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Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. under salt stress

  • Plant Industry
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Abstract

Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. with the pea rbcS gene leader sequence under chloride and sulfate salinity has been studied. Dark respiration of transgenic tomato regenerants under stress conditions has been estimated. It has been established that expression of the FeSOD1 gene in transgenic tomato plants under salinity leads to a decrease in damage of ultrastructure organization of plastids and mitochondria and affects the size and shape of their inclusions, suggesting a protective effect of this gene under salinity. The obtained experimental data show the effectiveness of using a genetic engineering strategy of redirection and subsequent accumulation of cytoplasmic Fe-dependent SOD from Arabidopsis thaliana (L.) Heynh. in chloroplasts for increasing the tolerance of crops to salt stress.

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References

  1. Mittler, R., Oxidative stress, antioxidants and stress tolerance, Trends Plant Sci., 2002, vol. 7, pp. 405–410.

    Article  CAS  PubMed  Google Scholar 

  2. Baranova, E.N. and Gulevich, A.A., Problems and perspectives of gene-engineering approach in the salt resistance, S-kh. Biol., 2006, no. 1, pp. 39–56.

    Google Scholar 

  3. Singla-Pareek, S.L., Pareek, A., and Sopory, S.K., Transgenic plants for dry and saline encironments, in Advances in Molecular Breeding Toward Drought and Salt Tolerant Crops, Springer, 2007, pp. 501–530.

    Chapter  Google Scholar 

  4. McKersie, B.D., Bowley, S.R., Harjanto, E., and Leprince, O., Water-deficit tolerance and field performance of transgenic alfalfa overexpressing superoxide dismutase, Plant Physiol., 1996, vol. 111, no. 4, pp. 1177–1181.

    CAS  PubMed Central  PubMed  Google Scholar 

  5. Badawi, G.H., Yamauchi, Y., Shimada, E., Sasaki, R., Kawano, N., and Tanaka, K., Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts, Plant Sci., vol. 166, no. 4, pp. 919–928.

  6. Roxas, V.P., Smith, R.K. Jr., Allen, E.R., and Allen, R.D., Overexpression of glutathione S-transferase/glutathioneperoxidase enhances the growth of transferase/glutathioneperoxidase enhances the growth of transgenic tobacco seedlings during stress, Nat. Biotechnol., 1997, vol. 15, pp. 988–991.

    Article  CAS  PubMed  Google Scholar 

  7. Kliebenstein, D.J., Monde, R.A, and Last, R.L., Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization, Plant Physiol., 1998, vol. 118, pp. 637–650.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Myouga, F., Hososa, C., Umezawa, T., Iizumi, H., Kuromori, T., Motohashi, R., Shono, Y., Nagata, N., Ikeuchi, M., and Shinozaki, K., A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis, Plant Cell, 2998, vol. 20, no. 11, pp. 3148–3162.

  9. Serenko, E.K., Ovchinnikova, V.N., Kurenina, L.T., Baranova, E.N., Gulevich, A.A., Maisuryan, A.N., and Kharchenko, P.N., Production of transgenic tomato plants with the Fe-dependent superoxide dismutase gene, Russ. Agric. Sci., 2009, vol. 35, no. 4, pp. 223–226.

    Article  Google Scholar 

  10. Baranova, E.N., Serenko, E.K., Balachnina, T.I., Kosobruhov, A.A., Kurenina, L.V., Gulevich, A.A., and Maisuryan, A.N., Activity of the photosynthetic apparatus and antioxidant enzymes in leaves of transgenic Solanum lycopersicum and Nicotiana tabacum plants with FeSOD1 gene, Russ. Agric. Sci., 2010, vol. 36, no. 4, pp. 242–249.

    Article  Google Scholar 

  11. Serenko, E.K., Kurenina, L.V., Gulevich, A.A., Maysurian, A.N., Baranova, E.N., Balakhnina, T.I., Kosobruhov, A.A., and Polyakov, V.Yu., Structural organization of chloroplast of tomato plants Solanum lycopersicum transformed by Fe-containing superoxide dismutase, Biochem. (Moscow) Suppl. Ser. A: Membr. Cell Biol., 2011, vol. 5, no. 2, pp. 177–184.

    Article  Google Scholar 

  12. Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant, 1962, vol. 15, pp. 473–497.

    Article  CAS  Google Scholar 

  13. Weekley, B., Electron Microscopy for Beginners, Moscow: Mir, 1975.

    Google Scholar 

  14. Reynolds, E.S., The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol., 1963, vol. 17, pp. 208–212.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Baranova, Ye.N., Akanov, E.N., Gulevich, A.A., Kurenina, L.V., Danilova, S.A., and Khaliluev, M.R., Dark respiration rate of transgenic tomato plants expressing FeSOD1 gene under chloride and sulfate salinity, Russ. Agric. Sci., 2014, vol. 40, no. 1, pp. 14–17.

    Article  Google Scholar 

  16. Altin, O.K., Scheurwater, I., and Pons, T.L., Respiration as a percentage of daily photosynthesis in whole plants in homeostatic at moderate, but not high growth temperatures, New Phytol., 2007, vol. 174, pp. 1–14.

    Article  Google Scholar 

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Authors and Affiliations

  1. All-Russia Research Institute of Agricultural Biotechnology, Moscow, 127550, Russia

    E. N. Baranova, E. K. Nodel’man, L. V. Kurenina, A. A. Gulevich, G. B. Baranova, L. R. Bogoutdinova & M. R. Khaliluev

  2. Williams All-Russia Fodder Research Institute, Lobnya, Moscow oblast, 141005, Russia

    A. A. Gulevich

  3. Pryanishnikov All-Russia Research Institute of Agricultural Chemistry, Moscow, 127550, Russia

    E. N. Akanov

  4. Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, 127550, Moscow, Russia

    L. R. Bogoutdinova & M. R. Khaliluev

Authors
  1. E. N. Baranova
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  2. E. K. Nodel’man
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  3. L. V. Kurenina
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  4. A. A. Gulevich
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  5. G. B. Baranova
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  6. L. R. Bogoutdinova
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  7. E. N. Akanov
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  8. M. R. Khaliluev
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Corresponding author

Correspondence to E. N. Baranova.

Additional information

Original Russian Text © E.N. Baranova, E.K. Nodel’man, L.V. Kurenina, A.A. Gulevich, G.B. Baranova, L.R. Bogoutdinova, E.N. Akanov, M.R. Khaliluev, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 6, pp. @–@.

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Baranova, E.N., Nodel’man, E.K., Kurenina, L.V. et al. Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. under salt stress. Russ. Agricult. Sci. 40, 426–431 (2014). https://doi.org/10.3103/S1068367414060056

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  • DOI: https://doi.org/10.3103/S1068367414060056

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