Abstract
An efficient adventitious shoot regeneration system was developed for pear (Pyrus communis L.), using leaves from in vitro proliferating shoots. Under optimal conditions, bud regeneration frequencies of ‘Comice’, ‘Passe-Crassane’, ‘Williams’ and ‘Conference’ ranged from 60% to 97%, with the mean number of shoots per regenerating leaf ranging from 3.2 to 6.6. Despite the great variability in responses of the different cultivars, in general an initial dark exposure of at least 20 days was required. Ammonium and total nitrogen proved to play an essential role: intermediate NH4 + concentrations were suitable for regeneration. The balance between NH4 + and NO3 - also influenced regeneration; optimal regeneration occured on media with a 1:3 NH4 +/NO3 - ratio. TDZ at 1 μM was less efficient than higher concentrations, whatever the NAA level. Finally, length and growth regulator composition of the two phases (induction and expression) influenced the regeneration rate of ‘Conference’.
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Abbreviations
- BA:
-
6-benzyladenine
- EDFS:
-
ethylenediamine-tetraacetic acid ferric-sodium salt
- IBA:
-
4-indole-3yl-butyric acid
- NAA:
-
α-naphthaleneacetic acid
- TDZ:
-
thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)
References
Abu-Qaoud HA (1989) In vitro separation of chimeral pears into their component genotypes. PhD Thesis, University of Illinois, Urbana, Illinois: 105 p
Abu-Qaoud HA, Skirvin RM & Chevreau E (1990) In vitro separation of chimeral pears into their component genotypes. Euphytica 48: 189–196
Arnaud Y, Al Maari K, Fouret Y & Larrieu C (1987) Les régulateurs de croissance exogènes et endogènes: outils de la micropropagation et marqueurs de l'état physiologique chez les ligneux (in French). Annales de 2ème Colloque sur les Substances de Croissance et leurs Utilisations en Agriculture 3: 101–110
Chevreau E, Skirvin RM, Abu-Qaoud HA, Korban SS & Sullivan JG (1989) Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro. Plant Cell Rep. 7: 688–691
Chevreau E, Thibault B & Arnaud Y (1991) Micropropagation of pear. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry. Springer-Verlag (in press)
Christianson ML & Warnick DA (1988) Organogenesis in vitro as a developmental process. HortScience 23: 515–519
Druart P (1980) Plantlet regeneration from root callus of different Prunus species. Scientia Hortic. 12: 339–342
Dufour M (1990) Improving yield of adventitious shoots in apple. Acta Hortic. 280: 51–60
Fasolo F, Zimmerman RH & Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tiss. Org. Cult. 16: 75–87
Heller R (1977) Abrégé de physiologie végétale, Tome 1 nutrition (in French). Masson, Paris
James DJ, Passey AJ & Rugini E (1988) Factors affecting high frequency plant regeneration from apple leaf tissues cultured in vitro. J. Plant Physiol. 132: 148–154
Matsuta N & Hirabayashi T (1989) Embryogenic cell lines from somatic embryos of grape (Vitis vinifera L.). Plant Cell Rep. 7: 684–687
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Ochatt SJ & Caso OH (1986) Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var. Pyraster L.). J. Plant Physiol. 122: 243–249
Ochatt SJ & Power JB (1988) Plant regeneration from mesophyll protoplasts of Williams' Bon Chrétien (syn. Bartlett) pear (Pyrus communis L.). Plant Cell Rep. 7: 587–589
Predieri S & Fasolo Fabbri Malavasi F (1989) High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumila Mill.). Plant Cell Tiss. Org. Cult. 17: 133–142
Predieri S, Fasolo Fabbri Malavasi F, Passey AJ, Ridout MS & James DJ (1989) Regeneration from in vitro leaves of ‘Conference’ and other pear cultivars (Pyrus communis). J. Hortic. Sci. 64: 553–559
Quoirin M, Lepoivre P & Boxus P (1977) Un premier bilan de dix années de recherche sur les cultures de méristèmes et la multiplication in vitro de fruitiers ligneux (in French). Compte rendu des recherches, Station des Cultures Fruitières et Maraîchères de Gembloux 1976–1977: 93–117
Sokal RR & Rohlf FJ (1969) Biometry. The Principles and Practice of Statistics in Biological Research (pp 559–560). WH Freeman, San Francisco
Stamp JA, Colby SM & Meredith CP (1990) Direct shoot organogenesis and plant regeneration from leaves of grape (Vitis spp.). Plant Cell Tiss. Org. Cult. 22: 127–133
Suezawa K, Matsuta N, Omura M & Yamaki S (1988) Plantlet formation from cell suspensions of kiwifruit (Actinidia chinensis Planch. var. chinensis). Scientia Hortic. 37: 123–128
Swartz HJ, Bors R, Mohamed F & Naess SK (1990) The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves. Plant Cell Tiss. Org. Cult. 21: 179–184
Wanas WH, Callow JA & Withers LA (1986) Growth limitation for the conservation of pear genotypes. In: Withers LA & Alderson SPG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 285–290). Butterworths, London
Welander M (1988) Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J. Plant Physiol. 132: 738–744
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Leblay, C., Chevreau, E. & Raboin, L.M. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant Cell Tiss Organ Cult 25, 99–105 (1991). https://doi.org/10.1007/BF00042180
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DOI: https://doi.org/10.1007/BF00042180