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Abnormal cell-selective surface modification by phenylboronic acid functionalized carbon dots on hierarchical bioceramic coating

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Abstract

A challenge of bone implants is to create a cell-selective surface that promotes the physiological activities of normal cells but inhibits those of abnormal cells. We produced phenylboronic acid (PBA) functionalized carbon dots using PBA to create hierarchical bioceramic coatings using a one-step fabrication method. Hydrothermal treatment at 150, 175, and 200 °C for 24 h was used to form carbon-containing nano-hydroxyapatite upon micro-scaled micro-arc-oxidation coatings. Sample microstructures and surface chemical compositions were analyzed, and in vitro cell tests included assessments of cell proliferation, adhesion observation, and osteogenic differentiation. The proposed hierarchical bioceramic coatings indeed promoted MC3T3-E1 cell proliferation and osteogenic differentiation, but inhibited osteosarcoma MG63 cell growth. This study proposed a potential surface modification method that can prevent the rapid growth of abnormal cells and be used for patients with neoplastic disease.

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Abnormal cell-selective surface modification by phenylboronic acid functionalized carbon dots on hierarchical bioceramic coating.

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References

  1. R. Gao-hong, L. Run-guang, J. Gui-yong, C. Chao-jie, B. Zhi-gang, A solution to the vessel shortage during free vascularized fibular grafting for reconstructing infected bone defects of the femur: Bridging with vein transplantation. Injury 48(2), 486 (2017)

    Article  Google Scholar 

  2. T.G. Scott, G. Blackburn, M. Ashley, I.S. Bayer, A. Ghosh, A.S. Biris, A. Biswas, Advances in bionanomaterials for bone tissue engineering. J. Nanosci. Nanotechnol. 13(1), 1 (2013)

    Article  CAS  Google Scholar 

  3. M. Afifi, M.E. El-Naggar, S. Muhammad, N.A. Alghamdi, S. Wageh, S.R. Salem, D. Alhashmialameer, M.A. Taleb, Nanocomposites based on hydroxyapatite/lithium oxide and graphene oxide nanosheets for medical applications. J. Mater. Sci. 57(24), 11300 (2022)

    Article  CAS  Google Scholar 

  4. G. Blackburn, T.G. Scott, I.S. Bayer, A. Ghosh, A.S. Biris, A. Biswas, Bionanomaterials for bone tumor engineering and tumor destruction. J. Mater. Chem. B 1(11), 1519 (2013)

    Article  CAS  Google Scholar 

  5. T. Sattar, T. Manzoor, F.A. Khalid, M. Akmal, G. Saeed, Improved in vitro bioactivity and electrochemical behavior of hydroxyapatite-coated NiTi shape memory alloy. J. Mater. Sci. 54(9), 7300 (2019)

    Article  CAS  Google Scholar 

  6. A. Biswas, I.S. Bayer, H. Zhao, T. Wang, F. Watanabe, A.S. Biris, Design and synthesis of biomimetic multicomponent all-bone-minerals bionanocomposites. Biomacromol 11(10), 2545 (2010)

    Article  CAS  Google Scholar 

  7. Y. Liu, Z. Dang, Y. Wang, J. Huang, H. Li, Hydroxyapatite/graphene-nanosheet composite coatings deposited by vacuum cold spraying for biomedical applications: inherited nanostructures and enhanced properties. Carbon 67, 250 (2014)

    Article  CAS  Google Scholar 

  8. S.S. Rao, J.M. El Abiad, V. Puvanesarajah, A.S. Levin, L.C. Jones, C.D. Morris, Osteonecrosis in pediatric cancer survivors: Epidemiology, risk factors, and treatment. Surg. Oncol. 28, 214 (2019)

    Article  Google Scholar 

  9. H. Liu, Y. Li, K. Sun, J. Fan, P. Zhang, J. Meng, S. Wang, L. Jiang, Dual-responsive surfaces modified with phenylboronic acid-containing polymer brush to reversibly capture and release cancer cells. J. Am. Chem. Soc. 135(20), 7603 (2013)

    Article  CAS  Google Scholar 

  10. M. Zhang, Q. Wang, Y. Xu, L. Guo, Z. Lai, Z. Li, Graphitic carbon nitride quantum dots as analytical probe for viewing sialic acid on the surface of cells and tissues. Anal. Chim. Acta. 1095, 204 (2020)

    Article  CAS  Google Scholar 

  11. K. Ghosal, A. Ghosh, Carbon dots: the next generation platform for biomedical applications. Mater. Sci. Eng. C 96, 887 (2019)

    Article  CAS  Google Scholar 

  12. B. Geng, D. Yang, D. Pan, L. Wang, F. Zheng, W. Shen, C. Zhang, X. Li, NIR-responsive carbon dots for efficient photothermal cancer therapy at low power densities. Carbon 134, 153 (2018)

