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New easy handling and sampling device for bioavailability screening of topical formulations

  • SPIM 2014 Proceedings
  • Published:
European Journal of Dermatology Aims and scope

Abstract

Biopharmaceutical assessment of topical drug formulations is widely carried out by using vertical diffusion cells (Franz type cell). Although Franz diffusion cell model is well designed for percutaneous absorption studies, the extent of drug penetration within the skin requires more adapted device. Recently, we have developed a new patented versatile, easy-to-use, and disposable diffusion cell called VitroPharma. In this study we have assessed the cutaneous bioavailability of caffeine as hydrophilic compound model using Franz diffusion cell and VitroPharma. The percutaneous absorption of caffeine assessed with Franz diffusion cell andVitroPharmawas characterized by using (i) finite dose model and (ii) classical pharmacokinetic analysis. Furthermore, the follow-up of caffeine penetration within the skin was determined by sequential measurements of tissular drug concentration throughout the time of skin exposure with VitroPharma. However, classical experimental design using Franz diffusion cell involved unique determination of tissular concentration at the final point of skin exposure protocol. Finally, device equivalence between Franz diffusion cell and VitroPharma was claimed from percutaneous absorption data analysis. Concomitant assessment of dual penetration and permeation kinetics by using VitroPharma reinforced the understanding of skin drug delivery.

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Authors and Affiliations

  1. EA 4169: Aspect fondamentaux, cliniques et thérapeutiques de la fonction barrière cutanée, Laboratoire de Pharmacie Galénique Industrielle, Plateforme FRIPHARM, Faculté de Pharmacie, SFR Lyon-Est Santé, Université Claude Bernard Lyon 1., 8, avenue Rockefeller, 69373, Lyon cedex 08, France

    Damien Salmon, Elodie Gilbert, Marek Haftek & Fabrice Pirot

  2. Unité de préparation et contrôle des médicaments, Service Pharmaceutique, Plateforme FRIPHARM, Groupement Hospitalier Edouard Herriot, Hospices Civils de Lyon., 5, Place d’Arsonval, 69437, Lyon cedex 03, France

    Damien Salmon, Bruna Gioia, Christine Pivot & Fabrice Pirot

  3. UMR 5305: Laboratoire de Biologie Tissulaire et d’Ingénierie Thérapeutique, CNRS/Université Claude Bernard Lyon 1, Institut de Biologie et Chimie des Protéines, 7, Passage du Vercors, 69367, Lyon Cedex 07, France

    Bernard Verrier

Authors
  1. Damien Salmon
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  2. Elodie Gilbert
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  3. Bruna Gioia
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  4. Marek Haftek
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  5. Christine Pivot
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  6. Bernard Verrier
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  7. Fabrice Pirot
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Corresponding author

Correspondence to Damien Salmon.

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Salmon, D., Gilbert, E., Gioia, B. et al. New easy handling and sampling device for bioavailability screening of topical formulations. Eur J Dermatol 25 (Suppl 1), 23–29 (2015). https://doi.org/10.1684/ejd.2015.2551

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  • DOI: https://doi.org/10.1684/ejd.2015.2551

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