp Med 178:131–140
Gabrilovac J, Čepelak I, Boranić M (1981) A newβ-protein fraction in Ehrlich ascitic fluid. Periodicum Biologorum 83:283–289
Goren R, Nelken D (1980) Normal immunosuppressive protein. Isolation of a glycoprotein active fraction. Immunology 39:305–309
Gresser I, Vignaux F, Maury C, Lindahl P (1975). Factor(s) from Ehrlich ascites cells responsible for delayed rejection of skin allografts in mice and its assay on lymphocytes in vitro. Proc Soc Exp Biol Med 149:83–88
Jurin M, Plavšić B (1978) Dynamics of immunity in syngeneic lymphoma-bearing mice. Eur J Cancer 14:653–659
Hršak I (1971) Immunological reactivity of mice injected with leukaemic cells. In: Lindahl-Kiessling K, Alm G, Hanna MG, Jr (eds) Morphological and functional aspects of immunity. New York, Plenum Press, pp 533–538
Hršak I, Marotti T (1973) Immunosuppression mediated by Ehrlich ascites fluid. Eur J Cancer 9:717–724
Hršak I, Marotti T (1974) Mode of immunosuppressive action of Ehrlich ascitic fluid. J Natl Cancer Inst 53:1113–1119
Kirchner H, Chused TM, Herberman RB, Holden HT, Lavrin DH (1974) Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Molony sarcoma virus. J Exp Med 139:1473–1487
Manor Y, Treves AJ, Cohen IR, Feldman M (1976) Transition from T-cell protection to T-cell enhancement during tumor growth in an allogeneic host. Transplantation 22:360–366
Marotti T, Hršak I (1981) Attempt to define immunosuppressive principle(s) in ascitic fluid of Ehrlich carcinoma. Periodicum Biologorum 83:289–295
Mizel SB, De Larko JE, Todaro GJ, Ferrar WL, Hifliker ML (1980) In vitro production of immunosuppressive factors by murine sarcoma virus-transformed mouse fibroblasts. Proc Natl Acad Sci US 77:2205–2208
Myking AO, Abro A (1974) Particles similar to mouse leukemia virus in Ehrlich ascites carcinoma and in reticulum cell neoplasmas type B. Acta Pathol Microbiol Scand [A] 82:571–577
Padarathsingh ML, Dean JH, Jerrells TR, McCoy JL, Lewis DD, Northing JW (1979) Evidence for and characterization of suppressor cells in BALB/c mice bearing ADJ-PC 5 plasmocytomas. J Natl Cancer Inst 62:1235–1241
Shields R (1978) Growth factors for tumors. Nature 272:670–671
Søberg M, Bendixen B (1967) Human lymphocyte migration as a parameter of hypersensitivity. Acta Med Scand 181:247–256
Thorpe PE, Knight SC (1974) Microplate culture of mouse lymph node cells. I. Quantitation of responses to allogeneic lymphocytes, endotoxin and phytomitogens. J Immunol Methods 5:387–404
Ting CC, Tsai SC (1977) Host control of tumor growth. Science 197:571–573
Wolfson WQ, Cohn C, Calvary E, Ichiba F (1948) Study in serum proteins. V. A rapid procedure for the estimation of total protein, true albumin, total globulin, beta globulin and gamma globulin in 1.0 ml of serum. Am J Clin Pathol 18:723–730
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gabrilovac, J., Benković, B., Burek, B. et al. Immunoregulatory activity of cell-free peritoneal washings of mice with Ehrlich ascitic carcinoma. Res. Exp. Med. 180, 147–154 (1982). https://doi.org/10.1007/BF01851053
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01851053