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Evaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes

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Abstract

In this review, we aim to present an overview of how small-molecular fluorescent substrate probes for studying enzymatic functions are developed and how they are used in biological applications, under the following four headings: (1) History of Visual Detection of Enzymatic Activities, (2) Strategies to Design Fluorescent Substrate Probes to Measure Enzymatic Activities, (3) Development of Fluorescent Substrate Probes Suitable for Biological Studies, and (4) Biological Applications of Fluorescent Substrate Probes for Studying Enzymes.

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Acknowledgments

We would like to thank Prof. Tetsuo Nagano, Assoc. Prof. Kenjiro Hanaoka, and Hiroki Ito for constructive comments for the paper. We also would like to thank W. R. S. Steele for careful proofreading of the paper.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo,Tokyo, 113-0033, Japan

    Toru Komatsu & Yasuteru Urano

  2. Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo,Tokyo, 113-0033, Korea

    Yasuteru Urano

  3. JST PRESTO, 4-1-8 Honcho, Kawaguchi,Saitama, 332-0012, Japan

    Toru Komatsu

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  1. Toru Komatsu
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  2. Yasuteru Urano
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Correspondence to Yasuteru Urano.

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Toru Komatsureceived his Ph.D. in 2009 from the University of Tokyo. He worked at Johns Hopkins University (2009 – 2010) and The Scripps Research Institute (2010) as a postdoc. Currently, he works at the University of Tokyo as an Assistant Professor (2010 – present). He also works as a scientist of Japan Science and Technology Agency (JST), PRESTO. His current research interest is to develop small molecular tools to study enzymatic functions that have specific roles in physiological and pathological states.

Yasuteru Uranoworked as an Assistant Professor (1997 – 2004) and as an Associate Professor (2005 – 2010) of Sciences at the University of Tokyo. Then he became a professor of the Graduate School of Medicine (2010 – present) and the Graduate School of Pharmaceutical Sciences (2014 – present) at the University of Tokyo. He also worked as a scientist of Japan Science and Technology Agency (JST), PRESTO (2004 – 2007). His current research interest is development of novel small molecular-based photo-functional tools for visualizing and controlling important biological activities and phenomena in living cells and animals.

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Komatsu, T., Urano, Y. Evaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes. ANAL. SCI. 31, 257–265 (2015). https://doi.org/10.2116/analsci.31.257

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  • DOI: https://doi.org/10.2116/analsci.31.257

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