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Multilocus Sequence Analysis of “Atypical” Borrelia burgdorferi sensu lato Isolated in Russia

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Abstract

Seventeen isolates of Borrelia burgdorferi sensu lato with an unclear genetic characteristic isolated in Russia from various species of Ixodes ticks (I. persulcatus, I. ricinus, and I. pavlovskyi) have been studied by multilocus sequencing analysis. According to the results of typing, these isolates were identified as B. bavariensis (n = 7), B. garinii (n = 6), B. finlandensis (n = 3), and B. spielmanii (n = 1), that is, species that can cause ixodid tick-borne borreliosis diseases in human beings. The allelic polymorphism of five genes (rrs, fla, groEL, recA, and ospA) and spacer rrfA–rrlB of B. bavariensis and B. garinii isolates was analyzed in comparison with their diversity in the International Nucleotide Sequence Database Collaboration.

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References

  1. Pritt, B.S., Respicio-Kingry, L.B., Sloan, L.M., Schriefer, M.E., Replogle, A.J., Bjork, J., et al., Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper Midwestern United States, Int. J. Syst. Evol. Microbiol., 2016, vol. 66, no. 11, pp. 4878–4880.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Rudenko, N., Golovchenko, M., Vancova, M., Clark, K., Grubhoffer, L., and Oliver, J.H., Isolation of live Borrelia burgdorferi sensu lato spirochaetes from patients with undefined disorders and symptoms not typical for Lyme borreliosis, Clin. Microbiol. Infect., 2016, vol. 22, no. 3, pp. 267.e9–267.e15. doi 10.1016/j.cmi.2015.11.009

    Article  CAS  Google Scholar 

  3. Mongodin, E.F., Casjens, S.R., Bruno, J.F., Xu, Y., Drabek, E.F., Riley, D.R., et al., Inter-and intra-specific pan-genomes of Borrelia burgdorferi sensu lato: genome stability and adaptive radiation, BMC Genomics, 2013, vol. 14, p.693.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  4. Margos, G., Vollmer, S.A., Ogden, N.H., and Fish, D., Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato, Infect., Genet. Evol., 2011, vol. 11, pp. 1545–1563.

    Article  Google Scholar 

  5. Ivanova, L.B., Tomova, A., González-Acuña, D., Murúa, R., Moreno, C.X., and Hernández, C., Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the Southern Hemisphere, Environ. Microbiol., 2014, vol. 16, no. 4, pp. 1069–1080.

    Article  PubMed  CAS  Google Scholar 

  6. Margos, G., Chu Chen-Yi, Takano Ai, Jiang Bao-Gui, Liu We, Kurtenbach, K., et al., Borrelia yangtzensis sp. nov., a rodent-associated species in Asia, is related to Borrelia valaisiana, Int. J. Syst. Evol. Microbiol., 2015, vol. 65, pp. 3836–3840.

    Article  PubMed  CAS  Google Scholar 

  7. Casjens, S.R., Fraser-Liggett, C.M., Mongodin, E.F., Qiu, W.G., Dunn, J.J., Luft, B.J., and Schutzer, S.E., Whole genome sequence of an unusual Borrelia burgdorferi sensu lato isolate, J. Bacteriol., 2011, vol. 193, no. 6, pp. 1489–1490.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Postic, D., Garnier, M., and Baranton, G., Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates-description of Borrelia californiensis sp. nov., and genomospecies 1 and 2, Int. J. Med. Microbiol., 2007, vol. 297, pp. 263–271.

    Article  PubMed  CAS  Google Scholar 

  9. Vysotskii, V.V. and Kotlyarova, G.A., Poly(hetero) morphic forms of pathogenic bacteria in infectious pathology, Zh. Mikrobiol., Epidemiol. Immunobiol., 1999, no. 2, pp. 100–104.

    Google Scholar 

  10. Korenberg, E.I., Nefedova, V.V., Gorelova, N.B., Kovalevskii, Yu.V., Fadeeva, I.A., and Golubova, D.A., Etiological structure of southern taiga combinations of natural foci of ixodic tick-borne borrelioses, Vestn. Ross. Akad. Med. Nauk, 2011, no. 10, pp. 10–15.