    Article  CAS  Google Scholar 

  13. Y. Shu, J. Lu, Q.-X. Mao, R.-S. Song, X.-Y. Wang, X.-W. Chen, J.-H. Wang, Ionic liquid mediated organophilic carbon dots for drug delivery and bioimaging. Carbon 114, 324 (2017)

    Article  CAS  Google Scholar 

  14. Y. Shi, Y. Pan, J. Zhong, J. Yang, J. Zheng, J. Cheng, R. Song, C. Yi, Facile synthesis of gadolinium (III) chelates functionalized carbon quantum dots for fluorescence and magnetic resonance dual-modal bioimaging. Carbon 93, 742 (2015)

    Article  CAS  Google Scholar 

  15. S. Sivaselvam, C. Viswanathan, N. Ponpandian, One-step preparation of N-doped grapheme quantum dots with high quantum yield for bioimaging and highly sensitive electrochemical detection of isoniazid. Biomater. Adv. 135, 212731 (2022)

    Article  CAS  Google Scholar 

  16. L. Wu, Y. Gao, C. Zhao, D. Huang, W. Chen, X. Lin, A. Liu, L. Lin, Synthesis of curcumin-quaternized carbon quantum dots with enhanced broad-spectrum antibacterial activity for promoting infected wound healing. Biomater. Adv. 133, 112608 (2022)

    Article  Google Scholar 

  17. A. Santos-Coquillat, E. Martínez-Campos, M. Mohedano, R. Martínez-Corriá, V. Ramos, R. Arrabal, E. Matykina, In vitro and in vivo evaluation of PEO-modified titanium for bone implant applications. Surf. Coat. Technol. 347, 358 (2018)

    Article  CAS  Google Scholar 

  18. K.-C. Kung, K. Yuan, T.-M. Lee, T.-S. Lui, Effect of heat treatment on microstructures and mechanical behavior of porous Sr–Ca–P coatings on titanium. J. Alloys Compd. 515, 68 (2012)

    Article  CAS  Google Scholar 

  19. X. Lu, Y. Li, W. Feng, S. Guan, P. Guo, Self-healing hydroxypropyl guar gum/poly (acrylamide-co-3-acrylamidophenyl boronic acid) composite hydrogels with yield phenomenon based on dynamic PBA ester bonds and H-bond. Colloids Surf. A 561, 325 (2019)

    Article  CAS  Google Scholar 

  20. X. Zhao, L. Dong, Y. Ming, M. Wang, Z. Lu, Y. Xu, H. Li, A magnetofluorescent boron-doped carbon dots as a metal-free bimodal probe. Talanta 200, 9 (2019)

    Article  CAS  Google Scholar 

  21. Y. Zhang, H. Jing, T. Wen, Y. Wang, Y. Zhao, X. Wang, X. Qian, W. Ying, Phenylboronic acid functionalized C3N4 facultative hydrophilic materials for enhanced enrichment of glycopeptides. Talanta 191, 509 (2019)

    Article  CAS  Google Scholar 

  22. S. Wang, Q. Jia, Y. Liu, X. Jing, An investigation on the effect of phenylboronic acid on the processibilities and thermal properties of bis-benzoxazine resins. React. Funct. Polym. 93, 111 (2015)

    Article  CAS  Google Scholar 

  23. S. Wang, X. Jing, Y. Wang, J. Si, High char yield of aryl boron-containing phenolic resins: the effect of phenylboronic acid on the thermal stability and carbonization of phenolic resins. Polym. Degrad. Stab. 99, 1 (2014)

    Article  CAS  Google Scholar 

  24. S. Pang, Y. He, P. He, X. Luo, Z. Guo, H. Li, Fabrication of two distinct hydroxyapatite coatings and their effects on MC3T3-E1 cell behavior. Colloids Surf. B 171, 40 (2018)

    Article  CAS  Google Scholar 

  25. Q. Lin, D. Huang, J. Du, Y. Wei, Y. Hu, X. Lian, X. Xie, W. Chen, Y.S. Zhang, Nano-hydroxyapatite crystal formation based on calcified TiO2 nanotube arrays. Appl. Surf. Sci. 478, 237 (2019)

    Article  CAS  Google Scholar 

  26. A. Saranti, A. Tiron-Stathopoulos, L. Papaioannou, C. Gioti, A. Ioannou, M.A. Karakassides, K. Avgoustakis, I. Koutselas, K. Dimos, 3D-printed bioactive scaffolds for bone regeneration bearing carbon dots for bioimaging purposes. Smart Mater. Med. 3, 12 (2022)

    Article  Google Scholar 

  27. H.A. Badr, D.M.M. AlSadek, M.P. Mathew, C.-Z. Li, L.B. Djansugurova, K.J. Yarema, H. Ahmed, Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation. Biomaterials 70, 23 (2015)

    Article  CAS  Google Scholar 

  28. L. Liang, Y. Shen, J. Zhang, S. Xu, W. Xu, C. Liang, B. Han, Identification of breast cancer through spectroscopic analysis of cell-membrane sialic acid expression. Anal. Chim. Acta 1033, 148 (2018)

