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Endogenous zinc concentrations in cysteamine-induced duodenal ulcers in the rat

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Abstract

Exogenously administered zinc compounds have been shown to possess anti-ulcer activity against a wide variety of ulcerogenic agents, both in laboratory animal models and in human peptic ulcer disease. However, a strong possibility exists that endogenous zinc may also play an important role during noxious events by various mechanisms. Therefore, the aim of this study was to focus on the changes of endogenous zinc serum and tissue concentrations in cysteamine-induced duodenal lesions. We used atomic absorption spectropho-tometry to determine the tissue and serum concentrations of zinc in normal (control) rats and those with cysteamine-induced duodenal ulcers. The results obtained in this study indicated that the onset, development and spontaneous healing of ulcer lesions were associated with certain shifts in zinc serum and tissue concentrations. Prior to ulcer formation, a significant increase was noted in serum zinc values. With the onset of duodenal lesions, zinc serum concentrations significantly decreased, while there was a significant increase in duodenal tissue concentrations when compared to healthy control animals. Zinc tissue concentrations decreased and returned to starting values by the end of the first week of spontaneous healing. This decrease in zinc tissue concentration corresponded to the healing rate of the duodenal ulcers. Serum zinc concentrations also returned to starting values within the first week period. These observations indicate and confirm that zinc could play an important role in duodenal ulcer disease and represent a natural defense system in the body.

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References

  • Blanusa M. Breski D. 1981 Comparison of dry and wet ashing procedure for cadmium and iron determination in biological material by atomic absorption spectrophotometry. Talanta 28, 681–684.

    Google Scholar 

  • Borovansky J, Riley PA. 1989 Cytotoxicity of zinc in vitro. Chem-Biol interact 69, 279–291.

    Google Scholar 

  • Cho CH. 1989 Current views of zinc as a gastrohepatic protective agent. Drug Dev Res 17, 185–197.

    Google Scholar 

  • Chu CH. Ogle CW. 1978 A correlative study of the antiulcer effects of zinc sulphate in stressed rats. Eur J Pharmacol 48, 97–105.

    Google Scholar 

  • Cho CH, Ogle CW, Dai S. 1976 Acute gastric ulcer formation in response to electrical vagal stimulation in rats. Eur J Pharmacol 35, 215–219.

    Google Scholar 

  • Cho CH, Chen BW, Poon YK. et al. 1989 Dual effect of zinc sulphate on ethanol-induced gastric injury in rats: possibly mediated by an action on mucosal blood flow. J Pharm Pharmacol 41, 685–689.

    Google Scholar 

  • Cho CH, Hui WM, Chen BW, et al. 1992 The cytopro-tective effect of zinc-L-carnosine on ethanol-induced gastric gland damage in rabbits. J Pharm Pharmacol 44, 364–365.

    Google Scholar 

  • Chvapil M, Zukowski CF. 1975 New concept on the mechanism of the biological effect of zinc. In: Poires WJ, ed. Clinical Application of Zinc Therapy. Springfield: Thomas.

    Google Scholar 

  • Cordova A. 1994 Zinc content in selected tissues in streptozocin-diabetic rats after maximal exercise. Biol Trace Elem Res 42, 209–216.

    Google Scholar 

  • Elinder CG. 1986 Zinc. In: Friberg L, Nordberg GF, Vouk VB, eds. Handbook on Toxicology of Metals. Amsterdam: Elsevier.

    Google Scholar 

  • Esplugues JV, Bulbena O, Escolar G, Marti-Bonmati E, Esplugues J. 1985 Effects of zinc acexamate on gastric mucosal resistance factors. Eur J Pharmacol 109, 145–151.

    Google Scholar 

  • Falchuk KH, Hilt KL, Vallee BL. 1988 Zinc in biological samples by atomic absorption spectrophotometry. Methods Enzymol 158, 422–434.

    Google Scholar 

  • Ito M, Inaguma K, Suzuki Y, Segami T, Suzuki Y. 1995 Healing-promoting action of the zinc-cimetidine complex on acetic acid-induced gastric ulcers in rats. Jpn J Pharmacol 68, 287–295.

    Google Scholar 

  • Lee PWR, Green MA, Long WB, Gill W. 1976 Zinc and wound healing. Surg Gynecol Obstet 143, 549–554.

    Google Scholar 

  • Lindeman RD, Bottomley RC, Cornelison RL Jr, Jacobs J. 1972 Influence of acute injury on zinc metabolism in man. J Lab Clin Med 79, 452–457.

    Google Scholar 

  • Lloris JM, Esplugues JV, Sarria B, et al. 1988 Effect of zinc sulphate on gastric mucosal blood flow and gastric emptying in the rat. J Pharm Pharmacol 40, 60–61.

    Google Scholar 

  • Momcilovic B, Belonje B, Sham BG. 1975 Effect of the matrix of the standard on results of atomic absorption spectrophotometry of zinc in serum. Clin Chem 21, 588–590.