    Google Scholar 

  11. Korenberg, E.I. and Nefedova, V.V., Borrelia—pathogens of tick-borne borrelioses and epidemic relapsing fever, in Rukovodstvo po meditsinskoi mikrobiologii. Chastnaya meditsinskaya mikrobiologiya i etiologicheskaya diagnostika infektsii (Guideline on Medical Microbiology. Special Medical Microbiology and Etiological Diagnostics of Infections), Labinskaya, A.S., Kostyukova, N.N., and Ivanova, S.M., Eds., Moscow: BINOM, 2010, pp. 844–866.

    Google Scholar 

  12. Korenberg, E.I., Pomelova, V.G., and Osin, N.S., Prirodnoochagovye infektsii, peredayushchiesya iksodovymi kleshchami (Natural Focal Infections Transmitted by Ixodic Ticks), Moscow: Kommentarii, 2013.

    Google Scholar 

  13. Margos, G., Vollmer, S.A., Cornet, M., Garnier, M., Fingerle, V., Wilske, B., et al., A new Borrelia species defined by Multilocus sequence analysis of housekeeping genes, Appl. Environ. Microbiol., 2009, vol. 75, no. 16, pp. 5410–5416.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Gorelova, N.B., Korenberg, E.I., Vorob’eva, N.N., Frizen, V.I., Nefedova, V.V., and Teterin, V.Yu., Isolating pathogen of ixodic tick borreliosis from the patients’ blood, Zh. Mikrobiol., Epidemiol. Immunobiol., 2009, no. 1, pp. 63–66.

    Google Scholar 

  15. Nefedova, V.V., Korenberg, E.I., and Gorelova, N.B., Genetic variants of Borrelia garinii, a widespread Eurasian pathogen of ixodic tick borreliosis, Mol. Genet., Microbiol. Virol., 2010, vol. 25, no. 3, pp. 95–100.

    Article  Google Scholar 

  16. Nefedova, V.V., Korenberg, E.I., Fadeeva, I.A., and Gorelova, N.B., Genetic characteristics of pathogenic for humans Borrelia isolated from Ixodes trianguliceps bir. and Ixodes pavlovskyi pom ticks, Med. Parazitol. Parazit. Bolezni, 2005, no. 2, pp. 9–12.

    Google Scholar 

  17. Nefedova, V.V., Korenberg, E.I., and Gorelova, N.B., Genotyping Borrelia burgdorferi sensu lato isolates from Ixodes Ricinus ticks in Russia and Ukraine, Mol. Genet., Microbiol. Virol., 2010, vol. 25, no. 1, pp. 25–30.

    Article  Google Scholar 

  18. Korenberg, E.I., Nefedova, V.V., Romanenko, V.N., and Gorelova, N.B., The tick Ixodes pavlovskyi as a host of spirochetes pathogenic for humans and its possible role in the epizootiology and epidemiology of Borrelioses, Vector Borne Zoonotic Dis., 2010, vol. 10, no. 5, pp. 453–458.

    Article  PubMed  Google Scholar 

  19. Mukhacheva, T.A. and Kovalev, S.Y., Multilocus sequence analysis of Borrelia burgdorferi s.1. in Russia, Ticks Tick Borne Dis., 2013, vol. 4, no. 4, pp. 275–279.

    Article  PubMed  Google Scholar 

  20. Richter, D., Schlee, D.B., Allgöwer, R., and Matuschka, F.-R., Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in Central Europe, Appl. Environ. Microbiol., 2004, vol. 70, no. 11, pp. 6414–6419.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  21. Wang, G., van Dam, A.P., and Dankert, J., Phenotypic and genetic characterization of a novel Borrelia burgdorferi sensu lato isolate from a patient with Lyme borreliosis, J. Clin. Microbiol., 1999, vol. 37, no. 9, pp. 3025–3028.