    Article  CAS  Google Scholar 

  29. Y.-N. Wang, W.-S. Zhang, X.-P. Liu, Y.-Y. Wei, Z.-R. Xu, A nanohybrid of Prussian blue supported by boracic acid-modified g-C3N4 for Raman recognition of cell surface sialic acid and photothermal/photodynamic therapy. Colloids Surf. B 215, 112490 (2022)

    Article  CAS  Google Scholar 

  30. S. Xu, S. Che, P. Ma, F. Zhang, L. Xu, X. Liu, X. Wang, D. Song, Y. Sun, One-step fabrication of boronic-acid-functionalized carbon dots for the detection of sialic acid. Talanta 197, 548 (2019)

    Article  CAS  Google Scholar 

  31. P. Sadhukhan, M. Kundu, S. Chatterjee, N. Ghosh, P. Manna, J. Das, P.C. Sil, Targeted delivery of quercetin via pH-responsive zinc oxide nanoparticles for breast cancer therapy. Mater. Sci. Eng. C 100, 129 (2019)

    Article  CAS  Google Scholar 

  32. L. Cheng, X. Zhang, Z. Zhang, H. Chen, S. Zhang, J. Kong, Multifunctional phenylboronic acid-tagged fluorescent silica nanoparticles via thiol-ene click reaction for imaging sialic acid expressed on living cells. Talanta 115, 823 (2013)

    Article  CAS  Google Scholar 

  33. H. Zhou, M. Yang, S. Hou, L. Deng, Mesoporous hydroxyapatite nanoparticles hydrothermally synthesized in aqueous solution with hexametaphosphate and tea polyphenols. Mater. Sci. Eng. C 71, 439 (2017)

    Article  CAS  Google Scholar 

  34. I.H. Chen, T.M. Lee, C.L. Huang, Biopolymers hybrid particles used in dentistry. Gels (Basel, Switzerland) 7(1), 31 (2021)

    Google Scholar 

  35. K.-C. Kung, T.-M. Lee, T.-S. Lui, Bioactivity and corrosion properties of novel coatings containing strontium by micro-arc oxidation. J. Alloys Compd. 508(2), 384 (2010)

    Article  CAS  Google Scholar 

  36. P. Shen, Y. Xia, Synthesis-modification integration: one-step fabrication of boronic acid functionalized carbon dots for fluorescent blood sugar sensing. Anal. Chem. 86(11), 5323 (2014)

    Article  CAS  Google Scholar 

  37. B.L. Thiel, M. Toth, Secondary electron contrast in low-vacuum∕environmental scanning electron microscopy of dielectrics. J. Appl. Phys. 97(5), 051101 (2005)

    Article  Google Scholar 

  38. J.L. Baker, L.H. Jimison, S. Mannsfeld, S. Volkman, S. Yin, V. Subramanian, A. Salleo, A.P. Alivisatos, M.F. Toney, Quantification of thin film crystallographic orientation using x-ray diffraction with an area detector. Langmuir 26(11), 9146 (2010)

    Article  CAS  Google Scholar 

  39. Y.-T. Liu, K.-C. Kung, C.-Y. Yang, T.-M. Lee, T.-S. Lui, Engineering three-dimensional structures using bio-inspired dopamine and strontium on titanium for biomedical application. J. Mater. Chem. B 2(45), 7927 (2014)

    Article  CAS  Google Scholar 

  40. S.-P. Yang, H.-S. Wen, T.-M. Lee, T.-S. Lui, Cell response on the biomimetic scaffold of silicon nano- and micro-topography. J. Mater. Chem. B 4(10), 1891 (2016)

    Article  CAS  Google Scholar 

  41. D.-Y. Qian, G.-B. Yan, B. Bai, Y. Chen, S.-J. Zhang, Y.-C. Yao, H. Xia, Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells. Biomed. Pharmacother. 90, 492 (2017)

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge the financial support provided to this study by the Ministry of Science and Technology (MOST) in Taiwan under Grant Nos. MOST‐ 109-2314-B-006-012-MY3

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Authors and Affiliations

  1. Institute of Oral Medicine, National Cheng Kung University, Tainan, 701, Taiwan

    Tzer-Min Lee & Nai-Wei Kuo

  2. School of Dentistry, National Cheng Kung University, Tainan, 701, Taiwan

    Tzer-Min Lee

  3. Taiwan Instrument Research Institute, National Applied Research Laboratories, Hsinchu, 300, Taiwan

    Tzer-Min Lee

  4. Center for Fundamental Science, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan

    Chih-Ling Huang

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  1. Tzer-Min Lee
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Correspondence to Chih-Ling Huang.

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Lee, TM., Kuo, NW. & Huang, CL. Abnormal cell-selective surface modification by phenylboronic acid functionalized carbon dots on hierarchical bioceramic coating. Journal of Materials Research 38, 654–663 (2023). https://doi.org/10.1557/s43578-022-00847-0

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