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Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake

松茸多糖的超声波提取优化、结构特性和抗氧化活性研究

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Abstract

An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholoma matsutake fruiting bodies. Single-factor tests and orthogonal experimental design (L 9(33)) were used to obtain the optimal extraction conditions. Results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °C; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power, 100 W. Three novel T. matsutake polysaccharide (TMP) fractions (TMP30, TMP60, and TMP80) were isolated and purified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD) and Fourier transform infrared spectrophotometer (FT-IR) analyses. Furthermore, their in vitro antioxidant activity was investigated in terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. The order of the various fractions based on their antioxidant activity was TMP80>TMP>TMP60>TMP30. These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be promising active macromolecules for biomedical use.

摘要

目 的

对超声提取松茸多糖(TMP)的工艺条件进行优化, 对TMP 进一步分离纯化, 并对纯化多糖(TMP30、TMP60和TMP80)的结构和抗氧化活性进行评估。

创新点

首次对超声辅助制备的TMP 进行了乙醇分级, 对其进行了结构和抗氧化活性评估, 并筛选出了抗氧化活性最强的部位(TMP80)。

方 法

采用单因素试验和正交试验(L 9(33))对超声提取TMP 的工艺条件进行了优化; 采用不同的乙醇终浓度对TMP 分离纯化为3种多糖TMP30、TMP60和TMP80; 采用高效阴离子色谱(HPAEC-PAD)和傅里叶红外(FT-IR)等技术对3种多糖的理化性质进行了结构表征; 利用还原力测定、二苯三硝基苯肼(DPPH)和羟自由基的清除作用对它们的抗氧化活性进行了评估。

结 论

正交试验结果表明, 超声提取TMP 的最优工艺条件为: 超声温度40 °C, 超声时间50 min, 水原料比例25 ml/g, 超声波频率45 kHz 和超声波功率100 W(表1和表2)。在此条件下的TMP 得率为8.06%。采用乙醇分级法对TMP进一步分离纯化, 制备得到3种多糖TMP30、TMP60和TMP80。HPAEC-PAD 和FT-IR 的结果表明, TMP30、TMP60和TMP80均主要由岩藻糖(l-Fuc)、半乳糖(d-Gal)、葡萄糖(d-Glc)、木糖(d-Xyl)和甘露糖(d-Man)组成, 它们具有相同的单糖组成, 但含有不同的摩尔比, 且均有β-构型(图2、图3和表3)。抗氧化活性结果表明, 它们的能力高低顺序为: TMP80>TMP>TMP60>TMP30(图4和表4), TMP80抗氧化活性最高, 为松茸多糖的进一步开发利用提供重要的科学依据。

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Authors and Affiliations

  1. College of Life Science, Liaocheng University, Liaocheng, 252059, China

    Yun Chen, Xiu-ju Du, Yang Zhang & Xuan-dong Wang

  2. Agricultural Science Institute of Liaocheng, Liaocheng, 252000, China

    Xin-hua Liu

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  1. Yun Chen
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Correspondence to Xiu-ju Du.

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Project supported by the Natural Science Foundation of Shandong Province of China (No. ZR2015CL002) and the Doctoral Research Startup Foundation of Liaocheng University (No. 31805), China

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Chen, Y., Du, Xj., Zhang, Y. et al. Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake . J. Zhejiang Univ. Sci. B 18, 674–684 (2017). https://doi.org/10.1631/jzus.B1600239

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