Theoretical and Applied Genetics

, Volume 88, Issue 6–7, pp 685–690

Phylogeny of tall fescue and related species using RFLPs

  • W. W. Xu
  • D. A. Sleper

DOI: 10.1007/BF01253971

Cite this article as:
Xu, W.W. & Sleper, D.A. Theoret. Appl. Genetics (1994) 88: 685. doi:10.1007/BF01253971


The wild species of tall fescue (Festuca arundinacea var.genuina Schreb.) represent a wide range of genetic variation and constitute potential germplasm for tall fescue improvement. Our objective was to evaluate genome specificity of the previously-identified DNA probes and to examine the phylogenetic relationship of tall fescue with six related species by using RFLP data. A total of 29 DNA probes from aPstI-genomic library of tall fescue were hybridized toEcoRI-orHindIII-digested DNA of 32 plants from sixFestuca species and fromLolium perenne L. Fifteen probes hybridized to all seven species. The remaining 14 probes showed differential hybridization patterns (i.e., ±), especially at the diploid and tetraploid levels. This hybridization pattern reflected genome divergence in these species. The DNA probes will be useful markers in breeding programs involving interspecific and intergeneric hybridization. Cluster analyses were performed using the average genetic distances calculated with the RFLP data from 53 probe-enzyme combinations. Generally, genotypes from the same species were grouped in the same cluster. These data indicated that tall fescue has a close relationship withF. pratensis Huds. (diploid),F. arundinacea var.glaucescens Boiss. (tetraploid), andL. perenne L. (diploid) and thatFestuca pratensis andL. perenne had the closest degree of relationship.

Key words

Festuca arundinacea Lolium perenne Genome-specific probes Grass breeding 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • W. W. Xu
    • 1
  • D. A. Sleper
    • 1
  1. 1.Department of AgronomyUniversity of Missouri-ColumbiaColumbiaUSA

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