    PubMed  PubMed Central  CAS  Google Scholar 

  22. Hao, Q., Hou, X., Geng, Z., and Wan, K., Distribution of Borrelia burgdorferi sensu lato in China, J. Clin. Microbiol., 2011, vol. 49, no. 2, pp. 647–650.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Gómez-Díaz, E., Boulinier, T., Sertour, N., Cornet, M., Ferquel, E., and McCoy, K.D., Genetic structure of marine Borrelia garinii and population admixture with the terrestrial cycle of Lyme borreliosis, Environ. Microbiol., 2011, vol. 13, no. 9, pp. 2453–2467.

    Article  PubMed  Google Scholar 

  24. Margos, G., Gatewood, A.G., Aanensen, D.M., Hanincová, K., Terekhova, D., Vollmer, S.A., et al., MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi, Proc. Natl. Acad. Sci. U. S. A., 2008, vol. 105, no. 25, pp. 8730–8735.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Marti Ras, N., Postic, D., Foretz, M., and Baranton, G., Borrelia burgdorferi sensu stricto, a bacterial species “made in the U.S.A.”?, Int. J. Syst. Bacteriol., 1997, vol. 47, no. 4, pp. 1112–1117.

    Article  PubMed  CAS  Google Scholar 

  26. Castillo-Ramírez, S., Fingerle, V., Jungnick, S., Straubinger, R.K., Krebs, S., Blum, H., et al., Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto, Sci. Rep., 2016, vol. 6, p. 22794.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Margos, G., Wilske, B., Sing, A., Hizo-Teufel, C., Cao, W.C., Chu, C., et al., Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia, Int. J. Syst. Evol. Microbiol., 2013, vol. 63, pp. 4284–4288.

    Article  PubMed  Google Scholar 

  28. Huegli, D., Hu, C.M., Humair, P.-F., Wilske, B., and Gern, L., Apodemus species mice are reservoir hosts of Borrelia garinii OspA serotype 4 in Switzerland, J. Clin. Microbiol., 2002, vol. 40, no. 12, pp. 4735–4737.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  29. Takano, A., Nakao, M., Masuzawa, T., Takada, N., Yano, Y., Ishiguro, F., Fujita, H., et al., Multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic Borrelia garinii in Japan, J. Clin. Microbiol., 2011, vol. 49, no. 5, pp. 2035–2039.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Scholz, H.C., Margos, G., Derschum, H., Speck, S., Tserennorov, D., Erdenebat, N., et al., High prevalence of genetically diverse Borrelia bavariensis-like strains in Ixodes persulcatus from Selenge Aimag, Mongolia, Ticks Tick Borne Dis., 2013, vol. 4, nos. 1–2, pp. 89–92.

    Article  Google Scholar 

  31. Filippova, N.A., Taezhnyy kleshch Ixodes persulcatus Schulze (Acrana, Ixodidae): morfologiya, sistematika, ekologiya, meditsinskoe znachenie (Taiga Tick Ixodes persulcatus Schulze (Acrana, Ixodidae): Morphology, Systematics, Ecology, Medical Significance), Leningrad: Nauka, 1985.

    Google Scholar 

  32. Tveten, A.-K., Exploring diversity among Norwegian Borrelia strains originating from Ixodes ricinus ticks, J. Microbiol., 2014, vol. 2014, Art. ID 397143. http://dx.doi.org/. doi 10.1155/2014/397143

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Authors and Affiliations

  1. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russia

    V. V. Nefedova, E. I. Korenberg & N. B. Gorelova

Authors
  1. V. V. Nefedova
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  2. E. I. Korenberg
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  3. N. B. Gorelova
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Correspondence to V. V. Nefedova.

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Original Russian Text © V.V. Nefedova, E.I. Korenberg, N.B. Gorelova, 2017, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2017, No. 4, pp. 145–150.

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Nefedova, V.V., Korenberg, E.I. & Gorelova, N.B. Multilocus Sequence Analysis of “Atypical” Borrelia burgdorferi sensu lato Isolated in Russia. Mol. Genet. Microbiol. Virol. 32, 196–203 (2017). https://doi.org/10.3103/S0891416817040073

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