Antonie van Leeuwenhoek

, Volume 64, Issue 3–4, pp 253–260 | Cite as

Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test

Proposal for the creation of a new genus Paenibacillus
  • Carol Ash
  • Fergus G. Priest
  • M. David Collins


Comparative 16S rRNA sequence analysis has demonstrated that the genusBacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprisingBacillus polymyxa and close relatives is phylogenetically so removed fromBacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genusPaenibacillus. The genusPaenibacillus can be readily distinguished from otherBacillus groups using a battery of phenotypic characters and a highly specific gene probe based on 16S rRNA.

Key words

Bacillus Paenibacillus rRNA probe 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ash C, Farrow JAE, Wallbanks S & Collins MD (1991) Phylogenetic heterogeneity of the genusBacillus revealed by comparative analysis of small subunit — ribosomal RNA sequences. Lett. Appl. Microbiol. 13: 202–206Google Scholar
  2. Claus D & Berkeley RCW (1986)Bacillus Cohn 1872. In: Sneath PHA (Ed) Bergey's Manual of Systematic Bacteriology, Vol 2 (pp 1105–1139). Baltimore, The Williams and Wilkins Co.Google Scholar
  3. Fitch WM & Margoliash E (1967) Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155: 279–284Google Scholar
  4. Fox GE, Pechman KR & Woese CR (1977) Comparative cataloging of 16S ribosomal ribonucleic acid: molecular approach to prokaryotic systematics. Int. J. Syst. Bacteriol. 27: 44–57Google Scholar
  5. Hutson RA, Thompson DE & Collins MD (1993) Genetic interrelationships of saccharolyticClostridium botulinum types B, E and F and related clostridia as revealed by small — subunit rRNA gene sequences. FEMS Microbiol. Lett. 108: 103–110Google Scholar
  6. Lawson PA, Gharbia SE, Shah HA & Clark DR (1989) Recognition ofFusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restricted patterns. FEMS Microbiol. Lett. 65: 41–46Google Scholar
  7. Marshall BJ & Ohye DF (1966)Bacillus macquariensis n.sp., a psychrotrophic bacterium from sub-antarctic soil. J. Gen. Microbiol. 44: 41–46Google Scholar
  8. Nakamura LK (1984a)Bacillus amylolyticus sp. nov., nom. rev.,Bacillus lautus sp. nov., nom. rev.,Bacillus pabuli sp. nov., nom. rev., andBacillus validus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 34: 224–226Google Scholar
  9. Nakamura LK (1984b)Bacillus pulvifaciens sp. nov., nom. rev. Int. J. Syst. Bacteriol. 34: 410–413Google Scholar
  10. Pichinoty F, Waterbury JB, Mandel M & Asselineau J (1986)Bacillus gordonae sp. nov., une nouvelle espèce appartenant au second groupe morphologique, dégradant divers composés aromatiques. Ann. Inst. Pasteur/Microbiol. 137A: 65–78Google Scholar
  11. Seldin L, Van Elsas JD & Penido EGC (1984)Bacillus azotofixans sp. nov., a nitrogen-fixing species from Brazilian soils and grass roots. Int. J. Syst. Bacteriol. 34: 451–456Google Scholar
  12. Stackebrandt E, Ludwig W, Weizenegger, Dorn S, McGill TJ, Fox GE, Woese CR, Schubert W & Schleifer KH (1987) Comparative 16S rRNA oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. J. Gen. Microbiol. 133:2523–2529Google Scholar
  13. Wisotzkey JD, Jurtshuk P, Fox GE, Deinhard G & Poralla K (1992) Comparative sequence analyses on the 16S rRNA (rDNA) ofBacillus acidocaldarius, Bacillus acidoterrestris andBacillus cycloheptanicus and proposal for creation of a new genus,Alicyclobacillus gen. nov. Int. J. Syst. Bacteriol. 42: 263–269Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Carol Ash
    • 1
  • Fergus G. Priest
    • 2
  • M. David Collins
    • 1
  1. 1.Department of Microbiology, Agricultural and Food Research CouncilInstitute of Food ResearchReadingUK
  2. 2.Department of Biological SciencesHeriot-Watt UniversityRiccarton EdinburghUK

Personalised recommendations