Abstract
Comparative studies of the toxicity of Cd, Co, Cu, and Ni to walled (UTCC 11) and wall-less (UTCC 12) strains of Chlamydomonas reinhardtii were made in order to test the hypothesis that the cell wall affords some protection against metal toxicity. The wall-less strain was consistently more sensitive than the walled strain to all four metals, indicating that the cell wall plays a role in conferring metal tolerance. Between-strain differences were most striking for Cu and for Co. The effect of hydrogen ion concentration (pH 5 and 6.8) on metal toxicity was also determined for the two strains. Having established that both strains grew equally well at pH 5 or 7 in the absence of added metal, it was necessary to correct for the changes in metal speciation due to pH in the medium used for the tests. Speciation of each metal at each pH was determined by mathematical (GEOCHEM) modeling of the medium and the calculated free (ionic) metal concentration was used to express toxicity. In addition, the concentration of ionic metal that reduced final cell density to 30% of that in control solution (EC30) was used as an indicator of relative metal toxicity. For both strains, all metals were less toxic at pH 5 than at pH 7, supporting previous observations. The results are discussed in terms of the possible mechanisms by which the cell wall could protect the cell from metal toxicity, and the relevance of the results to more general considerations of metal tolerance mechanisms in plants.
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References
Bates SS, Tessier A, Campbell PGC, Buffle J (1982) Zinc adsorption and transport by Chlamydomonas variabilis and Scenedesmus subspicatus (Chlorophyceae) grown in semi-continuous culture. J Phycol 18:521–529
Bruce RD, Versteeg DJ (1992) A statistical procedure for modelling continuous toxicity data. Environ Toxicol Chem 11:1485–1494
Button KS, Hostetter HP (1977) Copper sorption and release by Cyclotella meneghiniana (Bacillariophyceae) and Chlamydomonas reinhardtii (Chlorophyceae). J Phycol 13:198–202
Cain JR, Allen RK (1980) Use of a cell wall-less mutant strain to assess the role of the cell wall in Cd and Hg tolerance by Chlamydomonas reinhardtii. Bull Environ Contam Toxicol 25:797–801
Campbell PGC, Stokes PM (1985) Acidification and toxicity of metals to aquatic biota. Can J Fish Aquat Sci 42:2034–2049
Crist RH, Martin JR, Guptill PW, Eslinger JM (1990) Interaction of metals and protons with algae. 2. Ion exchange in adsorption and metal displacement by protons. Environ Sci Technol 24:337–342
—, Oberholser K, Shank N, Nguyen M (1981) Nature of bonding between metallic ions and algal cell walls. Environ Sci Technol 15:1212–1217
Cumming JR, Taylor GJ (1990) Mechanisms of metal tolerance in plants: Physiological adaptations for exclusion of metals from the cytoplasm: In: Alscher RG, Cumming JR (eds) Stress responses in plants: Adaptation and acclimation mechanisms. Wiley-Liss Inc, NY, p 329
Davies DR, Plaskitt A (1971) Genetical and structural analyses of cell-wall formation in Chlamydomonas reinhardi. Genet Res 17:33–43
De Haan H (1984) Effects of metal speciation on growth of phytoplankton with special reference to iron. Hydrobiological Bulletin 18:85–94
Foster PL (1977) Copper exclusion as a mechanism of heavy metal tolerance in green alga. Nature 269:322–323
Gadd GM, Griffiths AJ (1978) Microorganisms and heavy metal toxicity. Microbial Ecol 4:303–317
Glass SA, Bostrom A, Mitchell RR, Kuo J (1992) SigmaStat: Statistical software for working scientists. Jandel Scientific, San Rafael, CA
Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM (1966) Hydrogen ion buffers for biological research. Biochemistry 5:467–477
Greger M, Tillberg J-E, Johansson M (1992) Aluminum effects on Scenedesmus obtusiusculus with different phosphorus status. I. Mineral uptake. Physiol Plant 84:193–201
Harrison GI, Campbell PGC, Tessier A (1986) Effects of pH changes on zinc uptake by Chlamydomonas variablis grown in batch culture. Can J Fish Aquat Sci 43:687–693
