Abstract
Rifampicin-nalidixic acid resistant mutants of a plant growth promotingPseudomonas sp., strain PsJN, were evaluated for their ability to stimulate in vitro growth of potato. Two mutant strains, MFE (a consistent growth promoter), and IIM15 (an inconsistent growth promoter), were selected for root colonization study. Root colonization of potato plants was consistently greater with MFE than with IIM15. The population density of indigenous bacteria on the root surface of potato plants inoculated with strain MFE was significantly lower as compared to non-bacterized controls and to the plants bacterized with strain IIM15. Soil sterilization did not affect plant growth in any of the treatments. Bacterization of seed tubers with strain MFE stimulated plant emergence and root development in the field, during the first two weeks after planting. Bacterized plants also formed stolons and tubers earlier and had increased yields of commercial size tubers (≥55 mm) as compared to non-bacterized controls. Root colonization by strain MFE was positively correlated with plant growth stimulation.
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References
Bahme J B, and Schroth M N 1987 Spatial-temporal colonization patterns of a rhizobacterium on underground organs of potato. Phytopatholgy 77, 1093–1100.
Baker R 1968 Mechanisms of biological control of soilborne pathogens. Annu. Rev. Phytopathol. 6, 263–294.
Baker A W and Schippers B 1987 Microbial cyanide production in the rhizosphere in relation to potato yield reduction andPseudomonas spp.-mediated growth stimulation. Soil Biol. Biochem. 19, 451–457.
Bakker P A H M, Bakker A W, Geels F P, Lamers J G and Schippers B 1989 Increase of potato tuber yield in short rotations of potato by seed tuber treatments with fluorescentPseudomonas spp.In Effects of Crop Rotation on Potato Production in Temperate zones. Eds. J Vos, C D Van Loon and G J Bollen. pp 163–170. Kluwer Academic Publishers Dordrecht, The Netherlands.
Bakker P A H M, Bakker A W, Marugg J D, Weisbeek P J and Schippers B 1987 A bioassay for studying the role of siderophores in potato growth stimulation byPseudomonas spp. in short potato rotation. Soil Biol. Biochem. 19, 443–450.
Brown M E 1972 Plant growth substances produced by microorganisms of soil and rhizosphere. J. Appl. Bacteriol. 35, 443–451.
Brown M E 1974 Seed and root bacterization. Annu. Rev. Phytopathology 12, 181–197.
Burr T J and Caesar A 1984 Beneficial plant bacteria. Crit. Rev. Plant Sci. 2, 1–20.
Burr T J, Schroth M N, and Suslow T V 1978 Increased potato yields by treatment of seed pieces with specific strains ofPseudomonas fluorescens andP. putida. Phytopathology 68, 1377–1383.
Davison J 1988 Plant beneficial bacteria. Biotechnology 6, 282–286.
Eklund E 1970 Secondary effects of some pseudomonads in the rhizoplane of peat-grown cucumber plants. Acta Agric. Scandinavica Suppl. 17, 1–57.
Frommel M I, Nowak J and Lazarovits G 1991 Growth enhancement and developmental modifications of in vitro growth potato (Solanum tuberosum spp.tuberosum) as affected by a non-fluorescentPseudomonas sp. Plant Physiol. 96, 928–936.
Howie W J and Echandi E 1983 Rhizobacteria: Influence of cultivar and soil type on plant growth and yield of potato. Soil Biol. Biochem. 15, 127–132.
Kapulnik Y 1991 Plant-growth-promoting rhizobacteria.In Plant Roots The Hidden Half. Eds. Y Waisel, A Eshel and U Kafkafi. pp 719–729. Marcel Dekker, New York.
Kavimandan S K and Gaur A C 1971 Effect of seed inoculation withPseudomonas sp. on phosphate uptake and yield sof maize. Curr. Sci. 40, 439–440.
King E O, Ward M K and Raney D E 1954 Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44, 301–307.
Klein D A, Salzwedel J L and Dazzo F B 1990 Microbial colonization of plant roots.In Biotechnology of Plant-Microbe Interactions. Eds. J P Nakas and C Hagedorn. pp 189–225. McGraw-Hill, NY.
Kloepper J W, Scher F M, Laliberté M, and Tipping B 1986 Emergence-promoting rhizobacteria: Description and implications for agriculture.In Iron, Siderophores and Plant Diseases. Ed. T R Swimburne. pp 155–164. Plenum, NY.
Kloepper J W, Scher F M, Laliberté M and Zaleska I 1985 Measuring the spermosphere colonizing capacity (spermosphere competence) of bacterial inoculants. Can. J. Microbiol. 31, 926–929.
Kloepper J W and Schroth M N 1978 Plant growth-promoting rhizobacteria on radishes. Proc. 4th Int. Conf. Plant Path. Bact. Angers. pp 879–882.
Kloepper J W and Schroth M N 1981 Relationship of in vitro antibiosis of plant growth promoting rhizobacteria to plant growth and the displacement of root microflora. Phytopathology 71, 1020–1024.
Kloepper J W, Schroth M N and Miller T D 1980 Effect of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology 70, 1078–1082.
Kloepper J W, Zablotowicz R M, Tipping E M and Lifshitz R 1991 Plant growth promotion mediated by bacterial rhizosphere colonizers.In The Rhizosphere and Plant Growth. Eds. D L Keister and P B Cregan. pp 315–326. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Parke J L 1991 Root colonization by indigenous and introduced microorganisms.In The Rhizosphere and Plant Growth. Eds. D L Keister and P B Cregan. pp 33–42. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Reddy M S 1991 Biological control of plant diseases.In Proceedings of the Workshop on Biological Control of Pests in Canada, October, 1990. Ed., A S McClay. pp 33–41. Alberta Environmental Centre, Vegerville, Canada.
Sneh B, Dupler M, Elad Y and Baker R 1984 Chlamydospore germination ofFusarium oxysporum f. sp.cucumerinum as affected by fluorescent and lytic bacteria from aFusarium suppressive soil. Phytopathology 74, 1115–1124.
Suslow T V 1982 Role of root colonizing bacteria on plant growth.In Phytopathogenic Prokaryotes. Eds. M S Mount and G H Lacy. pp 187–223. Academic Press, London, UK.
Suslow T V and Schroth M N 1982 Rhizobacteria on sugar beets: Effects of seed application and root colonization on yield. Phytopathology 72, 199–206.
Whipps J M and Lynch J M 1986 The influence of the rhizosphere on crop productivity. Adv. Microbiol. Ecol. 9, 187–244.
Xu G W and Gross D C 1986 Field evaluation of the interactions among fluorescent pseudomonads,Erwinia carotovora, and potato yields. Phytopathology 76, 423–430.
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Frommel, M.I., Nowak, J. & Lazarovits, G. Treatment of potato tubers with a growth promotingPseudomonas sp.: Plant growth responses and bacterium distribution in the rhizosphere. Plant Soil 150, 51–60 (1993). https://doi.org/10.1007/BF00779175
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DOI: https://doi.org/10.1007/BF00779175