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Effects of neutral phytase on growth performance and phosphorus utilization in crucian carp (Carassius auratus)

中性植酸酶对鲫鱼生长及磷利用的影响

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Abstract

A feeding trial was conducted for nine weeks to investigate the effects of partially replacing Ca(H2PO4)2 with neutral phytase on the growth performance, phosphorus utilization, nutrient digestibility, serum biochemical parameters, bone and carcass mineral composition, and digestive-enzyme-specific activity in crucian carp (Carassius auratus). The diets prepared with 0.8%, 0%, and 1.8% Ca(H2PO4)2 (1%=1 g/100 g) supplements were regarded as the P1E0, negative control (NC), and positive control (PC) groups, respectively; the other three experimental diets were prepared with the addition of 200, 300, and 500 U/kg of neutral phytase, respectively, based on the P1E0 group. Three hundred and eighty-four fish ((1.50±0.01) g) were randomly distributed in the six treatments with four replicates each. The fish were initially fed with 2%–3% diets of their body weight per day, with feeding twice daily (08:00 and 16:00), under a 12-h light/12-h dark cycle at the temperature of (27.56±0.89) °C. The results showed that supplemental phytase at different levels in the diet improved the final body weight, average daily gain, feed conversion ratio, phosphorus utilization, and protein efficiency ratio of crucian carp (P<0.05). Phytase supplementation increased the mineral content in serum (P), bone (P, Ca), and carcass (P, Ca, Zn, Na, and Mg) (P<0.05); the trypsin and chymotrypsin activity soared when fed with the phytase-supplemented diets (P<0.05). We may conclude that supplemental dietary neutral phytase improved the growth performance, phosphorus utilization as well as nutrient utilization in crucian carp, and it can be considered an important nutritional replacement for Ca(H2PO4)2.

摘要

目 的

比较研究在无机磷 (磷酸二氢钙) 添加不足时, 添加不同剂量中性植酸酶对鲫鱼生长和磷利用的影响, 为水产养殖更好地应用植酸酶提供依据并减少磷的排放, 保护环境。

创新点

试验采用的中性植酸酶能够耐受饲料加工温度, 并从营养物质消化及磷利用等方面研究中性植酸酶对鲫鱼的影响, 探究低磷日粮在水产养殖的应用。

方 法

384 条鲫鱼 ((1.50±0.01) g) 随机分为 6 组, 分别饲喂对照组 (未添加中性植酸酶) 跟试验组 (添加不同剂量中性植酸酶) 日粮 63 天, 比较 3 组间各项指标差异。

结 论

在以植物蛋白为主的日粮中, 添加中性植酸酶能够促进植酸磷水解, 提高鲫鱼对饲粮中磷、 粗蛋白等营养物质的利用, 促进钙、 磷等矿物质在鲫鱼体中的沉积, 降低粪磷排出量, 促进鲫鱼生长。说明可以在鲫鱼日粮中添加中性植酸酶, 减少无机磷的添加, 降低粪磷排放, 从而减轻环境污染。

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Authors and Affiliations

  1. Key Laboratory of Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China

    Xin-zheng Nie, Sha Chen, Xiao-xu Zhang, Bin-yang Dai & Li-chun Qian

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  1. Xin-zheng Nie
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  2. Sha Chen
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  3. Xiao-xu Zhang
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Correspondence to Li-chun Qian.

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Project supported by the National High-Tech R&D Program (863) of China (No. 2013AA102803)

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Nie, Xz., Chen, S., Zhang, Xx. et al. Effects of neutral phytase on growth performance and phosphorus utilization in crucian carp (Carassius auratus). J. Zhejiang Univ. Sci. B 18, 886–896 (2017). https://doi.org/10.1631/jzus.B1600280

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