    Google Scholar 

  • Navarro C, Escolar G, Baños JE, Casanovas LI, Bulbena O. 1988 Effect of zinc acexamate on gastric mucosal production of prostaglandin E2 in normal and stressed rats. Prost Leucotr Essential Fatty Acids 33, 75–80.

    Google Scholar 

  • Navarro C, Ramis A, Sendros S, et al. 1990 Relationship between gastric levels and antiulcerogenic activity of zinc. Arch Int Pharmacodyn 307, 119–129.

    Google Scholar 

  • Oberlas D, Seymour JK, Lenaghan R, et al. 1971 Effect of zinc deficiency on wound-healing in rats. Am J Surg 121, 566–568

    Google Scholar 

  • Ogle CW, Lau HK. 1979 Disodium cromoglycate: its influence on gastric ulcers produced by stress in rats. IRCS Med Sci 7, 393–394.

    Google Scholar 

  • Pfeiffer CJ, Bulbena O, Esplugues JV, et al. 1987a Antiulcer and membrane stabilizing actions of zinc acexamate. Arch Int Pharmacodyn 285, 148–157.

    Google Scholar 

  • Pfeiffer CJ, Pfeiffer DC, Szabo S. 1987b Ultrastructural changes in rat duodenal mucosa associated with cysteamine-induced ulcer. Exp Mol Pathol 46, 102–113.

    Google Scholar 

  • Poires WJ. 1957 The acceleration of wound and burn healing with zinc compounds. PhD Thesis, University of Rochester, School of Medicine.

  • Poires WJ, Henzel JH, Rob CG, Strain WH. 1967 Acceleration of wound healing in man with zinc sulphate given by mouth. Lancet i, 121–124.

    Google Scholar 

  • Poulsen SS, Kirkegard P, Skov Olsen P, Christiansen J 1981 Brunner's glands of the rat during cysteamine ulceration. Scand J Gastroenterol 16, 459–464.

    Google Scholar 

  • Sanstead HH, Shepard GH. 1986 The effect of zinc deficiency on tensile strength of healing surgical incisions in the integument of the rat. Proc Soc Exp Biol Med 128, 687–689.

    Google Scholar 

  • Savlov ED, Strain WH, Huegin F. 1962 Radio zinc studies in experimental wound healing. J Surg Res 2, 209–212.

    Google Scholar 

  • Seyle H. 1956 The Stress of Life. New York: McGraw-Hill.

    Google Scholar 

  • Seyle H, Szabo S. 1973 Experimental ulcer model for production of perforating duodenal ulcers by cysteamine in the rat. Nature 244, 458–459.

    Google Scholar 

  • Szabo S. 1978 Animal model of human disease: duodenal ulcer disease. Am J Pathol 93, 273–276.

    Google Scholar 

  • Szabo S. 1985 Understanding biologic stress for study design and interpretation of results. Dig Dis Sci 12, 28S-31S.

    Google Scholar 

  • Tsuchihashi Y, Fuse Y, Sucihara H, Fugita S. 1985 Color image analysis of mucin content of Brunner's glands in cysteamine-induced duodenal ulceration. In: Kawai K, ed. Gastric Mucus Secreting Cells. Tokyo: Excerpta Medica; 19–28.

    Google Scholar 

  • Vallee BL, Falchuk KH. 1993 The biochemical basis of zinc physiology. Physiol Rev 73, 79–118.

    CAS  PubMed  Google Scholar 

  • Wong SH, Cho CH, Ogle CW. 1986 Protection by zinc sulphate against ethanol-induced ulceration: preservation of the gastric mucosal barrier. Pharmacology 33, 94–102.

    Google Scholar 

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Authors and Affiliations

  1. Department of Gastroenterology, University Hospital ‘Sestre Milosrdnice’, Medical School, University of Zagreb, Zagreb, Croatia

    Branko Troskot, Milan Dodig, Ivo Rotkvic & Marko Duvnjak

  2. PLIVA Pharmaceutical Co., Research Institute, Biomedical Department, Zagreb, Croatia

    Velimir N. Simicevic

  3. Department of Statistics, Epidemiology and Informatics, School of Public Health Andrija Stampar, Medical School, University of Zagreb, Zagreb, Croatia

    Davor Ivankovic

Authors
  1. Branko Troskot
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  2. Velimir N. Simicevic
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  3. Milan Dodig
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  4. Ivo Rotkvic
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  5. Davor Ivankovic
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  6. Marko Duvnjak
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Troskot, B., Simicevic, V.N., Dodig, M. et al. Endogenous zinc concentrations in cysteamine-induced duodenal ulcers in the rat. Biometals 9, 371–375 (1996). https://doi.org/10.1007/BF00140606

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  • DOI: https://doi.org/10.1007/BF00140606

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