Huntsman SA, Sunda WG (1980) The role of trace metals in regulating phytoplankton growth with emphasis on Fe, Mn, and Cu. In: Morris I (ed) The physiological ecology of phytoplankton. University of California Press, Berkeley, CA, pp 285–328
Hyams J, Davies DR (1972) The induction and characterization of cell wall mutants of Chlamydomonas reinhardi. Mutation Res 14:381–389
Irmer U, Wachholz I, Schafer H, Lorch W (1986) Influence of lead on Chlamydomonas reinhardtii Danegard (Volvocales, Chlorophyta): Accumulation, toxicity and ultrastructural changes. Environ Exp Bot 26:97–105
Jensen TE, Rachlin JW, Vandana J, Warkentine B (1982) An x-ray energy dispersive study of cellular compartmentalization of lead and zinc in Chlorella saccharophila (Chlorophyta), Navicula incerta and Nitzschia closterium (Bacillariophyta). Environ Exp Bot 22:319–328
Kawasaki T, Moritsugu M (1987) Effect of calcium on the absorption and translocation of heavy metals in excised barley roots: Multi-compartment transport box experiment. Plant Soil 100:21–34
Kesseler H (1980) On the selective adsorption of cations in the cell wall of the green alga Valonia utricularis. Helgolander Meeresunters 34:151–158
Kuwabara JS (1986) Physico-chemical processes affecting copper, tin, and zinc toxicity to algae: a review. In: Evans LV, Hoagland KD (eds) Algal biofoulins. Elsevier, Amsterdam, pp 129–144
Macfie SM (1993) The influence of the cell wall on tolerance of four metals in the alga Chlamydomonas reinhardtii. Intl conf heavy metals in the environment, Sept 1993, Toronto, Canada, Vol 2, CEP Consultants, Edinburgh, UK, pp 50–53
Majidi V, Laude DA Jr, Holcombe JA (1990) Investigation of the metal-algae binding site with 113Cd nuclear magnetic resonance. Environ Sci Technol 24:1309–1312
Matthews TH, Doyle RD, Streips UN (1979) Contribution of peptidoglycan to the binding of metal ions by the cell wall of Bacillus subtilis. Curr Microbiol 3:51–53
Nieboer E, Richardson DHS (1980) The replacement of the nondescript term “heavy metals” by a biologically and chemically significant classification of metal ions. Environ Pollut 1:3–26
Parent L, Campbell PGC (1994) Aluminum bioavailability to the green alga Chlorella pyrenoidosa in acidified synthetic soft water. Environ Toxicol Chem 13:587–598
Payne AG (1975) Responses of the three test algae of the algal assay procedure: Bottle test. Water Res 9:P437–445
Peterson R (1982) Influence of Cu and Zn on the growth of a freshwater alga, Scenedesmus quardicauda: The Significance of chemical speciation. Environ Sci Technol 16:443–447
Rai LC, Gaur JP, Kumar HD (1981) Phycology and heavy-metal pollution. Biol Rev 56:99–151
Sueoka N (1960) Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 46:83–91
Tarmohamed Y (1990) The role of the cell wall in nickel and cadmium toxicity and uptake in two strains of Chlamydomonas reinhardtii. MSc thesis, Department of Botany and Environmental Science, University of Toronto, Canada
Vallee B, Ulmer DD (1986) Biochemical effects of mercury, cadmium and lead. Ann Rev Biochem 41:91–128
Van Cutsem P, Gillet C (1982) Activity coefficients and selectivity values of Cu++, Zn++, and Ca++ ions adsorbed in the Nitella flexilis L. cell wall during triangular ion exchanges. J Exp Bot 33:847–853
Voigt J (1988) The lithium-chloride-soluble cell-wall layers of Chlamydomonas reinhardtii contain several immunologically related glycoproteins. Planta 173:373–384
Xue H-B, Stumm W, Sigg L (1988) The binding of heavy metals to algal surfaces. Water Res 22:917–926
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Macfie, S.M., Tarmohamed, Y. & Welbourn, P.M. Effects of cadmium, cobalt, copper, and nickel on growth of the green alga Chlamydomonas reinhardtii: The influences of the cell wall and pH. Arch. Environ. Contam. Toxicol. 27, 454–458 (1994). https://doi.org/10.1007/BF00214835
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DOI: https://doi.org/10.1007/BF00214835