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Glycoconjugate Journal

, Volume 11, Issue 6, pp 527–532 | Cite as

Effects of natural complex carbohydrate (citrus pectin) on murine melanoma cell properties related to galectin-3 functions

  • H. Inohara
  • A. Raz
Lectin Papers

Abstract

Citrus pectin (CP) and pH-modified citrus pectin (MCP) are highly branched and non-branched complex polysaccharides, respectively, rich in galactoside residues, capable of combining with the carbohydrate-binding domain of galectin-3. We reported previously that intravenous injection of B16-F1 murine melanoma cells with CP or MCP into syngeneic mice resulted in a significant increase or decrease of lung colonization, respectively (Platt D, Raz A (1992)J Natl Cancer Inst84:438–42). Here we studied the effects of these polysaccharides on cell-cell and cell-matrix interactions mediated by carbohydrate-recognition. MCP, but not CP, inhibited B16-F1 melanoma cells adhesion to laminin and asialofetuin-induced homotypic aggregation. Both polysaccharides inhibited anchorage-independent growth of B16-F1 cells in semisolid medium, i.e. agarose. These results indicate that carbohydrate-recognition by cell surface galectin-3 may be involved in cell-extracellular matrix interaction and play a role in anchorage-independent growth as well as thein vivo embolization of tumour cells.

Keywords

galectin-3 citrus pectin melanoma 

Abbreviations

CP

natural citrus pectin

MCP

pH-modified CP

EHS

Englebreth-Holm Swarm

CMF-PBS

Ca2+-and Mg2+-free phosphate-buffered saline, pH 7.2

HRP

horseradish peroxidase

ABTS

2,2′-azino-di(3-ethylbenzthiazoline sulfonic acid

DMEM

Dulbecco's modified Eagle's minimal essential medium

BSA

bovine serum albumin

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • H. Inohara
    • 1
  • A. Raz
    • 1
  1. 1.Cancer Metastasis ProgramMichigan Cancer FoundationDetroitUSA

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