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Multilocus Sequence Analysis of Isolates from Patients with the Erythemic Form of Ixodid Tick-Borne Borrelioses

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Abstract

Ixodid tick-borne borrelioses (ITBB) is widespread in Russia and ranks first among all natural focal infections in terms of morbidity. More than 50% of them are erythemic. The taxonomic status is clarified of one of the causative agents of the erythemic form of ITBB in Eastern Europe. The method of multilocus sequence analysis (MLSA) was used to study 23 primary isolates of Borrelia obtained by inoculating skin biopsies into BSK medium, as well as blood plasma from patients with the erythemic form of ITBB who had been infected in the Perm region of Russia. According to the MLSA results, the nucleotide sequences of the studied Borrelia isolates differed by at least 2.2–2.7% from the sequences of B. garinii 20047T and turned out to be similar to B. bavariensis PBi by at least 98.3%. The sequences of these isolates were almost identical (98.5–100%) to the strains NT29, SZ, Prm7564-11, Prm7569-11, and Hiratsuka of the Eurasian genomic subgroup of B. bavariensis, which made it possible to assign them to the same subgroup. Among the studied Borrelia isolates, two genovariants were identified that differ from each other by more than 1% in nucleotide sequences, with 99.7–100% similarity between the sequences of each genovariant. Along with B. garinii and B. afzelii, B. bavariensis is widespread in Eurasia and is of great importance as the causative agent of ITBB. The Eurasian and European genomic subgroups of this pathogen circulate in Russia. The Eurasian genomic subgroup of B. bavariensis can cause the erythemic form of ITBB.

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Authors and Affiliations

  1. National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, 123098, Moscow, Russia

    K. A. Golidonova, E. I. Korenberg, N. B. Gorelova & A. L. Ginzburg

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  1. K. A. Golidonova
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  2. E. I. Korenberg
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  3. N. B. Gorelova
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  4. A. L. Ginzburg
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Correspondence to K. A. Golidonova, E. I. Korenberg, N. B. Gorelova or A. L. Ginzburg.

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In carrying out this work, all ethical standards were observed. This paper does not contain any studies involving human beings or animals.

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Translated by A. Ostyak

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Golidonova, K.A., Korenberg, E.I., Gorelova, N.B. et al. Multilocus Sequence Analysis of Isolates from Patients with the Erythemic Form of Ixodid Tick-Borne Borrelioses. Mol. Genet. Microbiol. Virol. 36, 170–175 (2021). https://doi.org/10.3103/S089141682104008X

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