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Hyperbranched Polyglycerol Functionalized Silica Stationary Phase for Hydrophilic Interaction Liquid Chromatography

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Abstract

Surface-initiated anionic-ring-opening multibranching polymerization was employed to prepare a hyperbranched polyglycerol (HPG) functionalized silica stationary phase for hydrophilic interaction liquid chromatography (HILIC). The obtained stationary phase was characterized by Fourier-transform infrared spectrometry (FT-IR) and thermogravimetric analysis (TGA). The chromatographic properties of the prepared stationary phase were systematically investigated. The abundance and multitude distribution of hydroxyl groups in HPG endowed the stationary phase with improved hydrophilicity and enhanced separation performance compared with the stationary phase functionalized with monolayer of hydroxyl groups. The stationary phase showed excellent retention of various polar compounds, such as nucleosides, necleobases, phenols and sulfanilamides, indicating great potential in the separation of complex biosamples.

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Acknowledgments

This work has been supported by the National Natural Science Foundation of China (grant No. 21405134, 21575123 and 21675139), the Jiangsu Science and Technology Project (BK20170471), the Talent Introduction Project of Yancheng Institute of Technology (XJ201509) and the Practical Innovation Program for Postgraduates of Specialty Degree in Universities of Jiangsu (SJLX16_0676). This work also sponsored by Jiangsu Qinglan Project and Jiangsu Industry University Foresight Joint Research Project (BY2016065-36).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, No. 9 Yinbing Avenue, Tinghu, Yancheng, 224000, China

    Hengye Li, Xuemeng Zhang, Hui Cang, Fenying Kong, Dahe Fan & Wei Wang

  2. Yancheng Entry-Exit Inspection and Quarantine Bureau, No. 85 Kaifang Avenue, Yancheng, 224000, China

    Lin Zhang

Authors
  1. Hengye Li
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  2. Xuemeng Zhang
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  3. Lin Zhang
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  4. Hui Cang
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  5. Fenying Kong
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  6. Dahe Fan
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  7. Wei Wang
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Correspondence to Dahe Fan or Wei Wang.

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Li, H., Zhang, X., Zhang, L. et al. Hyperbranched Polyglycerol Functionalized Silica Stationary Phase for Hydrophilic Interaction Liquid Chromatography. ANAL. SCI. 34, 433–438 (2018). https://doi.org/10.2116/analsci.17P486

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  • DOI: https://doi.org/10.2116/analsci.17P486

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