Abstract
Background
Hepatitis B virus (HBV) infection is a major health problem among hemodialysis (HD) patients. Interleukin (IL)-12 gene polymorphisms may be associated with immune response variability to recombinant HBV vaccines. The aim was to determine the correlation between IL-12 gene polymorphism and hepatitis B surface-antibody (HBs-Ab) titer in response to HBV vaccine among HD Egyptian patients.
Patients and methods
Seventy patients receiving long-term HD and 20 age-matched and sex-matched healthy controls were enrolled. All participants were non-HBV vaccinated and seronegative for HBV and HIV. Recombinant HBV vaccine was given (three-dose scheduled). Thereafter, HBs-Ab titer and IL-12 gene polymorphism were evaluated 8 weeks after the last vaccination dose.
Results
There was no response (HBs-Ab<10 μIU/ml) in 20% of HD patients and 10% of the controls. HBs-Ab titers showed no significant correlation with duration of HD, BMI, serum albumin, hemoglobin, leucocytic count, parathyroid hormone level, or IL-12 gene polymorphism. Responders to vaccination had significantly lower transferrin saturation and significantly higher levels of urea reduction ratio, Kt/V and lymphopenia. IL-12B genotype frequency was as follows: AA (58.3 vs. 55.6%), AC (37.5%) and CC (4.2 vs. 0%) in responders of either HD or control participants, respectively (P>0.05 for all).
Conclusion
There was no significant association between IL-12B gene polymorphism and HBs-Ab response in Egyptian HD patients. In HD patients, lymphocytopnea, diabetes mellitus (DM), high transferrin saturation and inefficient HD were associated with HBV vaccine hyporesponsiveness.
Article PDF
Similar content being viewed by others
References
Paniagua R, Villasis-Keever A, del Carmen Prado-Uribe M, Alcántara-Ortega G, de Leon SR, Cure-Bolt N, Rangel-Frausto S. Elevated prevalence of hepatitis B in Mexican hemodialysis patients (a multicentric survey). Arch Med Res 2010; 41:251–254.
El Makarem MA, Hamid MA, Aleem AA, Ali A, Shatat M, Sayed D, et al. Prevalence of occult hepatitis B virus infection in hemodialysis patients from Egypt with or without hepatitis C virus infection. Hepat Mon 2012; 12:253–258.
Unger J, Peters H. Hepatitis B in chronic kidney disease: moving toward effective prevention. Kidney Int 2008; 73:799–801.
Chaves SS, Daniels D, Cooper BW, Malo-Schlegel S, MacArthur S, Robbins KC, Kobetitsch JF, et al. Immunogenicity of hepatitis B vaccine among hemodialysis patients: Effect of revaccination of non-responders and duration of protection. Vaccine 2011; 29:9618–9623.
Fabrizi F, Dixit V, Martin P, Messa P. Meta-analysis: the impact of diabetes mellitus on the immunological response to hepatitis B virus vaccine in dialysis patients. Aliment Pharmacol Ther 2011; 33:815–821.
Hauser AB, Stinghen AE, Kato S, Bucharles S, Aita C, Yuzawa Y, Pecoits-Filho R. Characteristics and causes of immune dysfunction related to uremia and dialysis. Perit Dial Int 2008; 28(Supplement 3):S183.
Eleftheriadis T, Pissas G, Antoniadi G, Liakopoulos V, Stefanidis I. Factors affecting effectiveness of vaccination against hepatitis B virus in hemodialysis patients. World J Gastroenterol 2014; 20:12018–12025.
Matthias G, Martina S, Urban S, Harald K, Hans K. Defective expression of B7-2 (CD86) on monocytes of dialysis patients correlates to the uremia-associated immune defect. Kidney Int 2001; 59:1382–1389.
Wu JF, Wu TC, Chen CH, Ni YH, Chen HL, Hsu HY, Chang MH. Serum levels of interleukin-10 and interleukin-12 predict early, spontaneous hepatitis B virus e antigen seroconversion. J Gastroenterol 2010; 138:165–172.
Lin Y, Lan Y, Wan L, Lin T, Chen D, Tsai C, et al. Serological surveillance and IL-10 genetic variants on anti-HBs titers: hepatitis B vaccination 20 years after neonatal immunization in Taiwan. Clin Chim Acta 2011; 412:766–773.
