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RT-PCR Amplification and in-silico Analysis of Water Stress-Responsive Hydrophilic TaDreb, TaDHN and TaWCS20 Genes from Triticum aestivum

  • PLANT GROWING, PLANT PROTECTION AND BIOTECHNOLOGY
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The biochemical features that govern multi-functions of boiling soluble proteins are still not well documented. In this study, we have isolated and sequenced a water stress responsive cDNA encoding boiling soluble proteins designated as TaDreb, TaDHN and TaWCS20. (TaDREB: Triticum aestivum dehydration responsive element binding, TaDHN: Triticum aestivum dehydrin, and TaWCS120: Triticum aestivum cold acclimation protein WCS120) from seedlings exposed to water stress from wheat (PBW 175) cultivar tolerant to drought stress. We described in-silico analysis and molecular modelling of TaDreb, TaDHN and TaWCS20 through motif analysis, homology search, secondary structure prediction, active site prediction and 3D structure analysis. BLAST-N analysis revealed their resemblance to boiling soluble proteins encoding genes. The physio-chemical analysis revealed that TaDreb, TaDHN and TaWCS20 are low MW, hydrophilic, disordered and stable proteins. Multiple amino acid analysis revealed the presence of conserved domains responsive to water stress. 3D modelling analysis revealed the presence of structural rich motifs like beta hairpin, beta turns and bulges in a sequence dependent manner. Based upon these findings we suggest the possible mechanism that how these boiling soluble proteins preclude the unfavourable effect of drought stress.

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Authors and Affiliations

  1. Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine (CSIR) Canal Road, 180001, Jammu, India

    Arun Dev Sharma & Gurmeen Rakhra

  2. P.G Department of Biotechnology, Lyallpur Khalsa College, 144001, Jalandhar, Punjab, India

    Dhiraj Vyas

Authors
  1. Arun Dev Sharma
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  2. Gurmeen Rakhra
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  3. Dhiraj Vyas
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Correspondence to Arun Dev Sharma.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Arun Dev Sharma, Rakhra, G. & Vyas, D. RT-PCR Amplification and in-silico Analysis of Water Stress-Responsive Hydrophilic TaDreb, TaDHN and TaWCS20 Genes from Triticum aestivum . Russ. Agricult. Sci. 47, 218–232 (2021). https://doi.org/10.3103/S1068367421030162

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  • DOI: https://doi.org/10.3103/S1068367421030162

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