Abstract
The dietary calcium requirement of fingerling Labeo rohita (6.0 ± 0.11 cm; 1.94 ± 0.07 g) was examined using seven iso-nitrogenous and iso-caloric purified diets (350 g kg−1 crude protein; 16.72 kJ g−1 gross energy) with incremental levels of calcium (2.14, 3.12, 4.23, 5.11, 6.14, 8.23, and 11.24 g kg−1) that were hand-fed to triplicate groups of fish (20 fish per tank) to apparent satiation for 8 weeks. At the end of the feeding trial, ten fish from each replicate tank (n = 10 × 3) were sampled to obtain serum, scales, and vertebrae. The remaining fish in each tank were sampled to determine proximate chemical body composition. Live weight gain (LWG; 637.6%), protein gain (PG; 2.19 g fish−1), protein retention efficiency (29.89%), and feed conversion ratio (1.69) improved with increased dietary calcium up to 5.11 g kg−1 and then stabilized. Dietary calcium up to 5.11 g kg−1 significantly increased calcium and phosphorus concentrations of whole body, vertebrae, and scales. Fish fed a diet with calcium higher than 5.11 g kg−1 had a significant decrease (P < 0.05) in magnesium and zinc, while potassium was unaffected. Serum alkaline phosphatase activity increased (P < 0.05) with increased dietary calcium up to 5.11 g kg−1, but serum calcium and phosphorus concentrations remained unchanged. Based on broken-line regression analysis of LWG, PG, whole body, vertebrae, and scale calcium content against dietary calcium concentration, dietary calcium in the range of 5.16–5.48 g kg−1 is recommended for fingerling L. rohita.
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We are grateful to the Chairman of our department for providing laboratory facilities.
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Financial assistance was provided by University Grants Commission (Grant No. F. 53-2/2013 (CU)) to Mohammad Musharraf.
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Musharraf, M., Khan, M.A. Dietary calcium requirement of fingerling Indian major carp, Labeo rohita (Hamilton) based on growth performance, tissue mineralization, whole body, and serum biochemical composition. Aquacult Int 28, 1125–1139 (2020). https://doi.org/10.1007/s10499-020-00515-2
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DOI: https://doi.org/10.1007/s10499-020-00515-2