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Production of an extracellular polysaccharide byAgrobacterium sp DS3 NRRL B-14297 isolated from soil

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Journal of Industrial Microbiology

Abstract

A bacterium isolated from soil and identified asAgrobacterium sp produced a water-soluble extracellular polysaccharide capable of producing highly viscous solutions. Gas chromatographic analysis revealed a sugar composition of glucose, galactose and mannose in the molar ratio of 7.5∶2.4∶1, together with 3.7% (w/w) pyruvic acid. Methylation analyses showed the presence of (1→3)-, (1→4)- and (1→6)-linked glucose, (1→3)- and (1→4, 1→6)-linked galactose and a small portion of (1→3)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2×106 Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55°C. Maximum yield of the polysaccharide, 20 g L−1, was reached in 48 h at 30°C incubation.

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Authors and Affiliations

  1. Oil Chemical Research, USDA, 1815 N University Street, 61604, Peoria, IL, USA

    C T Hou & W Brown

  2. Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604, Peoria, IL, USA

    J A Ahlgren & J J Nicholson

Authors
  1. C T Hou
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  2. J A Ahlgren
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  3. W Brown
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  4. J J Nicholson
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Hou, C.T., Ahlgren, J.A., Brown, W. et al. Production of an extracellular polysaccharide byAgrobacterium sp DS3 NRRL B-14297 isolated from soil. Journal of Industrial Microbiology 16, 129–133 (1996). https://doi.org/10.1007/BF01570073

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  • DOI: https://doi.org/10.1007/BF01570073

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