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Molecular and General Genetics MGG

, Volume 174, Issue 1, pp 59–66 | Cite as

Overlapping sequences of Klebsiella pneumoniae nif DNA cloned and characterised

  • Frank C. Cannon
  • Gerard E. Riedel
  • Fredrick M. Ausubel
Article

Summary

A HindIII (17.0 kb) and an EcoRl restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonculease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb.

Keywords

Gene Cluster Structural Gene Southern Blotting Restriction Fragment Klebsiella 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Frank C. Cannon
    • 1
  • Gerard E. Riedel
    • 2
  • Fredrick M. Ausubel
    • 2
  1. 1.A.R.C. Unit of Nitrogen FixationUniversity of SussexBrightonEngland
  2. 2.Department of BiologyHarvard UniversityCambridgeUSA

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