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Euphytica

, Volume 88, Issue 1, pp 25–34 | Cite as

Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques

  • M. van Duren
  • R. Morpurgo
  • J. Dolezel
  • R. Afza
Article

Summary

Flow cytometry and stomata characteristics were used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata breeding clone SH-3362. Culturing shoot tips in liquid medium stipplemented both with 5.0 mM colchicine for 48 hours or 30 μM oryzalin (3,5-dinitro-N4,N-dipropylsulphate) for seven days, both in combination with 2% (v/v) DMSO, resulted in a high (23.1% and 29.1%) frequency of non-chimeric tetraploids in the fourth vegetative generation. Although mixoploidy persisted in subsequent cycles of vegetative propagation, tetraploids as identified by flow cytometry remained solid non-chimeric during two more cycles. These autotetraploids were propagated for field testing. A rough pre-selection of regenerated V4 plants based on their stomata characteristics resulted in a population in which only 56.2% of the plants were solid tetraploids. The somatic polyploidization system reported here can be utilised for banana breeding programmes.

Key words

banana breeding chimerism colchicine flow cytometry mixoploidy Musa oryzalin tetraploids 

Abbreviations

FCM

Flow Cytometry

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • M. van Duren
    • 1
  • R. Morpurgo
    • 1
  • J. Dolezel
    • 2
  • R. Afza
    • 1
  1. 1.Plant Breeding UnitFAO/IAEA Agriculture and Biotechnology LaboratorySeibersdorfAustria
  2. 2.De Montfort University Norman Borlaug Centre for Plant ScienceInstitute of Experimental BotanyOlomoucCzech Republic

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