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Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato

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Journal of Zhejiang University-SCIENCE B Aims and scope Submit manuscript

Abstract

Objectives

To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress.

Methods

Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 °C, 500 μmol/(m2·s); Part 2, spayed with distilled water (H2O) under 35 °C, 1000 μmol/(m2·s) (HH); Part 3, spayed with 100 μmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 μmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d.

Results

HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F v/F m), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETRI and Y I) significantly decreased whereas acceptor and donor side limitation of PSI (Y NA and Y ND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy Y NPQ and Y NO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions.

Conclusion

The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.

中文概要

题目

线性电子传递和环式电子传递对缓解番茄亚高温强光胁迫的响应

目的

探讨特定高温和强光逆境下番茄叶片中的环式电子传递(CEF)和线性电子传递(LEF)的光保护机制。

创新点

通过引入电子抑制剂的方法系统分析了CEF 和LEF 对亚高温强光胁迫的响应。

方法

将品种为“辽园多丽”的番茄幼苗(n=160)平 均分成四组(表1):组1,于常温常光照25 °C, 500 μmol/(m2·s)条件下培养并作为对照;组2, 叶片喷施蒸馏水并在亚高温强光35 °C , 1000 μmol/(m2·s)(HH)条件下培养;组3,HH 条件下叶片喷施100 μmol/L 敌草隆(DCMU,CEF 抑制剂);组4,HH 条件下叶片喷施60 μmol/L 甲基紫精(MV,LEF 抑制剂)。在处理5 d 及恢 复10 d期间,分别测定番茄幼苗叶片的光能吸收、 激发能分配、光系统活性、类囊体膜完整性和ATP 酶活性等指标。

结论

CEF 和LEF 通过一定程度上改善叶片光能吸收及 激发能分配,并且维持类囊体膜较高完整性和 ATP 酶活性,从而维持光系统活性并减少光抑制 和光破坏程度。

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Authors and Affiliations

  1. College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China

    Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu & Tian-lai Li

  2. Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, 110866, China

    Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu & Tian-lai Li

  3. Collaborative Innovation Center of Protected Vegetable Surrounds Bohai Gulf Region, Shenyang, 110866, China

    Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu & Tian-lai Li

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  1. Tao Lu
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Project supported by the China Agriculture Research System (No. CARS-25), the National Natural Science Foundation of China (No. 31301813), and the National Key Technologies R & D Program of China (No. 2015103003)

ORCID: Tao LU, http://orcid.org/0000-0003-0098-4356

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Lu, T., Shi, Jw., Sun, Zp. et al. Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato. J. Zhejiang Univ. Sci. B 18, 635–648 (2017). https://doi.org/10.1631/jzus.B1600286

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