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A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography

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Abstract

A hyperbranched stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared by grafting polyethylenimine (PEI) onto silica gel (termed as PEI-Sil). Rich primary, secondary, and tertiary amino groups associated with PEI render its good hydrophility. More importantly, the hyperbranched structure of PEI molecule is greatly helpful in improving interaction with polar analytes. For several kinds of model polar compounds, including organic acids, nucleosides, nucleic acid bases, amino acids, cephalosporins, and non-reducing sugars, PEI-Sil demonstrated excellent separation performance in terms of running stability, reproducibility, and separation efficiency (e.g., plate count ~74,000/m). In addition, PEI-Sil also exhibited much better separation selectivity toward inorganic anions when operated in the mode of ion chromatography relative to a commercial amino propyl-bonded column.

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Acknowledgments

The research was sponsored by the National Natural Science Foundation of China (No. 21322502, 21477037). It also thanks for the support from outstanding young talent cultivation fund of ECUST.

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Authors and Affiliations

  1. Pharmacy School, East China University of Science and Technology, 267 BO, 130 Meilong Road, Shanghai, 200237, China

    Yahui Peng, Yanjie Hou, Feifang Zhang, Guobin Shen & Bingcheng Yang

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  1. Yahui Peng
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  2. Yanjie Hou
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  3. Feifang Zhang
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  4. Guobin Shen
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  5. Bingcheng Yang
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Correspondence to Bingcheng Yang.

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Peng, Y., Hou, Y., Zhang, F. et al. A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography. Anal Bioanal Chem 408, 3633–3638 (2016). https://doi.org/10.1007/s00216-016-9446-7

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  • DOI: https://doi.org/10.1007/s00216-016-9446-7

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