Jindal K, Chan CT, Deziel C, Hirsch D, Soroka S, Tonelli M, Culleton BF. Hemodialysis adequacy in adults. J A Soc Nephrol 2006; 17(3 suppl 1): S4–S7.
Fakruddin M, Mannan KS, Chowdhury A, Mazumdar RM, Hossain MN, Islam S, Chowdhury MA. Nucleic acid amplification: alternative methods of polymerase chain reaction. J Pharm Bioallied Sci 2013; 5:245–252.
Knapp LA. Single nucleotide polymorphism screening with denaturing gradient gel electrophoresis. Methods Mol Biol 2009; 578:137–151.
Crow JF. Hardy, Weinberg and language impediments. Genetics 1999; 152:521–525.
Strano M, Colosimo BM. Logistic regression analysis for experimental determination of forming limit diagrams. Int J Mach Tools Manu 2006; 46:673–682.
Lasek W, Zagożdżon R, Jakobisiak M. Interleukin 12: still a promising candidate for tumor immunotherapy. Cancer Immunol Immunother 2014; 63:419–435.
El-Arbagy A, Kora M, El-Barbary H, Gabr H, Selim A. Prevalence of end stage renal disease in Menoufia Governorate. Nat Sci 2015; 13:154–158.
Babitt J, Lin H. Mechanisms of anemia in CKD. J Am Soc Nephrol 2012; 23:1631–1634.
Edey M, Barraclough K, Johnson D. Review article: hepatitis band dialysis. Nephrology (Carlton) 2010; 15:137–145.
Somi M, Hajipour B. Improving hepatitis B vaccine efficacy in end-stage renal diseases patients and role of adjuvants. ISRN Gastroenterol 2012; 2012:960413.
Cheng C, Huang C, Leu M, Chiang C, Wu M, Lai P. Hepatitis B vaccine in hemodialysis patients with hepatitis C infection. Vaccine 1997; 15:1353–1357.
Navarro J, Teruel J, Mateos M, Marcen R, Ortuo J. Antibody level after hepatitis B vaccination in hemodialysis patients: influence of hepatitis C virus infection. Am J Nephrol 1996; 16:95–97.
Fabrizi F, Dixit V, Martin P, Messa P. Hepatitis C virus and the immunological response to hepatitis B virus vaccine in dialysis patients: meta-analysis of clinical studies. J Viral Hepat 2011; 18:871–876.
Grzegorzewska A, Wobszal P, Mostowska A, Jagodziński PP. Antibodies to hepatitis B virus surface antigen and interleukin 12 and interleukin 18 gene polymorphisms in hemodialysis patients. BMC Nephrol 2012; 13:75.
Wang C, Tang J, Song W, Lobashevsky E, Wilson CM, Kaslow RA. HLA and cytokine gene polymorphisms are independently associated with responses to hepatitis B vaccination. Hepatology 2004; 39:978–988.
Srinivasan V, Pandi-Perumal SR, Maestroni GJ, Esquifino AI, Hardeland R, Cardinali DP. Role of melatonin in neurodegenerative diseases. Neurotox Res 2005; 7:293–318.
Eleftheriadis T, Antoniadi G, Liakopoulos V, Kartsios C, Stefanidis I. Disturbances of acquired immunity in hemodialysis patients. Semin Dial 2007; 20:440–451.
Liu JH, Liu YL, Lin HH, Yang YF, Kuo HL, Lin PW, Huang CC. Intravenous iron attenuates post vaccination anti-HBsAg titres after quadruple hepatitis B vaccination in dialysis pa-tients with erythropoietin therapy. Int J Clin Pract 2009; 63:387–393.
Dede F, Yıldız A, Aylı D, Colak N, Odabaş A, Akoğlu H, et al. Modulation of the immune response to HBV vaccination by hemodialysis membranes. Int Urol Nephrol 2010; 42:1069–1075.
Author information
Authors and Affiliations
Corresponding author
Additional information
This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Abdel-Moniem, S.M., Mohammed, M.A., Essam El-Deen, Z.M. et al. Effects of interleukin-12 gene polymorphism on response to hepatitis B vaccination among hemodialysis Egyptian patients. Egypt J Intern Med 31, 804–812 (2019). https://doi.org/10.4103/ejim.ejim_85_19
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.4103/ejim.ejim_85_19